Development of Synthetic Gene Circuits to Amplify the Production of Biomaterials, Cellulases, and Lentivirus

Abstract

permutations for SRF and for SRF-MRTF complexes. The power of this approach allows us to investigate all possible consensus (64 possible sequences) and single mutant (768 possible sequences) using traditional EMSA-based approaches. Additionally we aim to identify cooperativity effects between MRTF and SRF in a sequence dependent fashion. These sequence sets (consensus and single mutants) account for up to 70% of all naturally occurring murine CArG boxes and understanding this energy landscape will facilitate the development of thermodynamic models for understanding the gene specific, mechanically driven behavior of MRTF/SRF across the genome. These models may be useful in understanding a variety of soft-tissue fibrotic conditions and other pathological processes, like metastasis, that appear MRTF/SRF responsive.