Development of the polymerase chain reaction for diagnosis of chancroid

Chui, Linda; Albritton, William L.; Paster, Bruce J.; Maclean, Ian; Marusyk, R. G.

doi:10.1128/jcm.31.3.659-664.1993

Cited by 36 publications

(13 citation statements)

References 33 publications

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“…The M-PCR assay for the detection of three etiologic agents of GUD described here provides for the simultaneous detection of the three agents from a single swab specimen. The multiplex PCR assay was at least as sensitive as that described in published reports of individual PCR assays for the three target organisms (4,7,11,16,37) and is more efficient and less prone to error compared with the individual assays.…”

Section: Discussionmentioning

confidence: 85%

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Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers

et al. 1996

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A multiplex PCR (M-PCR) assay with colorimetric detection was devised for the simultaneous amplification of DNA targets from Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus (HSV) types 1 and 2. By using target-specific oligonucleotides in a microwell format, 298 genital ulcer swab specimens collected in New Orleans during three intervals from 1992 through 1994 were evaluated. The results of the M-PCR assay were compared with the results of dark-field microscopy and H. ducreyi culture on two different culture media. HSV culture results were available for 99 specimens collected during the third interval. Confirmatory PCR assays targeting different gene sequences for each of the three organisms were used to validate the M-PCR results. Specimens were resolved as positive for the determination of sensitivity if the reference diagnostic test was positive or if the results of both the M-PCR and the confirmatory PCR were positive. The resolved sensitivities of M-PCR for HSV, H. ducreyi, and T. pallidum were 100, 98.4, and 91%, respectively. The resolved sensitivities of HSV culture, H. ducreyi culture, and dark-field microscopy were 71.8, 74.2, and 81%, respectively. These results indicate that the M-PCR assay is more sensitive than standard diagnostic tests for the detection of HSV, H. ducreyi, and T. pallidum from genital ulcers.

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Section: Discussionmentioning

confidence: 85%

“…The low detection limit of PCR offered a further advantage to culture by identifying HSV in crusted-over lesions, as well as before and after lesions appeared (7). H. ducreyi DNA has been detected in genital ulcer specimens by PCR, and PCR was more sensitive than culture (4,16).…”

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confidence: 99%

Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers

et al. 1996

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“…Two H. ducreyi probes have been developed by Chui et al (36), based on published sequences of the 16S rRNA gene of H. ducreyi (48,133). Although these probes were specific for H. ducreyi, neither reacted with all strains of H. ducreyi that were tested.…”

Section: Nonculture Diagnostic Testsmentioning

confidence: 99%

“…Three PCR assays for chancroid have been developed. Chui et al (36) used broad primers based on eubacterial 16S rRNA gene sequences to amplify a 303-bp sequence from members of the families Pasteurellaceae and Enterobacteriaceae. Using two H. ducreyispecific probes internal to this sequence, they obtained 100% sensitivity with 51 strains from six continents that were isolated over a 15-year period.…”

Section: Nonculture Diagnostic Testsmentioning

confidence: 99%

Chancroid and Haemophilus ducreyi: an update.

Trees

Morse

1995

Clinical Microbiology Reviews

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“…A variety of genetic probe (14,15) and amplification (16,17) methods have been developed for culture confirmation or direct detection of H ducreyi in clinical samples. Of particular relevance is the Roche-developed multiplex-PCR test (Roche Diagnostics Canada) that simultaneously detects H ducreyi, T pallidum and herpes simplex virus (17).…”

Section: Nucleic Acid Detection (With or Without Amplification)mentioning

confidence: 99%

The Laboratory Diagnosis of Haemophilus ducreyi

Alfa¹

2005

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Chancroid is a sexually transmitted infection caused by Haemophilus ducreyi. This fastidious, Gram-negative coccobacilli dies rapidly outside the human host, making diagnostic testing using culture methods difficult. This genital ulcer infection is not common in Canada and, therefore, can often be misdiagnosed. The objective of the present paper is to provide practical approaches for the diagnosis of chancroid in Canadian patients where the prevalence of this infection is low. Issues related to sample collection, sample transport and available diagnostic tests are reviewed, and several alternative approaches are outlined. Although antigen detection, serology and genetic amplification methods have all been reported for H ducreyi, none are commercially available. Culture is still the primary method available to most laboratories. However, the special media necessary for direct bedside inoculation is often not available; therefore, communication with the diagnostic laboratory and rapid specimen transport are essential when chancroid is suspected.

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Development of the polymerase chain reaction for diagnosis of chancroid

Cited by 36 publications

References 33 publications

Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers

Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers

Chancroid and Haemophilus ducreyi: an update.

The Laboratory Diagnosis of Haemophilus ducreyi

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