Development of the engineered “glanded plant and glandless seed” cotton

Gao, Wenhao; Zhu, Xiefei; Lee, Ding; Xu, Biyu; Gao, Yang; Cheng, Yung Ming; Liu, Bingliang; Si, Zhanfeng; Fang, Lei; Guan, Xueying; Zhu, Shuijin; Zhang, Tianzhen; Hu, Yan

doi:10.1016/j.fochms.2022.100130

Cited by 10 publications

(8 citation statements)

References 50 publications

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“…(Figure 2B), consistent with the presence of glands (Ma et al, 2016). GoPGF was proven to be specific to gland development and had no noticeable effect on plant growth and development (Gao et al, 2022). The silencing of GoPGF can lead to a complete glandless phenotype.…”

Section: Resultssupporting

confidence: 78%

Visible gland constantly traces virus-induced gene silencing in cotton

Tian

et al. 2022

Front. Plant Sci.

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A virus-induced gene silencing (VIGS) system was established to induce endogenous target gene silencing by post-transcriptional gene silencing (PTGS), which is a powerful tool for gene function analysis in plants. Compared with stable transgenic plant via Agrobacterium-mediated gene transformation, phenotypes after gene knockdown can be obtained rapidly, effectively, and high-throughput through VIGS system. This approach has been successfully applied to explore unknown gene functions involved in plant growth and development, physiological metabolism, and biotic and abiotic stresses in various plants. In this system, GhCLA1 was used as a general control, however, silencing of this gene leads to leaf albino, wilting, and plant death ultimately. As such, it cannot indicate the efficiency of target gene silencing throughout the whole plant growth period. To address this question, in this study, we developed a novel marker gene, Gossypium PIGMENT GLAND FORMATION GENE (GoPGF), as the control to trace the efficiency of gene silencing in the infected tissues. GoPGF has been proved a key gene in gland forming. Suppression of GoPGF does not affect the normal growth and development of cotton. The number of gland altered related to the expression level of GoPGF gene. So it is a good marker that be used to trace the whole growth stages of plant. Moreover, we further developed a method of friction inoculation to enhance and extend the efficiency of VIGS, which facilitates the analysis of gene function in both the vegetative stage and reproductive stage. This improved VIGS technology will be a powerful tool for the rapid functional identification of unknown genes in genomes.

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Section: Resultssupporting

confidence: 78%

Visible gland constantly traces virus-induced gene silencing in cotton

Tian

et al. 2022

Front. Plant Sci.

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“…Developing edible cottonseed food with several strategies of degossypolization. resistance of plants to pests, with non-significant change in the levels of seed protein, oil content, fiber yield and quality (Gao et al, 2022). Moreover, the MYB transcription factor CGP1 participates in gossypol accumulation through the formation of heterodimers with GoPGF, and silencing CGP1 can also lead to a sharp reduction in gossypol levels (Gao et al, 2020).…”

Section: Detoxifying Through Conventional and Molecular Breedingmentioning

confidence: 99%

“…At the same time, ultra-low gossypol cottonseed (ULGCS) variety showed no deleterious effects on fiber/seed yield, quality or other agronomic performance, and its stability has been confirmed by multi-year and multi-site field trials ( Rathore et al., 2020 ). Similarly, specific silencing of the gland formation gene GoPGF in seeds decreased gossypol content by 98% and maintained normal resistance of plants to pests, with non-significant change in the levels of seed protein, oil content, fiber yield and quality ( Gao et al., 2022 ). Moreover, the MYB transcription factor CGP1 participates in gossypol accumulation through the formation of heterodimers with GoPGF, and silencing CGP1 can also lead to a sharp reduction in gossypol levels ( Gao et al., 2020 ).…”

Section: Detoxifying Through Conventional and Molecular Breedingmentioning

confidence: 99%

Multiple strategies to detoxify cottonseed as human food source

Liu

Zhai

et al. 2022

Front. Plant Sci.

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“…Traditional cottonseed is not suitable for human consumption because of the presence of a toxic ingredient (i.e., gossypol) in its pigment glands [ 1 , 2 ]. Glandless (Gl) cotton is a new variety with gossypol content in its cottonseed <450 mg kg −1 dry mass to meet the US FDA’s criteria of gossypol limit as human food [ 3 , 4 ]. Thus, exploration of these Gl cottonseeds as food products would greatly enhance the economic impacts of these new cotton lines [ 3 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stability of Cottonseed Butter Products under Accelerated Storage Conditions

Nam

Klasson

2023

Molecules

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Cottonseed is a natural product of cotton (Gossypium spp.) crops. This work evaluated the oxidative stability of cottonseed butters through accelerated autoxidation by storage at 60 °C for 25 days. Three oxidative stability parameter values (peroxide value, p-anisidine value, and total oxidation value) were monitored over the storage time. These chemical measurements revealed that the storage stability of the butter products was dominated by primary oxidation of lipid (oil) components, while the secondary oxidation levels were relatively unchanged over the storage time. An analysis of the tocopherols (natural oxidants in cottonseed) suggested not only the protection function of the molecules against oxidation of the cottonseed butter during storage, but also the dynamic mechanism against the primary oxidation of lipid components. Attenuated total reflectance–Fourier-transform infrared spectroscopy (ATR–FTIR) data confirmed no changes in the major C functional groups of cottonseed butters over the storage time. On the other hand, characteristic minor peaks of conjugated dienes and trienes related to lipid oxidation were impacted by the accelerated storage. As each day of accelerated oxidation at 60 °C is equivalent to 16 days of storage at 20 °C, observations in this work should have reflected the oxidative stability behaviors of the cottonseed butters after about 13 months of shelf storage under ambient storage conditions. Thus, these data that were collected under the accelerated oxidation testing would be useful not only to create a better understanding of the autooxidation mechanism of lipid molecules in cottonseed butters, but also in developing or recommending appropriate storage conditions for cottonseed end products to prevent them from quality degradation.

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Development of the engineered “glanded plant and glandless seed” cotton

Cited by 10 publications

References 50 publications

Visible gland constantly traces virus-induced gene silencing in cotton

Visible gland constantly traces virus-induced gene silencing in cotton

Multiple strategies to detoxify cottonseed as human food source

Oxidative Stability of Cottonseed Butter Products under Accelerated Storage Conditions

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