Development of surface plasmon resonance imaging biosensors for detection of ubiquitin carboxyl-terminal hydrolase L1

Sankiewicz, Anna; Laudański, Piotr; Romanowicz, Lech; Hermanowicz, Adam; Roszkowska-Jakimiec, W; Dębek, Wojciech; Gorodkiewicz, Ewa

doi:10.1016/j.ab.2014.09.021

Cited by 35 publications

(35 citation statements)

References 32 publications

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“…Activation of the antibody was performed by adding the mixture of NHS and EDC (1 : 1) in a carbonate buffer solution (pH 8Á5) into the antibody solution and with vigorous stirring for 5 min at the room temperature. Three ml of this solution was placed on the active places with the amine-modified surface, and incubated at for 1 h' [32].…”

Section: Chip Preparationmentioning

confidence: 99%

“…According to Sankiewicz et al, 'This is a label-free, surfacesensitive spectroscopic technique used to examine the interaction between biomolecules' [30][31][32]. SPRI is based on detection of changes in the refractive index within a short distance from the surface of a thin metal film caused by molecules bound to the metal surface [32]. The researched substance is captured from the solution by the antibody or inhibitor [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

“…SPRI is based on detection of changes in the refractive index within a short distance from the surface of a thin metal film caused by molecules bound to the metal surface [32]. The researched substance is captured from the solution by the antibody or inhibitor [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

“…lysosomal proteases, 20S proteasome, ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) and immunoproteasome [24,[32][33][34][35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

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Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Matuszczak

Sankiewicz

Dębek

et al. 2017

Clinical and Experimental Immunology

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SummaryThe aim of this study was to determinate the immunoproteasome concentration in the blood plasma of children with appendicitis, and its correlation with circulating proteasome and ubiquitin carboxyl-terminal hydrolase L1 (UCHL1). Twenty-seven children with acute appendicitis, managed at the Paediatric Surgery Department, were included randomly into the study (age 2 years 9 months up to 14 years, mean age 9Á5 6 1 years). There were 10 girls and 17 boys; 18 healthy, age-matched subjects, admitted for planned surgeries served as controls. Mean concentrations of immunoproteasome, 20S proteasome and UCHL1 in the blood plasma of children with appendicitis before surgery 24 h and 72 h after the appendectomy were higher than in the control group. The immunoproteasome, 20S proteasome and UCHL1 concentrations in the blood plasma of patients with acute appendicitis were highest before surgery. The immunoproteasome, 20S proteasome and UCHL1 concentration measured 24 and 72 h after the operation decreased slowly over time and still did not reach the normal range (P < 0Á05). There was no statistical difference between immunoproteasome, 20S proteasome and UCHL1 concentrations in children operated on laparoscopically and children after classic appendectomy. The immunoproteasome concentration may reflect the metabolic response to acute state inflammation, and the process of gradual ebbing of the inflammation may thus be helpful in the assessment of the efficacy of treatment. The method of operation -classic open appendectomy or laparoscopic appendectomy -does not influence the general trend in immunoproteasome concentration in children with appendicitis.

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Section: Chip Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…lysosomal proteases, 20S proteasome, ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) and immunoproteasome [24,[32][33][34][35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

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Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Matuszczak

Sankiewicz

Dębek

et al. 2017

Clinical and Experimental Immunology

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“…SPR sensing has been significantly developed in recent years to quantify biomarkers in complex matrixes, such as serum (Choi et al, 2010;Su et al, 2008 andLadd et al, 2009), plasma (Teramura et al, 2007), saliva (Yang et al, 2005) and cell lysate (Kyo et al, 2005). SPR biosensors have been shown to be suited for the detection of several cancer biomarkers (Sankiewicz et al, 2015) in solubilized samples, such as oropharyngeal squamous cell carcinoma (Liu et al, 2012), prostate cancer (Choi et al, 2010), colorectal, gastric, and pancreatic cancer (Su et al, 2008), intestinal cancer (Ladd et al, 2009), liver cancer (Teramura et al, 2007) and ovarian cancer (Liu et al, 2012) among others. However, serum and cell lysate screening on SPRi provide real time detection results in less time with low human error, but due to their protein and lipid rich matrix respectively, they expected to significantly foul on sensor surface.…”

Section: Introductionmentioning

confidence: 99%

Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

Singh

Nand

Sarita

et al. 2019

Arabian Journal of Chemistry

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Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), a-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDIÀTOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this

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SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological material

et al. 2016

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A new method for the selective determination of laminin-5 concentration using a biosensor and surface plasmon resonance imaging (SPRI) technique is presented. A biosensor based on the specific interaction of laminin-5 with rabbit polyclonal antibody was constructed. The analytically useful dynamic response range of the biosensor is between 0.014 and 0.1 ng mL−1. The detection limit is 4 pg mL−1. The potential influence of interferences on the SPRI signal was investigated, and the high selectivity of the biosensor was confirmed. In order to demonstrate the potential application of the biosensor, laminin-5 concentration in blood plasma was determined. The results were compared with the laminin-5 concentration obtained by the commercial enzyme-linked immunosorbent assay (ELISA) kit. A comparison of results from healthy donors obtained by SPRI measurement and ELISA indicates that they are close and shows good agreement with the data reported in the literature. The plasma samples of bladder cancer patients gave higher concentration measured with specific biosensor than by ELISA assay. The study shows the clear difference in concentration of laminin-5 in healthy humans and patients with bladder cancer. Extensive clinical studies using the newly developed method can result in an increase in the use of laminin-5 as a potential cancer marker.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-016-9621-x) contains supplementary material, which is available to authorized users.

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Development of surface plasmon resonance imaging biosensors for detection of ubiquitin carboxyl-terminal hydrolase L1

Cited by 35 publications

References 32 publications

Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological material

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