Development of the gene-based SCARs for the Ph-3 locus, which confers late blight resistance in tomato

Park, Young-Hoon; Hwang, Jang Yeon; Kim, Kwang-Hwan; Kang, Jum-Soon; Kim, Byung-Sup; Xu, Shixiao; Ahn, Young-Sup

doi:10.1016/j.scienta.2013.08.013

Cited by 23 publications

(24 citation statements)

References 22 publications

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“…However, field-grown tomatoes are susceptible to disease such as late blight (LB), which is one of the most devastating diseases of field-grown tomatoes, and it can cause severe economic losses for tomato growers242526. Late blight is caused by the pathogen Phytophthora infestans .…”

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confidence: 99%

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

Luan

Cui

Wang

et al. 2016

Sci Rep

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Late blight of tomato is caused by the oomycete pathogen Phytophthora infestans. In our previous work, we identified and characterized a miR1918 in P. infestans (pi-miR1918), and showed that its sequence is similar to the sequence of tomato miR1918 (sly-miR1918). In this study, we used Arabidopsis thaliana pre-miR159a as a backbone to synthesize pi-miR1918 via PCR and mutagenesis. The artificial pi-miR1918 was used to investigate the role of miR1918 in tomato-P. infestans interaction. Trangenic tomato plants that overexpressed the artificial pi-miR1918 displayed more serious disease symptoms than wild-type tomato plants after infection with P. infestans, as shown by increased number of necrotic cells, lesion sizes and number of sporangia per leaf. The target genes of pi-miR1918 and sly-miR1918 were also predicted for tomato and P. infestans, respectively. qPCR analysis of these targets also performed during tomato-P. infestans interaction. The expression of target gene, RING finger were negatively correlated with miR1918 in the all Lines of transgenic tomato plants. In addition, we used the 5′ RACE to determine the cleavage site of miR1918 to RING finger. These results suggested that miR1918 might be involved in the silencing of target genes, thereby enhancing the susceptibility of tomato to P. infestans infection.

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confidence: 99%

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

Luan

Cui

Wang

et al. 2016

Sci Rep

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“… The Ph‐3 on‐gene SCAR marker developed by Park et al. (). A non‐parental susceptible pattern (S′) was observed. …”

Section: Resultsmentioning

confidence: 99%

“…), SCAR‐Ph3‐3 (SCAR; Park et al. ) and TG328/ Bst NI (CAPS; Robbins et al. ), were used to genotype the RIL population.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

et al. 2016

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Late blight caused by Phytophthora infestans is one of the most destructive diseases of tomato (Solanum lycopersicum L.) that mainly occurs in cool and wet environments. With the spread of the A2 mating type and new clonal lineages, fewer fungicides provide effective control of the disease, which has increased its worldwide threat. Host resistance could contribute significantly to sustainable disease control. Ph-3 is a race-specific late blight resistance gene commonly used in commercial tomato breeding. Availability of precise and easy to use gene-based markers would facilitate selection. In this study, a Ph-3 on-gene cleaved amplified polymorphic sequence (CAPS) marker, Ph3.gsm/HincII, was developed based on the published gene sequence of Ph-3. The effectiveness of the marker was evaluated along with other published Ph-3 markers using an F 9 recombinant inbred line (RIL) population derived from NC 23E-2 (93) 9 L3708. Markers Ph3.gsm/HincII and TG328/BstNI accurately genotyped the RIL population for Ph-3. In addition, Ph3.gsm/HincII was able to differentiate variable susceptible alleles. This reliable codominant DNA marker would be very useful in marker-assisted selection, particularly for resistance gene pyramiding.

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“…As a reference array of accessions for marker validation, 64 SolCAP germplasms for which genome-wide SNP genotypes are available from DB were collected and evaluated for their fruit peel color. Genomic DNA extraction and purification was conducted using true leaves according to the method described by Park et al (2013).…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

Kim¹,

Kim²,

Kang³

et al. 2015

HST

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Yellow or transparent fruit peel color is caused by the accumulation or lack of naringenin chalcone (NG, C) in fruit peel and determines the red or pink appearance of tomato fruit, respectively. NGC biosynthesis is regulated by the SlMYB12 gene of the Y locus on chromosome 1, and DNA markers derived from SlMYB12 would be useful for marker-assisted selection (MAS) of tomato fruit color. To develop a gene-based marker, 4.9 kb of the SlMYB12 gene including a potential promoter region was sequenced from the red-fruited (YY) line 'FCR' and pink-fruited (yy) line 'FCP'. Sequence alignment of these SlMYB12 alleles revealed no sequence variations between 'FCR' and 'FCP'. To identify SlMYB12-linked single nucleotide polymorphisms (SNPs), 'FCR' and 'FCP' were genotyped using a SolCAP Tomato SNP array and CAPS markers (CAPS-456, 531, 13762, and 38123) were developed from the four SNPs (solcap_snp_sl_456, 531, 13762, and 38123) most closely flanking the SlMYB12. These CAPS markers were mapped using F2 plants derived from 'FCR' × 'FCP'. The map positions of the fruit peel color locus (Y) were CAPS-13762 (0 cM) -456 (11.09 cM) -Y (15.71 cM) -38123 (17.82 cM) -531 (30.86 cM), and the DNA sequence of SlMYB12 was physically anchored in the middle of CAPS-456 and CAPS-38123, indicating that fruit peel color in domesticated tomato is controlled by SlMYB12. A total of 64 SolCAP tomato germplasms were evaluated for their fruit peel color and SNPs located between solcap_snp_sl_456 and 38123. Seven SNPs that were detected in this interval were highly conserved for pink-fruited accessions and specific to transparent fruit peel traits, as depicted by a phenetic tree of 64 accessions based on the seven SNPs.

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Development of the gene-based SCARs for the Ph-3 locus, which confers late blight resistance in tomato

Cited by 23 publications

References 22 publications

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

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