Development of SNP markers for Ayous (Triplochiton scleroxylon K. Schum) an economically important tree species from tropical West and Central Africa

Transcriptome Analysis of Cinnamomum chago: A Revelation of Candidate Genes for Abiotic Stress Response and Terpenoid and Fatty Acid Biosyntheses

“…Transcriptome sequencing on multiple individuals is an effective way to identify and validate SNPs and SSRs ( Huang et al, 2015 ). The identification and validation of gene-associated SSRs and SNPs will provide an important tool in understanding the genetic basis of adaptive trait, population genetic structuring, and species relatedness for C. chago and other related species in Lauraceae ( Chen et al, 2015 ; Huang et al, 2015 ; Jardine et al, 2016 ).…”

Development of nuclear and plastid SNP markers for genetic studies of Dipteryx tree species in Amazonia

“…However, the amplification and scoring of microsatellite loci from timber material is problematic due to the low quality and quantity of DNA which can be recovered. New sets of single nucleotide polymorphisms (SNP) have been recently developed for valuable tropical timber species and have been successfully amplified in DNA of timber in South America (Chaves et al 2018;Meyer-Sand et al 2018) and Africa (Blanc-Jolivet et al 2017;Jardine et al 2016;Pakull et al 2016). Here we present a new set of 315 nuclear and plastid SNPs tested on Dipteryx species from which we selected 100 loci to be used for the genetic characterization of species and population genetic analyses.…”

Development of nuclear SNP markers for the timber tracking of the African tree species Sapelli, Entandrophragma cylindricum

“…DNA was extracted from dried cambium of leaves following (Dumolin et al 1995) and sent to Floragenex (Portland, Oregon, USA) for sequencing and SNP detection. RADseq procedure and SNP detection protocols were the same as described in Jardine et al 2016). We screened a total of 178 samples, recorded as E. cylindricum by our field teams, and distributed over the species distribution range, at the newly developed SNP loci (Table 1).…”

“…Until now, only nuclear microsatellites (nSSRs) have been developed to study the mating system and gene flow (Garcia et al 2004;Lourmas et al 2007) and have been used to control the forest concession of origin (Jolivet and Degen 2012). New sets of nSNPs have been recently developed for other valuable tropical timber species for traceability purposes Jardine et al 2016;Pakull et al 2016) and present several advantages over nSSRs (Blanc-Jolivet and Liesebach 2015). Here we present a new set of nSNPs for E. cylindricum optimized for low-cost tracking of geographical origin.…”