Development of the Myocardial Interstitium

Pogontke, Cristina; Guadix, Juan Antonio; Ruiz‐Villalba, Adrián; Pérez‐Pomares, José M.

doi:10.1002/ar.23915

Cited by 9 publications

(14 citation statements)

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“…Endothelial cells (ECs) in the coronary vascular system mainly originate from the sinus venosus and ventricular endocardium ( Red-Horse et al, 2010 ; Wu et al, 2012 ), with the contribution of septum transversum/epicardium ( Cano et al, 2016 ) and other extracardiac endothelial cells ( Palmquist-Gomes et al, 2018 ). The vast majority of cardiac ECs occupy the cardiac interstitium, a unique harboring compartment hosting a large variety of cells ( Pogontke et al, 2019 ). Cardiac fibroblasts (CFs) are prototypical interstitial cells, and most CFs derive from the embryonic epicardium ( Wessels et al, 2012 ; Moore-Morris et al, 2014 ; Ruiz-Villalba et al, 2015 ; Kanisicak et al, 2016 ), but endocardial-derived fibroblasts have also been described ( Zeisberg et al, 2007 ).…”

Section: Cellular Landscape Of the Adult Heart Before Single Cell Rna-seq Technologymentioning

confidence: 99%

“…Indeed, cardiac pumping activity requires the continuous interaction of a great variety of cell types, including contractile cardiomyocytes, endocardial cells, vascular endothelial and smooth muscle cells, cardiac fibroblasts and pacemaker cells, among many others. Cardiac cell interactions, mediated by juxtacrine, paracrine and endocrine signals, sustain cardiac homeostasis and are essential to articulate heart responses to pathologic stimuli ( Buckingham et al, 2005 ; Rog-Zielinska et al, 2016 ; Pogontke et al, 2019 ). These interactions are, at least in part, modulated by the specific functional profile of different cardiac chambers (e.g., intra-chamber pressure and resistance to blood flow are significantly different in the left versus the right ventricle).…”

Section: Introductionmentioning

confidence: 99%

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Understanding the Adult Mammalian Heart at Single-Cell RNA-Seq Resolution

Marín-Sedeño

Morentin

Pérez‐Pomares

et al. 2021

Front. Cell Dev. Biol.

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During the last decade, extensive efforts have been made to comprehend cardiac cell genetic and functional diversity. Such knowledge allows for the definition of the cardiac cellular interactome as a reasonable strategy to increase our understanding of the normal and pathologic heart. Previous experimental approaches including cell lineage tracing, flow cytometry, and bulk RNA-Seq have often tackled the analysis of cardiac cell diversity as based on the assumption that cell types can be identified by the expression of a single gene. More recently, however, the emergence of single-cell RNA-Seq technology has led us to explore the diversity of individual cells, enabling the cardiovascular research community to redefine cardiac cell subpopulations and identify relevant ones, and even novel cell types, through their cell-specific transcriptomic signatures in an unbiased manner. These findings are changing our understanding of cell composition and in consequence the identification of potential therapeutic targets for different cardiac diseases. In this review, we provide an overview of the continuously changing cardiac cellular landscape, traveling from the pre-single-cell RNA-Seq times to the single cell-RNA-Seq revolution, and discuss the utilities and limitations of this technology.

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Section: Cellular Landscape Of the Adult Heart Before Single Cell Rna-seq Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Understanding the Adult Mammalian Heart at Single-Cell RNA-Seq Resolution

Marín-Sedeño

Morentin

Pérez‐Pomares

et al. 2021

Front. Cell Dev. Biol.

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“…Around 70% of these cells reside in the cardiac interstitium (CI), the extracellular space between cardiomyocytes ( Bergmann et al, 2015 ; Pinto et al, 2016 ). All these cells are jointly referred to as cardiac interstitial cells (CICs) and are known to play an essential role in myocardial embryonic development, and adult homeostasis ( Krenning et al, 2010 ; Pérez-Pomares and de La Pompa, 2011 ; Pogontke et al, 2019 ), and in the adaptive responses of the heart to pathological conditions ( Takeda et al, 2011 ; Ruiz-Villalba et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…This finding is in accordance with extensive myocardial vascularization by coronary vessels, including arteries, veins, and a massive capillary bed ( Tomanek et al, 2010 ). Furthermore, the coronary endothelium has a key scaffolding role during embryonic interstitium formation ( Pogontke et al, 2019 ) since coronary EC interaction with vSMCs, pericytes, and CFs is necessary for the building of periendothelial coronary domains. Moreover, due to the cellular complexity, a niche role for the periendothelial coronary milieu has been proposed ( Fioret et al, 2014 ; Pogontke et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamic Epicardial Contribution to Cardiac Interstitial c-Kit and Sca1 Cellular Fractions

Pogontke

Guadix

Sánchez-Tévar

et al. 2022

Front. Cell Dev. Biol.

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Background: The cardiac interstitial cellular fraction is composed of multiple cell types. Some of these cells are known to express some well-known stem cell markers such as c-Kit and Sca1, but they are no longer accepted to be true cardiac stem cells. Although their existence in the cardiac interstitium has not been disputed, their dynamic throughout development, specific embryonic origin, and potential heterogeneity remain unknown. In this study, we hypothesized that both c-KitPOS and Sca1POS cardiac interstitial cell (CIC) subpopulations are related to the Wilms’ tumor 1 (Wt1) epicardial lineage.Methods: In this study, we have used genetic cell lineage tracing methods, immunohistochemistry, and FACS techniques to characterize cardiac c-KitPOS and Sca1POS cells.Results: Our data show that approximately 50% of cardiac c-KitPOS cells are derived from the Wt1-lineage at E15.5. This subpopulation decreased along with embryonic development, disappearing from P7 onwards. We found that a large proportion of cardiac c-KitPOS cells express specific markers strongly suggesting they are blood-borne cells. On the contrary, the percentage of Sca1POS cells within the Wt1-lineage increases postnatally. In accordance with these findings, 90% of adult epicardial-derived endothelial cells and 60% of mEFSK4POS cardiac fibroblasts expressed Sca1.Conclusion: Our study revealed a minor contribution of the Wt1-epicardial lineage to c-KitPOS CIC from embryonic stages to adulthood. Remarkably, a major part of the adult epicardial-derived cell fraction is enriched in Sca1, suggesting that this subpopulation of CICs is heterogeneous from their embryonic origin. The study of this heterogeneity can be instrumental to the development of diagnostic and prognostic tests for the evaluation of cardiac homeostasis and cardiac interstitium response to pathologic stimuli.

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“…In a contribution from the lab of José Maria Perez‐Pomares, Cristina Pogontke, a graduate student, and co‐first author Juan Guadix, a postdoctoral fellow, elegantly describe the changing cellular and molecular characteristics of the cardiac interstitium throughout development. Furthermore, the authors discuss the role of components of the cardiac interstitium during ischemia and non‐ischemic pathological conditions showing the important role this space is playing in the healthy heart and in the heart under pathological conditions (Pogontke et al, ).…”

Section: Introductionmentioning

confidence: 99%

The State of Cardiovascular Developmental Biology is Strong — Honoring Dr. Roger Markwald and his Seminal Contributions to the Field

Wessels

2018

The Anatomical Record

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In August 2017, the Cardiovascular Developmental Biology Center (CDBC), together with the “Department of Regenerative Medicine and Cell Biology (RMCB) at the Medical University of South Carolina (MUSC), organized their 13th Annual CDBC Symposium. During this special event, which was organized in collaboration with The Anatomical Record, the unique and important contributions of Dr. Roger Markwald (known to all of us as Roger) to the field of cardiovascular research were celebrated. Fifteen leading investigators in the field presented their ideas and reported results of their studies to an audience that included many familiar faces from Roger's past and present. This group consisted of established investigators from around the world as well as young and upcoming scientists from local institutions. In their presentations, the platform speakers emphasized the significance of Roger's scientific contributions and advice to their professional development and career. In this Special Issue of The Anatomical Record, we assembled a collection of invited papers written by several attendees of the symposium. The issue also contains a number of articles written by colleagues who, for one reason or the other, were not able to attend the meeting, but expressed their desire to contribute to this special “festschrift” of The Anatomical Record in honor and recognition of Roger's amazing career. Anat Rec, 302:14–18, 2019. © 2018 Wiley Periodicals, Inc.

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Development of the Myocardial Interstitium

Cited by 9 publications

References 146 publications

Understanding the Adult Mammalian Heart at Single-Cell RNA-Seq Resolution

Understanding the Adult Mammalian Heart at Single-Cell RNA-Seq Resolution

Dynamic Epicardial Contribution to Cardiac Interstitial c-Kit and Sca1 Cellular Fractions

The State of Cardiovascular Developmental Biology is Strong — Honoring Dr. Roger Markwald and his Seminal Contributions to the Field

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