Development of ten microsatellite markers for Alibertia edulis (Rubiaceae), a Brazilian savanna tree species

Abstract

Ten microsatellite markers were developed using next-generation sequencing data for Alibertia edulis (Rubiaceae), a widely distributed species typical of Cerrado (Brazilian savanna) vegetation. The markers were polymorphic in the two populations analyzed. The numbers of alleles, and observed (H O ) and expected (H E ) heterozygosities per polymorphic locus ranged from 2 to 11, 0.091 to 1.0, and 0.100 to 0.937 respectively. The SSR loci demonstrated moderate to high polymorphism values in both populations analyzed, with PIC values ranging from 0.26 to 0.91, and total allele numbers ranging from three to 16. The inbreeding coefficient values were generally higher in the Piauí population (ranging from − 0.593 to 0.762) than in the Mato Grosso population (ranging from − 1 to 0.575). The differences observed between those disjunct populations suggest they harbor different alleles, which has implications for Cerrado conservation strategies. Those loci will be useful for population studies of A. edulis.

Development and Utilization of Microsatellite Markers to Assess Genetic Variation Coupled with Modelling Range Shifts of Dodonaea Viscosa in Isolated Taita Hills and Mount Kenya Forests

“…Plants exhibit variation of SSRs Repeat units within their genomes, for example, in Paleosuchus trigonatus, di-nucleotide repeat motif predominated [50], whereas tetra-nucleotide repeats were the most frequent in Coffea canephora [51], and tri-nucleotide repeat motifs being most frequent in Hyalessa fuscata [52]. The mono-nucleotide repeat motifs predominated in our study, which is similar to Alibertia edulis [53]. Among the di-nucleotide repeats, AG/CT was the most abundant, followed by AT/AT and AC/GT while GC/CG repeats were extremely rare in all the Di-nucleotide repeats.…”

The genetic legacy of fragmentation and overexploitation in the threatened medicinal African pepper-bark tree, Warburgia salutaris

“…Although these markers are species specific, the increasing accessibility to next-generation sequencing 41 has enabled the development of SSRs for the so-called orphan, neglected or wild crop relative species (e.g. [42][43][44][45] ), although sequencing large plant genomes still remains a challenge 46 .…”