Development of Stereo NIR-II Fluorescence Imaging System for 3D Tumor Vasculature in Small Animals

Su, Shih-Po; Lin, Syue-Liang; Chan, Yang-Hsiang; Lee, Yi Jang; Lee, Yun-Chen; Zeng, P.; Li, Yixuan; Yang, Muh‐Hwa; Chiang, Huihua Kenny

doi:10.3390/bios12020085

Cited by 3 publications

(6 citation statements)

References 48 publications

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“…The NIR-II rotational stereo imaging system has a better depth resolution and a larger view angle than the device developed in a previous study. 35 The performance of this system was quantified via the application of a This study reconstructed the depth maps of tumor vasculature and abdominal vessels of mice with the 360-deg rotational stereo method (Figs. 4 and 5), along with a rotational stage, as a substitute for a moving place, to improve the depth resolution with a larger stereo baseline.…”

Section: Discussionmentioning

confidence: 99%

“…1), boasting a 360-deg rotational stereo imaging technique, an NIR-II dye, and an NIR-II camera dedicated to small animal 3D NIR-II imaging. The NIR-II rotational stereo imaging system has a better depth resolution and a larger view angle than the device developed in a previous study 35 . The performance of this system was quantified via the application of a rotational stereo imaging technique to successfully reconstruct a phantom, whole-body vasculature, tumor blood vessels, and mice contour.…”

Section: Discussionmentioning

confidence: 99%

“…However, the NIR-I window is flawed with optical diffusion, lower resolution, and limitation of tissue penetration to 3 mm in depth, problems that can be rectified with the substitution of the NIR-II window, boasting increased tissue penetration depth, and enhanced imaging contrast 33 , 34 . Our previous study built a stereo NIR-II fluorescence imaging system with a planner moving plate for reconstructing 3D tumor vascular and blood vessel images with one NIR-II camera only 35 . The system overcomes the problem of substantial background noise due to much lower scattering in the NIR-II region, but it is flawed with a limited view angle and the need for a large operating platform.…”

Section: Introductionmentioning

confidence: 99%

“… 33 , 34 Our previous study built a stereo NIR-II fluorescence imaging system with a planner moving plate for reconstructing 3D tumor vascular and blood vessel images with one NIR-II camera only. 35 The system overcomes the problem of substantial background noise due to much lower scattering in the NIR-II region, but it is flawed with a limited view angle and the need for a large operating platform.…”

Section: Introductionmentioning

confidence: 99%

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Realization of NIR-II 3D whole-body contour and tumor blood vessels imaging in small animals using rotational stereo vision technique

Lee

Lin

et al. 2023

J. Biomed. Opt.

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Significance: Optical imaging in the second near-infrared (NIR-II, 1000 to 1700 nm) region is capable of deep tumor vascular imaging due to low light scattering and low autofluorescence. Non-invasive real-time NIR-II fluorescence imaging is instrumental in monitoring tumor status.Aim: Our aim is to develop an NIR-II fluorescence rotational stereo imaging system for 360-deg three-dimensional (3D) imaging of whole-body blood vessels, tumor vessels, and 3D contour of mice.Approach: Our study combined an NIR-II camera with a 360-deg rotational stereovision technique for tumor vascular imaging and 3D surface contour for mice. Moreover, self-made NIR-II fluorescent polymer dots were applied in high-contrast NIR-II vascular imaging, along with a 3D blood vessel enhancement algorithm for acquiring high-resolution 3D blood vessel images. The system was validated with a custom-made 3D printing phantom and in vivo experiments of 4T1 tumor-bearing mice. Results:The results showed that the NIR-II 3D 360-deg tumor blood vessels and mice contour could be reconstructed with 0.15 mm spatial resolution, 0.3 mm depth resolution, and 5 mm imaging depth in an ex vivo experiment. Conclusions:The pioneering development of an NIR-II 3D 360-deg rotational stereo imaging system was first applied in small animal tumor blood vessel imaging and 3D surface contour imaging, demonstrating its capability of reconstructing tumor blood vessels and mice contour. Therefore, the 3D imaging system can be instrumental in monitoring tumor therapy effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Realization of NIR-II 3D whole-body contour and tumor blood vessels imaging in small animals using rotational stereo vision technique

Lee

Lin

et al. 2023

J. Biomed. Opt.

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“…Fortunately, the NIR-II window can overcome the challenges of existing imaging techniques, offering increased tissue penetration depth and improved imaging contrast 8 . Our previous study developed a stereo NIR-II fluorescence imaging system to reconstruct 3D tumor vascular and blood vessel images with a single NIR-II camera 9 . This system benefits from the lower scattering in the NIR-II region but has a limited view angle and needs a large operating platform.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional NIR-II tumor blood vessels and bone imaging using rotational stereo vision in small animals

Su,

Chiang

2023

Diffuse Optical Spectroscopy and Imaging IX

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Optical imaging in the second near-infrared (NIR-II, 1000-1700 nm) is suitable for visualizing tumor vascular imaging and targeting bone structures in small animals. In this study, we developed a novel three-dimensional (3D) NIR-II fluorescence rotational stereo imaging system for conducting 3D tumor vascular imaging and bone imaging in mice with only one NIR-II camera and a rotational stereo vision technique. Our system utilizes a 3D blood vessel reconstruction algorithm to present high-resolution 3D blood vessel maps and skeletal systems through two camera views. This technique allows for the visualization of bone structure and the identification of metabolic diseases such as osteoporosis. We validated the system with custom-made 3D printing phantoms and 4T1 tumor-bearing mice, demonstrating the ability to accurately recover the tumor blood vessels and bones with an imaging depth of 5 mm, an image resolution of 0.15 mm, and a depth resolution of 0.35 mm. This pioneering development provides an effective tool for non-invasive real-time NIR-II fluorescence imaging and is instrumental in understanding the effects of cancer treatments on tumor vessels and bone structure in small animals.

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Short-wave infrared fluorescence imaging of near-infrared dyes with robust end-tail emission using a small-animal imaging device

Arena,

La Cava,

Faletto

et al. 2023

PNAS Nexus

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Commercially available NIR dyes, including Indocyanine Green (ICG), display an end-tail of the fluorescence emission spectrum detectable in the short-wave infrared (SWIR) window. Imaging methods based on the second near-infrared spectral region (1000-1700 nm) are gaining interest within the biomedical imaging community due to minimal autofluorescence and scattering, allowing higher spatial resolution and depth sensitivity. Using a SWIR fluorescence imaging device, the properties of ICG vs. heptamethine cyanine dyes with emission >800 nm were evaluated using tissue-simulating phantoms and animals experiments. In this study, we tested the hypothesis that an increased rigidity of the heptamethine chain may increase the SWIR imaging performance due to the bathochromic shift of the emission spectrum. Fluorescence SWIR imaging of capillary plastic tubes filled with dyes was followed by experiments on healthy animals in which a time series of fluorescence hindlimb images were analysed. Our findings suggests that higher spatial resolution can be achieved even at greater depths (> 5 mm) or longer wavelength (>1100 nm), both in tissue phantoms and in animals, opening the possibility to translate the SWIR prototype towards clinical application.

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Development of Stereo NIR-II Fluorescence Imaging System for 3D Tumor Vasculature in Small Animals

Cited by 3 publications

References 48 publications

Realization of NIR-II 3D whole-body contour and tumor blood vessels imaging in small animals using rotational stereo vision technique

Realization of NIR-II 3D whole-body contour and tumor blood vessels imaging in small animals using rotational stereo vision technique

Three-dimensional NIR-II tumor blood vessels and bone imaging using rotational stereo vision in small animals

Short-wave infrared fluorescence imaging of near-infrared dyes with robust end-tail emission using a small-animal imaging device

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