Development of Super-Resolution Microscopy—Application of Laguerre-Gaussian Beam to Microscopy—

Abstract

We developed super-resolution microscopy using the fluorescence depletion process. To verify the principle of this microscopy, we measured fluorescence images of micro-beads, i.e. the point-spread function (PSF). It was found that the FWHM of PSF becomes smaller than 110 nm. The results show that proposed microscopy can provide a sufficient spatial resolution to overcome the diffraction limit. In microscopy, the Laguerre-Gaussian beam with a zero center singularity plays an important role and determines performance of microscopy. We introduce our proposed technique, including formation about a Laguerre-Gaussian beam tightly focused by a high-NA microscope objective lens.

Fabrication of a Calibration Scale for Biological Fluorescence Microscopy Using Nano Imprinting

“…1) Since microscopy enables us to observe structures finer than 100 nm, the image properties in microscopy must be precisely calibrated. 2) A fine scale tool with a fluorescent structure of nanometer order is strongly required. 3) Thus, we developed a micro scale for fluorescence microscopy with an accuracy of 100 nm.…”