Development of the 33‐, 23‐ and 16‐kDa polypeptides of the photosynthetic oxygen‐evolving system during greening

Ryrie, Ivan J.; Young, San-Lin; Andersson, Bertil

doi:10.1016/0014-5793(84)81297-1

Cited by 19 publications

(7 citation statements)

References 19 publications

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“…We do not know whether this is due to the low amount of the 16kDa polypeptide (Ryrie et al 1984) present in the latter case and its gradual accumulation or to the gradual photoactivation of the water-splitting activity which may take place (Michel and Sironval 1972) as the number of the two-minutes flashes is increased. It should be noted, however, that parallel to the appearance of the water-splitting capacity (Table 3), a marked and synchronous accumulation of the 33, 23, and 18 kDa polypeptides, propably originating in the water-splitting complex, is observed (see polypeptides in Fig.…”

Section: Discussionmentioning

confidence: 97%

The effect of the dark interval in intermittent light on thylakoid development: photosynthetic unit formation and light harvesting protein accumulation

Tzinas

Argyroudi‐Akoyunoglou

Akoyunoglou

1987

Photosynth Res

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Etiolated bean plants were grown in intermittent light with dark intervals of shorter or longer duration, to modulate the rate of chlorophyll accumulation, relative to that of the other thylakoid components formed. We thus produced conditions under which chlorophyll becomes more or less a limiting factor. We then tested whether LHC complexes can be incorporated in the thylakoid. It was found that an equal amount of chlorophyll, formed under the same total irradiation received, may be used for the stabilization of few and large-in-size PS units containing LHC components (short dark-interval intermittent light), or for the stabilization of many and small-in-size PS units with no LHC components (long dark-interval intermittent light). The size of the PS units diminishes as the dark-interval duration is increased, with no further change after 98 minutes. The PSII/cytf ratio remains constant throughout development in intermittent light and equal to that of mature chloroplasts (PSII/cytf = 1) except in the case of very long dark-interval regimes, where about half PSII units per cytf are present. The PSII/PSI ratio was found to be correlated with the PSII unit size (the larger the size, the lower the ratio). The number of PSI units operating on the same electron transfer chain varied depending on the size of the PSII unit (the larger the PSII unit size, the more the PSI units per chain). The results suggest that it is not the chlorophyll content per se which regulates the stabilization of LHC in developing thylakoids and consequently the size of the PS units, but rather the rate by which it is accumulated, relative to that of the other thylakoid components.

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Section: Discussionmentioning

confidence: 97%

The effect of the dark interval in intermittent light on thylakoid development: photosynthetic unit formation and light harvesting protein accumulation

Tzinas

Argyroudi‐Akoyunoglou

Akoyunoglou

1987

Photosynth Res

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“…Some of these proteins, e.g. OEC33, OEC23, OEC17 and Hcf136, are essential for the correct assembly of the photosystems and are therefore needed at an early stage of chloroplast development (Hashimoto et al 1993, Ryrie et al 1984). We identified OEC33 and OEC23 but not OEC17 in our EPIM preparation.…”

Section: Discussionmentioning

confidence: 99%

“…Later studies showed that, besides POR, the α‐ and β‐subunits of chloroplast coupling factor 1 (CF 1 ) constitute the main part of the EPIM proteome (Lütz et al 1981, Ryberg and Sundqvist 1982, Santel and Apel 1981), while the δ‐, ɛ‐ and γ‐subunits of CF 1 , as well as subunits I and II of CF 0 , were found to a minor extent (Høyer Hansen et al 1979, Selstam et al 1989). Cytochromes, plastocyanin, ferredoxin NADPH reductase (FNR), ferredoxin, Rieske Fe–S centre, D2, photosystem II (PSII) stability/assembly factor (Hcf136) and the 23‐kDa and 33‐kDa oxygen‐evolving complex proteins (OEC23 and OEC33) were shown to be present in etioplasts of various angiosperms (Hashimoto et al 1993, Haslett and Cammack 1976, Høyer Hansen 1980, Meurer et al 1998, Müller and Eichacker 1999, Ryrie et al 1984, Takabe et al 1986). In contrast to our knowledge of the final protein composition, i.e.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the etioplast inner membranes of wheat (Triticum aestivum) by two‐dimensional electrophoresis and mass spectrometry

Blomqvist

Ryberg

Sundqvist

2006

Physiologia Plantarum

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The proteome of the etioplast inner membranes (EPIM) of dark-grown wheat leaves (Triticum aestivum L.) was mapped as an essential part of studies on plastid differentiation. Proteins were separated by two-dimensional gel electrophoresis and analysed with mass spectrometry (MS). Over 200 protein spots were resolved and visualized by Coomassie blue staining. More than 100 spots were submitted for subsequent mass spectrometry analyses by matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) MS, electrospray tandem MS (ESI-MS/MS) or liquid chromatography-mass spectrometry (LC-MS/MS). There were 46 identified spots, from which at least 21 different proteins were identified. Among these were FtsH proteases and the peptidyl-prolyl cis-trans isomerase TLP40, as well as chloroplast coupling factor subunits and extrinsic subunits of photosystem II (PSII). Of special interest is the NADPH:protochlorophyllide oxidoreductase (POR), which is the predominant protein of prolamellar bodies, where it accumulates in a highly stable ternary complex with protochlorophyllide and NADPH. This complex is known to play an important role in the formation and dispersal of prolamellar bodies. Five different isoforms of POR, with different pI values, were identified. We discuss the possibility of these isoforms being differently phosphorylated as part of the regulation of POR-pigment complexes. The proteome mapping of EPIM is a crucial step in the understanding of the lightdependent transition of etioplasts to chloroplasts, and provides a basis for functional studies on factors influencing the greening process.

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“…Some of the proteins in the photosynthetic machinery, e.g. Hcf136 and the oxygenevolving complex, are of importance already at an early stage of chloroplast development, because of their roles in the correct assembly of the photosystems (Ryrie et al 1984;Hashimoto et al 1993;Müller and Eichacker 1999;Plücken et al 2002). The presence of chlorophyll is a prerequisite for the accumulation of most chlorophyll-binding proteins, e.g.…”

Section: Thylakoid Proteins As Components Of Plbsmentioning

confidence: 98%

Proteomic analysis of highly purified prolamellar bodies reveals their significance in chloroplast development

2007

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The prolamellar body (PLB) proteome of dark-grown wheat leaves was characterized. PLBs are formed not only in etioplasts but also in chloroplasts in young developing leaves during the night, yet their function is not fully understood. Highly purified PLBs were prepared from 7-day-old dark-grown leaves and identified by their spectral properties as revealed by low-temperature fluorescence spectroscopy. The PLB preparation had no contamination of extra-plastidal proteins, and only two envelope proteins were found. The PLB proteome was analysed by a combination of 1-D SDS-PAGE and nano-LC FTICR MS. The identification of chlorophyll synthase in the PLB fraction is the first time this enzyme protein was found in extracts of dark-grown plants. This finding is in agreement with its previous localization to PLBs using activity studies. NADPH:protochlorophyllide oxidoreductase A (PORA), which catalyses the reduction of protochlorophyllide to chlorophyllide, dominates the proteome of PLBs. Besides the identification of the PORA protein, the PORB protein was identified for the first time in dark-grown wheat. Altogether 64 unique proteins, representing pigment biosynthesis, photosynthetic light reaction, Calvin cycle proteins, chaperones and protein synthesis, were identified. The in number of proteins' largest group was the one involved in photosynthetic light reactions. This fact strengthens the assumption that the PLB membranes are precursors to the thylakoids and used for the formation of the photosynthetic membranes during greening. The present work is important to enhance our understanding of the significance of PLBs in chloroplast development.

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Development of the 33‐, 23‐ and 16‐kDa polypeptides of the photosynthetic oxygen‐evolving system during greening

Cited by 19 publications

References 19 publications

The effect of the dark interval in intermittent light on thylakoid development: photosynthetic unit formation and light harvesting protein accumulation

The effect of the dark interval in intermittent light on thylakoid development: photosynthetic unit formation and light harvesting protein accumulation

Proteomic analysis of the etioplast inner membranes of wheat (Triticum aestivum) by two‐dimensional electrophoresis and mass spectrometry

Proteomic analysis of highly purified prolamellar bodies reveals their significance in chloroplast development

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