Development of tetracycline-serratiopeptidase-containing periodontal gel: Formulation and preliminary clinical study

Abstract

The purpose of this research was to reduce the polymer concentration and to obtain reasonable viscosity at a lower concentration of pluronic by the addition of a viscosity modifier. A 20% wt/wt pluronic gel was prepared on a weight basis using the cold method. The effect of the amount of tetracycline and Aerosil on gel properties was studied. The gel was evaluated using different parameters: polarizing microscopy, gelation, gel melting, bioadhesivity, viscosity, drug release, and stability of enzyme. An in vivo study was performed to evaluate the clinical efficiency of the liquid crystalline gel. Addition of Aerosil to the gel favored hexagonal phase formation. Viscosity and bioadhesivity increased with an increase in the concentration of Aerosil. Release of tetracycline was sustained as the concentration of Aerosil increased. Various clinical parameters confirmed the acceptability and efficiency of this gel system.

The effectiveness of 0.5–0.7% tetracycline gel to reduced subgingival plaque bacteria

“…Thus, the tetracycline gel formulation along with with scaling root and planning was effective in reducing gingival inflammation, bleeding on probing, pocket depth and increasing clinical attachment. 8 In summary, our result suggest that tetracycline gel with 0,5% concentration is effective in inhibiting the growth of subgingival plaque bacteria. …”

“…13 By applicating tetracycline as gel, it can be absorbed into the gingival connective tissue and penetrate the root surface, therefore increasing the antibacterial capacity towards periodontopathogenic bacteria. 8 In addition, Tetracycline also inhibits the activity of matrix metalloproteinase produced by PMN (MMP-8 and MMP-9) by binding Ca 2+ and Zn 2+ which is located at its active side. So it inhibits the resorption of alveolar bones and collagen degeneration.…”

“…7 Tetracycline gel could be effective in the pocket for 48 hours to 14 days. 8 Hydrophilic gel is one of semisolid prepares with some advantages such easily and quickly applied, unsticky and comfort. 9 Currently, metronidazole gel 25% is sold in Indonesia is metrodanizole gel 25%.…”

Perioceutics in the management of Periodontal Disease

“…20 Periodontal gel formulations containing tetracyclineserratiopeptidase has shown better results along with non surgical therapy. 21 In summary, the studies reported that tetracycline fibers used as a monotherapy without non surgical therapy were effective at decreasing probing depths, in gaining clinical attachment and reducing bacterial load.…”

Local Drug Delivery in the Treatment of Periodontitis: A Review.

“…In a 6-month multi-centre evaluation of adjunctive tetracycline fibre therapy by Newman et al 1994, showed that fibre therapy significantly enhanced the effectiveness of scaling and root planing in the management of localized recurrent periodontitis sites, in patients receiving regular supportive periodontal therapy 7 . A Tetracycline-SerratiopeptidaseContaining Periodontal Gel made by Maheshwari et al observed that Formulation has shown statistically significant results along with scaling and root planing 8 . Singh et al 2009 reported that there is no statically significant difference in the results achieved with local tetracycline hydrochloride or local metronidazole as adjuncts to mechanotherapy 9 .…”