Abstract:To develop a method for isolation and purification of recombinant human interleukin-7 from bacterial inclusion bodies. Methods. Protein synthesis via fermentation, densitometry, refolding method of gel filtration, two-stage chromatographic purification of the protein.Results. The inclusion bodies obtained via fermentation in E.coli cells contained recombinant human interleukin-7, which was solubilized at 22ºС for 1 hour in the following buffer solution: 7M of guanidine hydrochloride, 100mM of Tris-НСl, 0.1 % T… Show more
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