Development of the H3N2 influenza microneedle vaccine for cross-protection against antigenic variants

Shin, Yura; Kim, Jeonghun; Seok, Jong Hyeon; Park, Heedo; Cha, Hye‐Ran; Ko, Si Hwan; Lee, Jae Myun; Park, Man‐Seong; Park, Jung Hwan

doi:10.1038/s41598-022-16365-2

Cited by 8 publications

(4 citation statements)

References 46 publications

(30 reference statements)

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“…Further, the arrays can be fabricated on-site without the need for trained healthcare personnel. Another work by Shin et al 376 demonstrates the utility of HPLC in the coating process to produce H3N2 influenza vaccinations that perform comparably to traditional intramuscular vaccines. Together, these works present advancement in multiple areas of vaccine technology and supports the future success of microneedle-based vaccination.…”

Section: Local Immunomodulationmentioning

confidence: 99%

Therapeutic synthetic and natural materials for immunoengineering

Slezak,

Chang,

Hossainy

et al. 2024

Chem. Soc. Rev.

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Section: Local Immunomodulationmentioning

confidence: 99%

Therapeutic synthetic and natural materials for immunoengineering

Slezak,

Chang,

Hossainy

et al. 2024

Chem. Soc. Rev.

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“…These strategies include the accurate administration of antigenic material, control over release patterns, and well-informed design based on a more profound comprehension of immune system mechanisms and pathogen-host interactions [66]. One study employed an H3N2 microneedle vaccine, which produced a cross-protective immune response against many H3N2 antigenic variants [67]. Alternate approaches encompass employing conserved antigens like HA, NA, matrix, and internal proteins, coupled with diverse vaccine platforms such as recombinant antigen/protein-based, virus-vectored, nanoparticle-based, DNA/RNA-based, virus-like particle (VLP), and multiplex vaccines [68][69][70].…”

Section: Novel Approaches To Vaccine Designmentioning

confidence: 99%

Targeting H3N2 Influenza: Advancements in Treatment and Vaccine Strategies

Srivastava,

Jayaswal,

Kumar

et al. 2024

Preprint

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The emergence of the H3N2 influenza virus in 1968 marked a significant event as it crossed the species barrier. Since then, ongoing mutational dynamics have led to the formation of antigenic clusters, prompting the World Health Organization to advocate for regular updates to H3N2 vaccines. Research in the Western Pacific region underscores the necessity for heightened awareness and effective control strategies. Stemming from avian influenza A, the 1968 H3N2 influenza pandemic resulted in the deaths of one million people globally, with its seasonal variants primarily affecting older individuals and causing severe illness due to antigenic drift. To address the challenge of vaccine efficacy against H3N2 mutations, researchers are exploring innovative strategies such as precise antigenic material administration, controlled release patterns, understanding immune system mechanisms, and glycan engineering. This review comprehensively examines various aspects of the Influenza A (H3N2) virus, encompassing its virological characteristics, evolutionary trends, global epidemiology, vaccination strategies, antiviral interventions, and emerging diagnostic approaches. It underscores the impact of antigenic variation on vaccine design and effectiveness, seasonal outbreak patterns, pandemic potential, and the interplay between viral factors and host immune responses. Moreover, the review evaluates antiviral therapies and the issue of drug resistance, emphasising the necessity for multidisciplinary approaches involving researchers, healthcare professionals, and policymakers to comprehend H3N2 and enhance public health interventions.

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“…Seasonal influenza viruses cause local epidemics as well as unpredictable periodic pandemics. Therefore, it is crucial to identify seasonal viruses at an early stage to determine their prevalence in a year. , In particular, seasonal influenza A virus (IAV) H3N2 has been reported as a novel reassortant annually, exhibiting rapid genetic and antigenic evolution in an attempt to escape the pressures of the host’s immunity. − This makes it more challenging to characterize the virus. Recently, H3N2 has been cocirculating in humans with H1N1 and influenza B virus.…”

Section: Introductionmentioning

confidence: 99%

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

Kim,

Jeong,

Hwang

et al. 2023

ACS Appl. Mater. Interfaces

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Influenza viruses are known to cause pandemic flu outbreaks through both inter-human and animal-to-human transmissions. Therefore, the rapid and accurate detection of such pathogenic viruses is crucial for effective pandemic control. Here, we introduce a novel sensor based on affinity peptide-immobilized hydrogel microspheres for the selective detection of influenza A virus (IAV) H3N2. To enhance the binding affinity performance, we identified novel affinity peptides using phage display and further optimized their design. The functional hydrogel microspheres were constructed using the drop microfluidic technique, employing a structure composed of natural (chitosan) and synthetic (poly(ethylene glycol) diacrylate and PEG 6 kDa) polymers with the activation of azadibenzocyclooctyne for the subsequent click chemistry reaction. The binding peptide-immobilized hydrogel microsphere (BP-Hyd) was characterized by field emission scanning electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy and exhibited selective detection capability for the IAV H3N2. To capture the detected IAV H3N2, a Cy3-labeled IAV hemagglutinin antibody was utilized. By incorporating the affinity peptide with hydrogel microspheres, we achieved quantitative and selective detection of IAV H3N2 with a detection limit of 1.887 PFU mL–1. Furthermore, the developed suspension sensor exhibited excellent reproducibility and showed reusability potential. Our results revealed that the BP-Hyd-based fluorescence sensor platform could be feasibly employed to detect other pathogens because the virus-binding peptides can be easily replaced with other peptides through phage display, enabling selective and sensitive binding to different targets.

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Development of the H3N2 influenza microneedle vaccine for cross-protection against antigenic variants

Cited by 8 publications

References 46 publications

Therapeutic synthetic and natural materials for immunoengineering

Therapeutic synthetic and natural materials for immunoengineering

Targeting H3N2 Influenza: Advancements in Treatment and Vaccine Strategies

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

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