Development of specific markers for identification of Indian isolates of Fusarium oxysporum f.sp. ricini

Reddy, Jayarama; Raoof, M. A.; Ulaganathan, Kandasamy

doi:10.1007/s10658-012-0047-9

Cited by 13 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular markers have been widely used for the identification, taxonomy, evolution, as well as genetic diversity of bacteria, protozoa, fungi, plants, and animals (17)(18)(19)(20)(21)(22)(23)(24), such as restriction fragment length polymorphism (RFLP) (25), random amplified polymorphic DNA (RAPD) (26), amplified fragment length polymorphism (AFLP) assays (27), simple sequence repeats (SSR) (28), inter-simple sequence repeats (ISSR) (29,30), and single-nucleotide polymorphisms (SNP) (31). Several reports on genetic diversity of V. virens have been published in recent years.…”

mentioning

confidence: 99%

Genetic Diversity Analysis Reveals that Geographical Environment Plays a More Important Role than Rice Cultivar in Villosiclava virens Population Selection

Wang

Shu

Liu

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

mentioning

confidence: 99%

Genetic Diversity Analysis Reveals that Geographical Environment Plays a More Important Role than Rice Cultivar in Villosiclava virens Population Selection

Wang

Shu

Liu

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

“…Earlier, several workers have developed SCAR markers for the detection of Fusarium spp. in different crops like pigeon pea (Prasanthi et al 2015), castor (Reddy et al 2012), chickpea (Dubey et al 2014;Farahani et al 2015), tomato (Mutlu et al 2015) and banana (Cunha et al 2015). Whereas, Luongo et al (2012) developed a SCAR marker based on RAPD which was highly specific to detect the race 2 of F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic relationship among Indian population of Fusarium oxysporum f. sp. lentis infecting lentil and development of specific SCAR markers for detection

Hiremani

Dubey

2019

3 Biotech

View full text Add to dashboard Cite

The present study was taken up to understand the phylogenetic relationship using ITS and TEF markers among 22 isolates of Fusarium oxysporum f. sp. lentis (Fol) causing lentil wilt belonging to eight races isolated from different geographic locations of India and to develop specific markers for its detection. The nucleotide sequences of ITS region varied from 490 to 560 bp whereas, 670-725 bp for TEF 1α. The phylogeny analysis revealed that the isolates were more than 98% similar based on the neighbour joining analysis and were grouped into two major clusters in both ITS and TEF. The first major cluster of ITS had twenty isolates whereas for TEF, there were 15 isolates. Two sets of SCAR markers MS1 (162 bp) and MS2 (125 bp) were designed and synthesised. These markers were used against race representative Fol isolates for amplification. While, MS 1 marker was able to detect the genomic DNA up to 0.1 ng, MS 2 could detect the Fol genomic DNA up to 0.05 ng. The specificity of these two markers to detect Fol and their inability to amplify most common lentil pathogens (Rhizoctonia solani, R. bataticola, Sclerotium rolfsii, Sclerotinia sclerotiarum, and Aschochyta rabiei) makes them a reliable tool for detection. The phylogenetic analysis is helpful in the understanding of variability in Fol populations and the SCAR markers help in rapid and reliable detection of an important pathogen of lentil.

show abstract

Quick and accurate detection of Fusarium oxysporum f. sp. carthami in host tissue and soil using conventional and real-time PCR assay

Singh

Kapoor

2018

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Development of specific markers for identification of Indian isolates of Fusarium oxysporum f.sp. ricini

Cited by 13 publications

References 16 publications

Genetic Diversity Analysis Reveals that Geographical Environment Plays a More Important Role than Rice Cultivar in Villosiclava virens Population Selection

Genetic Diversity Analysis Reveals that Geographical Environment Plays a More Important Role than Rice Cultivar in Villosiclava virens Population Selection

Phylogenetic relationship among Indian population of Fusarium oxysporum f. sp. lentis infecting lentil and development of specific SCAR markers for detection

Quick and accurate detection of Fusarium oxysporum f. sp. carthami in host tissue and soil using conventional and real-time PCR assay

Contact Info

Product

Resources

About