Development of Small-Molecule Fluorescent Probes Targeting Enzymes

Abstract

As biological catalysts, enzymes are vital in controlling numerous metabolic reactions. The regulation of enzymes in living cells and the amount present are indicators of the metabolic status of cell, whether in normal condition or disease. The small-molecule fluorescent probes are of interest because of their high sensitivity and selectivity, as well as their potential for automated detection. Fluorescent probes have been useful in targeting particular enzymes of interest such as proteases and caspases. However, it is difficult to develop an ideal fluorescent probe for versatile purposes. In the future, the design and synthesis of enzyme-targeting fluorescent probes will focus more on improving the selectivity, sensitivity, penetration ability and to couple the fluorescent probes with other available imaging molecules/technologies.

Anti-Inflammatory Activity of Peptides from Ruditapes philippinarum in Lipopolysaccharide-Induced RAW264.7 Cells and Mice

“…Previous studies have confirmed that proteins can effectively release peptides after enzymatic hydrolysis via biological proteases [ 40 ]. However, the peptides released by different enzymatic methods differ in content and quantity due to the specificity of proteases, followed by the yield of short peptides, which is an important indicator reflecting the effect of enzymatic hydrolysis and is closely related to the extent of hydrolysis [ 41 ]. It can be seen in Figure 5 that PeCTHC had a higher short peptide yield of 28.01 ± 1.45%.…”

A comparative study of two thienopyrimidine Schiff base probes for sequential monitoring of Ga3+ and Pd2+

“…Grad characteristic and can discriminate Ga 3+ and Pa 2+ in H 2 O/DMF (1/9, v/v) solution [33]. Compared with other analytical skills, the technology of fluorescent sensing has garnered significant attention due to its high sensitivity, fast response, excellent selectivity, and low biological toxicity [34][35][36][37][38][39][40][41]. However, practical applications still face inconveniences such as complex preparation, cumbersome separation, and challenging purification processes [42][43][44][45].…”

Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System

“…Through this initiative, we have made substantial contributions to the field, having developed over 30 bioprobes ( Figure 1 ) [ 6 ]. Conventionally, fluorescent probe development focuses on binding to proteins, a method we termed Protein-Oriented Live-cell Distinction (POLD) [ 7 , 8 , 9 , 10 ]. Throughout the procedure, the protein-binding system encounters limitations in explaining the journey of fluorescent molecules, particularly in the absence of protein partners.…”