Development of Smartphone-Based Lateral Flow Device for the Quantification of LH and E3G Hormones

Thakur, Ritambhara; Akram, Farooq; Rastogi, Vivek; Mitra, Amit; Nawani, Rahul; Av, Varun; Dubey, Swapnil; Shakher, Chandra

doi:10.1109/jsen.2020.3008566

Cited by 11 publications

(10 citation statements)

References 20 publications

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“…While on the one hand the use of readers increases the cost per analysis, on the other it allows data digitalization, tracking, storage, and transmission reducing interpretation and transcription errors, thus ensuring testing quality and control [ 18 ]. In this sense, we are also witnessing an increasing exploitation of the built-in smartphone technology to be used as LFIA reader, as increasingly reported in the literature [ 122 , 132 , 267 , 268 , 269 , 270 , 271 , 272 , 273 , 274 , 275 ]. Moreover, the use of strip readers paved the way for the use of alternative detection methods (fluorescence, chemiluminescence, etc.)…”

Section: Evergreen and New Challengesmentioning

confidence: 99%

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Nardo

Chiarello

Cavalera

et al. 2021

Sensors

236

162

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The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clinical diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of molecules, organisms, and (bio)markers for clinical purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.

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Section: Evergreen and New Challengesmentioning

confidence: 99%

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Nardo

Chiarello

Cavalera

et al. 2021

Sensors

236

162

View full text Add to dashboard Cite

show abstract

“…This has allowed for improved sensitivity. In the laboratory, many LFAs have been linked to smartphone-readout systems; 72,73,86,88,89 however, these are not yet used routinely by patients. The future move toward read-out systems and the use of digital devices will enable tracking of patient health at home by clinicians, as the systems can be linked to existing healthcare systems in the hospital.…”

Section: Discussionmentioning

confidence: 99%

“…Whilst not as effective as medical contraception, hormone monitoring is simple and an alternative for women who do not wish to use other forms of contraception. Recently, an “Inito” device was developed 88 which, like the LFA by Bonnar et al , 87 measured both LH and E3G simultaneously. The test strips utilised blue latex particles.…”

Section: Research Advances In Hormone Lfasmentioning

confidence: 99%

Lateral flow assays for hormone detection

Khelifa

Jiang

et al. 2022

Lab Chip

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“…IFM captures the image of the test strip using the Inito mobile application and processes the image to yield the optical density (OD) which corresponds to the concentration of the metabolite. IFM uses a multi-scale algorithm to detect the device and eliminate the variations in resolution and aspect ratio due to smartphone variability 11 . Here we show that IFM is an effective urinary hormone monitor and has a laboratory-based ELISA-equivalent output that makes it ideal for predicting fertile days and confirming ovulation at home.…”

Section: Introductionmentioning

confidence: 99%

“…IFM captures the image of the test strip using the Inito mobile application and processes the image to yield the optical density (OD), which corresponds to the concentration of the metabolite. IFM uses a multiscale algorithm to detect the device and eliminate the variations in resolution and aspect ratio due to smartphone variability 11 .…”

Section: Introductionmentioning

confidence: 99%

A quantitative home-use framework for assessing fertility and identifying novel hormone trends by recording urine hormones

Pattnaik¹,

Das²,

Va³

2021

Preprint

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Fertility testing using urinary hormones has been used to improve the likelihood of pregnancy effectively. To provide fertility scores, the existing home-use systems measure one or two hormones. However, the hormone profiles vary depending on cycle duration, fertility-related disorders, drugs and other treatments. Here we introduce Inito, a mobile-phone connected reader that is scalable to multiple hormone tests. In this report, we assess the accuracy of the quantitative home-based fertility monitor, Inito Fertility Monitor (IFM), and suggest using IFM as a device to monitor and analyse female hormone patterns. We further show that Inito can be used as a tool to decipher novel hormone patterns in a more profound way than the existing knowledge in the field. There were two aspects to our analysis: i. Evaluation of Inito Fertility Monitor efficiency characteristics for urinary Estrone-3-glucuronide (E3G), Pregnanediol glucuronide (PdG) and Luteinizing hormone (LH) assessment, and ii. A retrospective study of patients' hormone profiles using IFM. For the performance assessment, the recovery percentage of the three hormones from IFM was evaluated using standard spiked solutions, the reproducibility of re-prediction and the correlation between re-predicted values from IFM and ELISA. The first urine sample of the day was collected from 100 women who volunteered for the study and IFM was provided to 52 more women to use it at home to track their fertile days. We observed that with all three hormones, IFM had an accurate recovery percentage and had a CV of less than 10 percent for separate test strips through tests of the same dosage making it a reliable tool to monitor hormone patterns of patients. Furthermore, in predicting the concentration of E3G, PdG and LH in urine samples, we show that IFM has a high correlation with ELISA. We show that certain hormone trends associated with urinary E3G, PdG and LH could be accurately captured using IFM. In addition, we report a novel criterion for earlier confirmation of ovulation compared to existing criteria. Finally, we present a novel hormone pattern associated with most of the menstrual cycles by examining hormone profiles from the volunteers recruited for the clinical trial. The Inito Fertility Monitor is an effective tool for calculating the urinary concentrations of E3G, PdG and LH and can also be used to provide accurate fertility scores and confirm ovulation. In addition, the sensitivity of IFM facilitates the monitoring of menstrual cycle-related hormone patterns, therefore also making it a great tool for physicians to track the hormones of their patients.

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Development of Smartphone-Based Lateral Flow Device for the Quantification of LH and E3G Hormones

Cited by 11 publications

References 20 publications

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Lateral flow assays for hormone detection

A quantitative home-use framework for assessing fertility and identifying novel hormone trends by recording urine hormones

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