Development of Test System for Detection of V617F Mutation of JAK2 Gene in Patients with Chronic Myeloproliferative Disorders

Development of molecular oncohematology in Ukraine

“…Under the current classification of the World Health Organization (4.5) molecular diagnostics is an obligatory component of modern diagnostics of leukemias. In collaboration with the colleagues from the Immunocytochemistry department of Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine) we have developed a protocol for detection of the most characteristic MPN mutations, namely Jak2, mpl, tet2, asxl1 [30], and mutations in acute leukemia mll/af4 (t (4, 11) (q21; q23); mll/af9 (t (9; 11) (p22; q23); cbfb/myh11 (inv16) [31]. The effectiveness of this protocol was tested on a large number of patients.…”