Development of the T-ALLiPSC-based Therapeutic Cancer Vaccines for T-cell Acute Lymphoblastic Leukemia

Abstract

The T-cell acute lymphoblastic leukemia (T-ALL) is a kind of hematological malignancy in children. Despite the significant improvement in the cure rate of T-ALL upon treatment with chemotherapy regimens, steroids, and allotransplantation there are relapses. This study focuses on the tumor-specific therapeutic vaccines derived from the induced pluripotent stem cells (iPSC) to address the issue of T-ALL recurrence. Patient-derived tumor cells and healthy donor cells were reprogrammed into the iPSCs and the RNA-seq data of the T-ALL-iPSCs and H-iPSCs were analyzed. In vitro, the whole cell lysate antigens of iPSCs were prepared to induce the dendritic cells (DC) maturation, which in turn stimulated the tumor-specific T cells to kill the T-ALL tumor cells (Jurkat, CCRF-CEM, MOLT-4). The cytotoxic T lymphocyte (CTL) stimulated by the DC-loaded T-ALL-iPSC-derived antigens showed specific cytotoxicity against the T-ALL cells in vitro. In conclusion, the T-ALL-iPSC-based therapeutic cancer vaccine can elicit a specific anti-tumor effect on T-ALL.

Directed flow and assembly of magnetic polymer nanocomposites switchable between steady and non-steady status driven by magnetic field

“…To date, stimuli-responsive polymeric materials including shape-memory polymers [16][17][18][19], smart hydrogels [20,21], and liquid crystal elastomers [22,23] have been widely developed and have been used to create switchable topography on their surfaces [24][25][26][27]. Shape-memory effect (SME) has been used to create tunable wrinkles and patterned topography on surfaces of polymers with suitable architectures [27][28][29][30]. Although shape change in surface topography upon heating can find great applications, the change cannot occur again unless another shape-memory creation procedure is performed.…”