Development of the Single-Cell MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) Mass-Spectroscopic Assay

Shimizu, Masakazu; Ojima, Noriyuki; Ohnishi, Hitoshi; Shingaki, Tomoteru; Hirakawa, Yasuyuki; Masujima, Tsutomu

doi:10.2116/analsci.19.49

Cited by 24 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These researchers presented an early study in which histamine was detected in single bone-marrow-derived mast cells [72]. The single cells were initially sucked into a microcapillary and released onto a hydrophobic MALDI plate where cocrystallization with a MALDI matrix took place.…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Analytical techniques for single-cell metabolomics: state of the art and trends

2010

View full text Add to dashboard Cite

Single-cell metabolomics is an emerging field that addresses fundamental biological questions and allows one to observe metabolic phenomena in heterogeneous populations of single cells. In this review, we assess the suitability of different detection techniques and present considerations on sample preparation for single-cell metabolomics. Although targeted analysis of single cells can readily be conducted using fluorescent probes and optical instruments (microscopes, fluorescence detectors), a comprehensive metabolomic approach requires a powerful label-free method, such as mass spectrometry (MS). Mass-spectrometric techniques applied to study small molecules in single cells include electrospray MS, matrix-assisted laser desorption/ionization MS, and secondary ion MS. Sample preparation is an important aspect to be taken into account during further development of methods for single-cell metabolomics.

show abstract

Section: Electrochemical Detectionmentioning

confidence: 99%

Analytical techniques for single-cell metabolomics: state of the art and trends

2010

View full text Add to dashboard Cite

show abstract

“…Our group also reported that small molecules such as histamine can be detected in a mast cell using a similar method. 19,20) Zenobi's group performed bacterial single-cell MALDI-MS showing cellular heterogeneity. 21) …”

Section: Current Topicsmentioning

confidence: 99%

Molecular and Functional Analysis of Cellular Phenomena Using Single-Cell Mass Spectrometry

Tsuyama

Mizuno

Masujima

2012

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorption/ionization imaging mass spectrometry and secondary-ion mass spectrometry use in situ ionization of specimens on sample plates to visualize molecular distributions as images from mass spectra. Several single-cell mass spectrometry technologies that initially recover a single cell followed by ionization have been developed. Among them, only nanospray-mediated sampling and ionization named Live Single-cell Mass Spectrometry can be used for real-time analysis. This paper explains that method in detail.

show abstract

“…For many types of cells and applications, no special treatment (such as extraction) needs to be applied to the cell. In the case of MALDI, the cell is normally mixed with a MALDI matrix solution on the target plate (e.g., van Veelen et al, 1994;van Strien et al, 1996;Dahlgren, Page, & Sweedler, 1999;Li, Garden, & Sweedler, 2000a;Shimizu et al, 2003;Rubakhin et al, 2006): this approach requires minimum sample preparation and seems to be applicable to analysis of multiple and single biological cells. The method is seen to require less sample manipulation and it can even provide information on spatial localization of analytes in the studied tissues (DeKeyser & Li, 2007).…”

Section: On-plate Sample Extractionmentioning

confidence: 99%

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Urban

Amantonico

Zenobi

2011

Mass Spectrometry Reviews

View full text Add to dashboard Cite

We review the literature that describes how (matrix-assisted) laser desorption/ionization (MA)LDI target plates can be used not only as sample supports, but beyond that: as functional parts of analytical protocols that incorporate detection by MALDI-MS or matrix-free LDI-MS. Numerous steps of analytical procedures can be performed directly on the (MA)LDI target plates prior to the ionization of analytes in the ion source of a mass spectrometer. These include homogenization, preconcentration, amplification, purification, extraction, digestion, derivatization, synthesis, separation, detection with complementary techniques, data storage, or other steps. Therefore, we consider it helpful to define the "lab-on-a-plate" as a format for carrying out extensive sample treatment as well as bioassays directly on (MA)LDI target plates. This review introduces the lab-on-plate approach and illustrates it with the aid of relevant examples from the scientific and patent literature.

show abstract

Development of the Single-Cell MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) Mass-Spectroscopic Assay

Cited by 24 publications

References 20 publications

Analytical techniques for single-cell metabolomics: state of the art and trends

Analytical techniques for single-cell metabolomics: state of the art and trends

Molecular and Functional Analysis of Cellular Phenomena Using Single-Cell Mass Spectrometry

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Contact Info

Product

Resources

About