Development of spermatogenesis in captive-bred Spix's yellow-toothed cavy (Galea spixii)

Santos, Paulo R. S.; Oliveira, Moacir Franco de; Silva, Alexandre Rodrigues; Neto, Antônio Chaves de Assis

doi:10.1071/rd12015

Cited by 20 publications

(21 citation statements)

References 33 publications

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“…Researches related with reproductive biology have shown that G. spixii has continuous poliestral cycle, developing a type of inverted choriovitellinic placenta during pregnancy (Oliveira et al 2008; gestation period lasts about 48 days (Oliveira et al 2008); the onset of puberty in males occurs after 45 th postnatal day (Santos et al 2012); and females have masculinized external genitalia (Santos et al 2014b).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

et al. 2015

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RESUMO.-[Caracterização do ciclo estral de Galea spixii (Wagler, 1831).]Os Galea spixii habitam a vegetação semiá-rida da Caatinga, no Nordeste brasileiro. Eles são criados em cativeiro para realização de pesquisas relacionadas a biologia da reprodução. Sendo assim, o objetivo deste trabalho foi caracterizar o ciclo estral de G. spixii para obtenção de informações que melhorem o conhecimento do manejo reprodutivo da espécie em cativeiro. O ciclo estral foi monitorado por citologia esfoliativa vaginal em doze fêmeas adultas. Após a detecção de dois ciclos completos em cada animal, os mesmos foram eutanasiados. Em seguida foi realizado estudo histológico do epitélio vaginal com três fêmeas em cada fase do ciclo estral; cinco foram pareadas com machos para realização do grupo controle e outras três fêmeas foram utilizadas para monitorar a formação e ruptura da membrana de oclusão vaginal. Através de citologia esfoliativa vaginal, constatou-se predomínio de células superficiais em estro, células intermediárias grandes em proestro, células intermediárias pequenas e células parabasais com presença de neutrófilos em diestro e metaestro, respectivamente. O es- The Galea spixii inhabits semiarid vegetation of Caatinga in the Brazilian Northeast. They are bred in captivity for the development of researches on the biology of reproduction. Therefore, the aim of this study is characterize the estrous cycle of G. spixii, in order to provide information to a better knowledge of captive breeding of the species. The estrous cycle was monitored by vaginal exfoliative cytology in 12 adult females. After the detection of two complete cycles in each animal, the same were euthanized. Then, histological study of the vaginal epithelium, with three females in each phase of the estrous cycle was performed; five were paired with males for performing the control group for estrous cycle phases, and three other were used to monitor the formation and rupture of vaginal closure membrane. By vaginal exfoliative cytology, predominance of superficial cells in estrus, large intermediate cells in proestrus, intermediate and parabasal cells, with neutrophils, in diestrus and metestrus respectively was found. Estrus was detected by the presence of spermatozoa in the control group. By histology, greater proliferation of the vaginal epithelium in proestrus was observed. We conclude that the estrous cycle of G. spixii lasts 15.8 ± 1.4 days and that the vaginal closure membrane develops until complete occlusion of the vaginal ostium, breaking after few days. Future studies may reveal the importance of this fact for the reproductive success of this animal. INDEX TERMS: Galea spixii, cavies, female, preservation, reproduction, rodents. Characterization of the estrous cycle in

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Section: Introductionmentioning

confidence: 99%

Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

et al. 2015

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“…The literature on the Spix's yellow-toothed cavy is restricted to studies on vascularisation (Oliveira et al 2011;Original Paper Veterinarni Medicina, 61, 2016 (6): 337-343 doi: 10.17221/206/2015-VETMED Oliveira et al 2012a;Oliveira et al 2013), placentation (Oliveira et al 2012b;Vale et al 2013) and reproduction (Santos et al 2012), whereas studies on the nervous system are limited to the lumbosacral plexus (Oliveira et al 2010;Oliveira et al 2014). The study of the morphology of species found in the wild is assuming growing importance, especially of those species with zootechnical potential or biological value, as is the case with Galea spixii, a rodent of great importance, since their meat is used as a source of animal protein for poor people or even more broadly owing to cultural habits in the north-eastern part of Brazil.…”

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confidence: 99%

Origin and distribution of the brachial plexus in the Spix's yellow-toothed cavy (Galea spixii Wagler, 1831) (Rodentia, Caviidae)

Araujo¹,

Oliveira²,

Silva³

et al. 2016

Vet. Med. - Czech

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Due to the absence of literature on the brachial plexus in the Spix's yellow-toothed cavy, this study was conducted to examine the origin and neural distribution of this rodent's brachial plexus. Ten adult animals obtained from previous experiments were thawed, fixed in 10% formalin solution for 72 h and then dissected. Cotton blocks soaked in 2% hydrogen peroxide were placed on the ventral roots of the plexus. Photographs of the most representative specimens were taken and schematic sketches were prepared to illustrate the results. The Spix's yellow-toothed cavy's brachial plexus primarily originated from the ventral roots of the sixth (C 6 ), seventh (C 7 ) and eighth (C 8 ) cervical nerves and from the first two thoracic nerves (T 1 , T 2 ), and less frequently from C 6 -T 1 or C 6 -T 3 . The peripheral nerve trunks of the Spix's yellow-toothed cavy's brachial plexus are the following: suprascapularis (C 6 -C 7 ), axillaris (C 6 -C 7 ), subscapulares (C 6 -C 7 ), cranial pectoral (C 6 -C 7 ), musculocutaneous (C 7 -C 8 ), radialis (C 7 -T 2 ), medianus (C 7 -T 2 ), ulnaris (C 7 -T 2 ), pectorales caudales (C 7 -C 8 ), thoracicus longus (C 7 -C 8 ), thoracicus lateralis (C 7 -T 2 ) and thoracodorsalis (C 7 -T 2 ).Keywords: anatomy; brachial plexus; nervous system; rodent; Galea spixii List of abbreviations C 6 = ventral root of C 6 ; C 7 = ventral root of C 7 ; C 8 = ventral root of C 8 ; T 1 = ventral root of T 1 ; T 2 = ventral root of T 2 ; T 3 = ventral root of T 3

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“…It is known that sperm from different species could present a variation on its response to different hypoosmotic solutions, maybe due to a different membrane composition (Santos et al . ). In this sense, various studies have been conducted in order to determine the most appropriate osmolality for each different species, as a 60 mOsm/L solution for rabbits (Amorim et al .…”

Section: Discussionmentioning

confidence: 97%

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

et al. 2016

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da Silva, A.M., Bezerra, J.A.B., Campos, L.B., Praxedes, E.C.G., Lima, G.L. and Silva, A.R. 2017. Characterization of epididymal sperm from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831) recovered by different methods. -Acta Zoologica (Stockholm) 98: 285-291.The aim of the present study was to characterize the epididymal sperm of Spix's yellow-toothed cavy (Galea spixii) through two different recovery methods. Nine sexually mature males were euthanized and the complexes, testes-epididymis, were dissected. For each animal, one epididymis was processed by flotation method and the other was processed by retrograde flushing method, both using a TES-based buffered media. After recovery, we evaluated the sperm for motility, vigour, viability, functional membrane integrity and morphology. Morphometric data from the different sperm regions were evaluated by using an appropriate software. After recovery, both methods provide similar values for all the sperm parameters, aiming the recovery of more than 300 9 10 6 sperm, presenting >50% motile sperm, with normal morphology and functional membrane. The total sperm length in this sperm was 48.87 AE 0.1, and the sperm head presented 9.4%, on average. A notable characteristic was the prominent acrosome found in the G. spixii sperm. In conclusion, we demonstrate that either flotation or retrograde flushing methods are suitable for the recovery of sperm from cauda epididymis of Spix's yellow-toothed cavies.

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Development of spermatogenesis in captive-bred Spix's yellow-toothed cavy (Galea spixii)

Cited by 20 publications

References 33 publications

Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

Origin and distribution of the brachial plexus in the Spix's yellow-toothed cavy (Galea spixii Wagler, 1831) (Rodentia, Caviidae)

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

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