Development of Suberin Fatty Acids and Chloramphenicol-Loaded Antimicrobial Electrospun Nanofibrous Mats Intended for Wound Therapy

Tamm, Ingrid; Heinämäki, Jyrki; Laidmäe, Ivo; Rammo, Liisi; Paaver, Urve; Ingebrigtsen, Sveinung Gaarden; Škalko‐Basnet, Nataša; Halenius, Anna; Yliruusi, Jouko; Pitkänen, Pauliina; Alakurtti, Sami; Kogermann, Karin

doi:10.1016/j.xphs.2015.12.025

Cited by 24 publications

(15 citation statements)

References 49 publications

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“…PVA was used as emulsifier in fabrication of blended electrospun PLGA/chitosan nanofibers for potential skin reconstruction [113]. PVA and particularly PVP are important components of nanofibers delivering various biomolecules and drugs into skin, such as antibiotics (PVA [49], PVP [114]), kynurenine (PVA [48]), curcumin and HHC36 antimicrobial peptides (PVP [8]) or antimicrobial suberin fatty acids isolated from outer birch bark (PVP [115]). PVA and PVP were combined in electrospun nanofibrous membranes designed for sustained release of the antibiotic ciprofloxacin into wounds [116].…”

Section: Nanofibers From Synthetic Degradable Polymersmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bačáková¹,

Zikmundová²,

Pajorová³

et al. 2020

Applications of Nanobiotechnology

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Nanofibrous scaffolds are popular materials in all areas of tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. In this chapter, we focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is an organ with several vitally important functions, particularly barrier, thermoregulatory, and sensory functions. Nanofibrous meshes not only serve as carriers for skin cells but also can prevent the penetration of microbes into wounds and can keep appropriate moisture in the damaged skin. The nanofibrous meshes have been prepared from a wide range of synthetic and nature-derived polymers. This review is concentrated on synthetic non-degradable and degradable polymers, which have been explored for skin tissue engineering and wound healing. These synthetic polymers were often combined with natural polymers of the protein or polysaccharide nature, which improved their attractiveness for cell colonization. The nanofibrous scaffolds can also be loaded with various bioactive molecules, such as growth factors, hormones, vitamins, antioxidants, antimicrobial, and antitumor agents. In advanced tissue engineering approaches, the cells on the nanofibrous scaffolds are cultured in dynamic bioreactors enabling appropriate mechanical stimulation of cells and at air-liquid interface. This chapter summarizes recent results achieved in the field of nanofiber-based skin tissue engineering, including results of our research group.

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Section: Nanofibers From Synthetic Degradable Polymersmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bačáková¹,

Zikmundová²,

Pajorová³

et al. 2020

Applications of Nanobiotechnology

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“…= 10 mm) at ambient conditions. A method developed by Tamm et al [42] was used in a slightly modified format. Shortly, circular samples ( printer and printer System 30M (Hyrel 3D, Norcross, GA, USA).…”

Section: Stability Studymentioning

confidence: 99%

“…In the latter case, a good interaction between the dressing and the skin contributes to the absorption of excessive wound fluid and promotes healing by maintaining a moist environment [63]. Ideally, the wound dressings should be self-adhesive to the wound surface, yet easy and painless to remove [42]. Polymeric wound dressings are widely used; besides, if crosslinking is used to enhance their robustness, the adhesive properties of the formulations might be weakened [64,65], which was also apparent in the current study.…”

Section: Bioadhesion Within Simulated Wound Fluidmentioning

confidence: 99%

“…Polymeric wound dressings are widely used; besides, if crosslinking is used to enhance their robustness, the adhesive properties of the formulations might be weakened [64,65], which was also apparent in the current study. Furthermore, the incorporation of APIs and/or use of polymer blends in the electrospun NF mats might significantly affect their bioadhesion, as it has been demonstrated with polyvinylpyrrolidone [42]. Thus, a balance between the mechanical and adhesive properties should be aimed for.…”

Section: Bioadhesion Within Simulated Wound Fluidmentioning

confidence: 99%

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Bi-Layered Polymer Carriers with Surface Modification by Electrospinning for Potential Wound Care Applications

et al. 2019

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Polymeric wound dressings with advanced properties are highly preferred formulations to promote the tissue healing process in wound care. In this study, a combinational technique was investigated for the fabrication of bi-layered carriers from a blend of polyvinyl alcohol (PVA) and sodium alginate (SA). The bi-layered carriers were prepared by solvent casting in combination with two surface modification approaches: electrospinning or three-dimensional (3D) printing. The bi-layered carriers were characterized and evaluated in terms of physical, physicochemical, adhesive properties and for the safety and biological cell behavior. In addition, an initial inkjet printing trial for the incorporation of bioactive substances for drug delivery purposes was performed. The solvent cast (SC) film served as a robust base layer. The bi-layered carriers with electrospun nanofibers (NFs) as the surface layer showed improved physical durability and decreased adhesiveness compared to the SC film and bi-layered carriers with patterned 3D printed layer. Thus, these bi-layered carriers presented favorable properties for dermal use with minimal tissue damage. In addition, electrospun NFs on SC films (bi-layered SC/NF carrier) provided the best physical structure for the cell adhesion and proliferation as the highest cell viability was measured compared to the SC film and the carrier with patterned 3D printed layer (bi-layered SC/3D carrier). The surface properties of the bi-layered carriers with electrospun NFs showed great potential to be utilized in advanced technical approach with inkjet printing for the fabrication of bioactive wound dressings.Pharmaceutics 2019, 11, 678 2 of 21 ingredients and/or active pharmaceutical ingredients (APIs), such as antibiotics, anti-inflammatory agents, vitamins and growth factors, are continuously being developed [3,5].Wound dressings are produced by different methods depending on the desired structure and the materials used. In the current study, three commonly applied methods were exploited: solvent casting, extrusion-type three-dimensional (3D) printing and electrospinning. In solvent casting, polymeric films are prepared from drying of viscous solutions of polymer(s) with/without additives and active substances in a uniformly distributed layer [6,7]. Due to the simplicity of this method, solvent casting can be time-consuming, and the properties and stability of the films are dependent on the materials used [6,7]. Nevertheless, solvent cast (SC) polymer films are structurally more robust, and highly suitable to be exploited as base layer in multi-layered formulations.Electrospinning is a versatile technology for the fabrication of polymeric fibers with good diffusion characteristics and high surface area to volume ratio, which are beneficial for wound care applications to hinder bleeding, absorb excessive wound fluid and promote tissue regeneration [4,8,9]. The stability, reproducibility and production yield of the electrospinning process is highly dependent on the compatibility of the material...

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“…As with silver, most research here focuses on improving delivery systems to better deliver drug payload. 80 The use of a chitosan hydrogel system has become a common delivery method, owing to its favourable release properties and demonstrated inherent antimicrobial efficacy. 81 In combination with a bi-layered intelligent scaffold design, a chitosan containing nanoparticles demonstrates bivalent efficacy in the delivery of anti-bacterial and pro clotting factors [ Fig.…”

Section: Antibacterial Treatment Strategiesmentioning

confidence: 99%

Review: Multimodal bioactive material approaches for wound healing

2018

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Wound healing is a highly complex process of tissue repair that relies on the synergistic effect of a number of different cells, cytokines, enzymes, and growth factors. A deregulation in this process can lead to the formation of a non-healing chronic ulcer. Current treatment options, such as collagen wound dressings, are unable to meet the demand set by the wound environment. Therefore, a multifaceted bioactive dressing is needed to elicit a targeted affect. Wound healing strategies seek to develop a targeted effect through the delivery of a bioactive molecule to the wound by a hydrogel or a polymeric scaffold. This review examines current biomaterial and small molecule-based approaches that seek to develop a bioactive material for targeted wound therapy and accepted wound healing models for testing material efficacy.

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Development of Suberin Fatty Acids and Chloramphenicol-Loaded Antimicrobial Electrospun Nanofibrous Mats Intended for Wound Therapy

Cited by 24 publications

References 49 publications

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bi-Layered Polymer Carriers with Surface Modification by Electrospinning for Potential Wound Care Applications

Review: Multimodal bioactive material approaches for wound healing

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