Development of the Sf-RNase Gene-Specific PCR Primer Set for Japanese Apricot (Prunus mume Sieb. et Zucc.)

龍太郎, 田尾; 梓, 難波; 久代, 山根; 吉朗, 冬廣; 毅, 渡邊; 剛, 羽生; 明, 杉浦

doi:10.2503/hrj.2.237

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…Tao et al (2002b) cloned the full-length cDNAs for S-RNase alleles of 'Nanko' and showed they had sequence similarity to other Prunus S-RNases. Tao et al (2000Tao et al ( , 2002aTao et al ( , 2002bTao et al ( , 2003 and Habu et al (2008) (Table 9). Yamane et al (2003d) cloned SFB 1 and SFB 7 from the SI cultivar 'Nanko' using rapid amplification of the cDNA ends (RACE) technique with SFB gene-specific primers (Yamane et al, 2003b).…”

Section: Subgenus Prunofora (Japanese Apricot Apricot Andmentioning

confidence: 99%

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Yamane

Tao

2009

J. Japan. Soc. Hort. Sci.

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Most rosaceous fruit tree species exhibit gametophytic self-incompatibility (GSI). During the last decade, much progress has been made in identifying and characterizing the S-locus genes that control the specificity of the selfincompatible (SI) reaction in the Rosaceae. The pistil and pollen determinants of S-specificity in the Rosaceae are a ribonuclease and an F-box protein, respectively. From allele-specific sequence polymorphism of S-specificity genes, molecular S-genotyping systems have been established in various rosaceous fruit tree species. Molecular characterization of S-determinants in self-compatible (SC) cultivars has revealed defects in the S-determinants that cause SC and enabled the development of molecular markers for SC. Marker-assisted selection of SC offspring is now being successfully incorporated into breeding programs. In this paper, we summarize the current knowledge of the molecular basis of self-(in)compatibility, S-genotyping methods, and molecular markers for SC in rosaceous fruit tree species.

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Section: Subgenus Prunofora (Japanese Apricot Apricot Andmentioning

confidence: 99%

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Yamane

Tao

2009

J. Japan. Soc. Hort. Sci.

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“…Entani et al (2003) identified S 9 haplotype from 'Bungo' at around the same time as Tao et al (2003) identified S 9 haplotype. Since it appeared that S 9 in Entani et al (2003) and Tao et al (2003) were different, Ikeda et al (2004) named the S 9 haplotype identified by Entani et al (2003) the S 9b haplotype. In this study, we use the nomenclature by Ikeda et al (2004).…”

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Cloning and Characterization of Twelve S-RNase Alleles in Japanese Apricot (Prunus mume Sieb. et Zucc.)

Habu¹,

Matsumoto²,

Fukuta³

et al. 2008

J. Japan. Soc. Hort. Sci.

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In this study, we cloned and sequenced PCR products from S-RNase alleles of 12 cultivars to determine their SRNase genotypes or S haplotypes. We identified a novel S 11 -RNase, which was previously misidentified as S 6 -RNase. In addition, the first and second intron sizes of S 1 -to S 11 -, and S f -RNases were presented. Since differences among the second intron sizes of S 3 -, S 9 -, and S 10 -RNases, as well as those among S 5 -, S 6 -, and S 11 -RNases, appeared to be very small, we developed primer sets for allele-specific PCR amplification to distinguish these S-RNase alleles. Consequently, 12 S-RNase alleles could be distinguished by PCR amplification using consensus primers for the second intron and/or the allele-specific primer sets. Using these PCR amplification, we determined SRNase genotypes of 14 cultivars, including 'Kagajizo' (S 6 S 10 ), 'Baigo' (S 6 S 10 ), and 'Orihime' (S 11 S f ), whose SRNase genotypes have been updated. The DNA sequence information for S-RNases and the allele-specific primer sets developed in this study would be useful for future S-RNase genotyping and thus S haplotyping in Japanese apricot.

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“…Previously, Tao et al (2000Tao et al ( , 2002 found that self-compatible cultivars of Japanese apricot share a common S-haplotype, termed S f -haplotpe, that can be used as a molecular marker for self-compatibility. Furthermore, to detect the self-compatible individuals easily, Tao et al (2003) developed a polymerase chain reaction (PCR) primer set, Ken2 and PM-R, from the intron sequence of S f -RNase, which can be used to specifically amplify the S f -RNase fragment. However, Ushijima et al (2004) recently found that an insertion into the SFB f could be a primary cause of the selfcompatibility in Japanese apricot cultivars with the S f -haplotype.…”

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A Simple and Rapid Procedure for the Detection of Self-Compatible Individuals in Japanese Apricot (Prunus mume Sieb. et Zucc.) Using the Loop-Mediated Isothermal Amplification (LAMP) Method

Habu¹,

Kishida²,

Morikita³

et al. 2006

HortSci

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Japanese apricot (Prunus mume Sieb. et Zucc.) exhibits S-RNase-based gametophytic self-incompatibility as do other Prunus species. Both self-incompatible and self-compatible Japanese apricot cultivars are grown commercially in Japan. These self-compatible cultivars are shown to have a common S-haplotype called S f that contains S f -RNase and SFB f (S-haplotype-specific F-box protein). This study describes a simple and rapid detection of SFB f , in Japanese apricot, based on loop-mediated isothermal amplification (LAMP) method. A set of 4 primers, F3, B3, FIP, and BIP primer, were designed from the exon and the putative inserted sequence of SFB f . Optimal reaction time at 63°C was determined to be 90 minutes. It appeared that the LAMP method combined with the ultrasimple DNA extraction efficiently detected SFB f . The advantage of the marker-assisted selection of self-compatibility based on the LAMP method was discussed.

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Development of the Sf-RNase Gene-Specific PCR Primer Set for Japanese Apricot (Prunus mume Sieb. et Zucc.)

Cited by 8 publications

References 8 publications

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Cloning and Characterization of Twelve S-RNase Alleles in Japanese Apricot (Prunus mume Sieb. et Zucc.)

A Simple and Rapid Procedure for the Detection of Self-Compatible Individuals in Japanese Apricot (Prunus mume Sieb. et Zucc.) Using the Loop-Mediated Isothermal Amplification (LAMP) Method

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