Development of Specific Enzyme-Linked Immunosorbent Assays to Evaluate the Duck Immune Response After Experimental Infection with H5N1 and H7N1 Low Pathogenic Avian Influenza Viruses

Ferreira, Helena Lage; Pirlot, Jean François; Kaspers, Bernd; Kothlow, Sonja; Berg, Thierry van den; Lambrecht, Bénédicte

doi:10.1637/9202-875309-digest.1

Cited by 3 publications

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“… 2016 ; Ferreira et al. 2010 ). In this experiment, the serum IgG, serum IgA, intestine IgA, and liver IgA were isolated and analyzed by ELISA analysis.…”

Section: Resultsmentioning

confidence: 99%

Oral DNA vaccine adjuvanted with cyclic peptide nanotubes induced a virus-specific antibody response in ducklings against goose parvovirus

Wang

Chang

et al. 2023

Veterinary Quarterly

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Background Cyclic peptide nanotubes (cPNTs) formed from the spontaneous beta-sheet stacking of peptide rings may serve as a safe and effective oral delivery vehicle/adjuvant for DNA vaccines. Aim In this study, we sought to determine if a DNA vaccine expressing the VP2 protein of goose parvovirus, adjuvanted with cPNTs, may elicit virus-specific antibody response through oral vaccination. Material and methods Forty 20-day-old Muscovy ducks were randomly assigned to two groups of 20 ducks each and vaccinated. Ducks were orally vaccinated (Day 0) and boosted (Day 1 and Day 2) or were mock-vaccinated with saline as the negative control. For immunohistochemical staining, the primary antibody used comprised a rabbit anti-GPV antibody, and the secondary antibody was a goat anti-rabbit antibody. Goat-anti-mouse-IgG was used as a tertiary antibody. IgG and IgA antibody titers in serum were analyzed by the GPV virus-coated ELISA. For IgA antibody analysis, intestine lavage was harvested too. Results A DNA vaccine, coated with cPNTs, can induce a significant antibody response in ducklings. Immunohistochemical staining of tissues from vaccinated ducklings showed that VP2 proteins can be detected in the intestines and livers for up to six weeks, confirming the antigen expression by the DNA vaccine. Antibody analysis found that this vaccine formulation was very efficient at inducing IgA antibodies in the serum and the intestinal tract. Conclusion A DNA vaccine adjuvanted with cPNTs can effectively express the antigen and can significantly induce an antibody response against goose parvovirus through oral vaccination.

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“… 2016 ; Ferreira et al. 2010 ). In this experiment, the serum IgG, serum IgA, intestine IgA, and liver IgA were isolated and analyzed by ELISA analysis.…”

Section: Resultsmentioning

confidence: 99%

Oral DNA vaccine adjuvanted with cyclic peptide nanotubes induced a virus-specific antibody response in ducklings against goose parvovirus

Wang

Chang

et al. 2023

Veterinary Quarterly

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“…Parameter 2 -Mortality in wild species LPAI infections do not generally cause major clinical signs, nor do they cause mortality in wild birds (Ferreira et al, 2010;Bertran et al, 2012;Kuiken, 2013).…”

Section: Biodiversitymentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): low pathogenic avian influenza

Bøtner

Butterworth

et al. 2017

EFS2

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Low pathogenic avian influenza (LPAI) has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7 on disease profile and impacts, Article 5 on the eligibility of LPAI to be listed, Article 9 for the categorisation of LPAI according to disease prevention and control rules as in Annex IV and Article 8 on the list of animal species related to LPAI. The assessment has been performed following a methodology composed of information collection and compilation, expert judgement on each criterion at individual and, if no consensus was reached before, also at collective levels. The output is composed of the categorical answer, and for the questions where no consensus was reached, the different supporting views are reported. Details on the methodology used for this assessment are explained in a separate opinion. According to the assessment performed, LPAI can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL. The disease would comply with the criteria as in Sections 3 and 5 of Annex IV of the AHL, for the application of the disease prevention and control rules referred to in points (c) and (e) of Article 9(1). The animal species to be listed for LPAI according to Article 8(3) criteria are all species of domestic poultry and wild species of mainly Anseriformes and Charadriiformes, as indicated in the present opinion.

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“…It is known that influenza virus reproduces and accumulates in epithelium of the respiratory tract and intestine (portal of the infection). At the same time, according to literature data, the antibodies produced on surface of mucous membrane, may play an important role not only in protection, but also in restriction of primary replication on virus gateway and elimination portals [4], which increases an importance of mucosal immunity against influenza [5,6].…”

mentioning

confidence: 96%

“…The production of active oxygen forms, which leads to initiation and development of lipids' peroxidation in microorganism's biological membranes, is one of pathogenetic elements of influenza. Besides, immunoactive substances -immunoglobulins, and various classes of cytokines, are produced in epithelial cells [4,9]. At the same time, internal lesions and unspecific resistance system's functional status in mucosal membranes of poultry infected with avian influenza, are insufficiently studied.…”

mentioning

confidence: 99%

Dynamics of markers of non-specific resistance in trachea of chickens at orthomyxovirus infection

Коваленко¹,

Stegnij²

2018

Visnyk agrarnoi nauky

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The purpose. To fix dynamics of factors of non-specific immunoreactivity in mucous membrane of trachea of chickens experimentally infected with low-pathogenic strain of virus of grippe, as a model of pathogenesis of orthomyxovirus infection caused by virus of type A. Methods. Biochemical for research of homogenates of mucous membrane of trachea of chickens; statistical. Researches were carried out in 2 groups (n=35) of 45day's chickens. Birds of the first group were infected with low-pathogenic virus of bird's grippe (strain А/mallard/Ukraine/2007 (H5N2), the second group was a control one. Samples of trachea from 5 chickens from each group were taken on the 1, 3, 5, 7, 10, 14 and 21 day after infection. Markers of non-specific resistance in homogenates of mucous membrane of trachea were determined using methods adapted by us for operation with homogenates of mucous membrane. Results. Dynamics is studied of indexes describing the level of inborn mucosal immunity in trachea of a bird at experimental grippe within 21 day. It is determined that infect of chickens with low-pathogenic strain of virus of grippe raises to maximum the level of IgM-up to 48,3% on the third day. On the third day they also observed lowering of production of IgG (on 31,8%), in the period from 7th to 10th day density of IgG increased on 35,5%, and active accumulation of IgA began on the 10th day after infection. In dynamics of experiment they fixed heightening intensity of processes of lipoperoxidation (maximally on the first and the 21st day of experiment), oscillations of activity of catalase (on the 5th day of experiment the increase has made 88%, on the 21st day it lowed on 38,2%, Р<=0,05) and lowering of general anti-oxidant activity of lipids (for 21 day on 70,2%). Conclusions. The fixed changes in system of non-specific immunity of mucous membrane of trachea at experimental low-pathogenic grippe of birds within 21 day of evolution of infection can be used as a basis for development of strategy of struggle with orthomyxovirus infections and bird's grippe in particular, using special prophylactic means and immunomodulators of direct action.

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Development of Specific Enzyme-Linked Immunosorbent Assays to Evaluate the Duck Immune Response After Experimental Infection with H5N1 and H7N1 Low Pathogenic Avian Influenza Viruses

Cited by 3 publications

References 0 publications

Oral DNA vaccine adjuvanted with cyclic peptide nanotubes induced a virus-specific antibody response in ducklings against goose parvovirus

Oral DNA vaccine adjuvanted with cyclic peptide nanotubes induced a virus-specific antibody response in ducklings against goose parvovirus

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): low pathogenic avian influenza

Dynamics of markers of non-specific resistance in trachea of chickens at orthomyxovirus infection

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