Development of Small-Diameter Vascular Grafts Through Decellularization of Human Blood Vessels

Porzionato, Andrea; Sfriso, Maria Martina; Pontini, Alex; Macchi, Veronica; Buompensiere, Maria Ida; Petrelli, Lucía; Bassetto, Franco; Vindigni, Vincenzo; De, Raffaele

doi:10.1166/jbt.2017.1545

Cited by 13 publications

(18 citation statements)

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“…Another application of decellularized ECM is improvement in the biological properties of synthetic materials [ 11 ] or fabrication of hydrogels [ 12 – 15 ]. Decellularization techniques have been successfully used for a variety of organs including the heart [ 16 ], blood vessels [ 17 ], urethra [ 18 ], urinary bladder [ 19 ], kidney [ 20 , 21 ], trachea [ 22 ], lung [ 23 ], skeletal muscle [ 24 ], liver [ 25 ], and gastrointestinal tract [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries

et al. 2018

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BackgroundThe increasing number of patients with ovarian insufficiency due to autoimmune disorders, genetic predisposition, or iatrogenic effects of treatment such as cancer therapies necessitates an urgent measure to find a safe and transplantable alternative ovary. A bioengineered ovary is one of the strategies on which the researchers have recently been working. An engineered ovary should be able to mimic the natural ovary aspects. Recent studies suggest that the decellularized organ-specific extracellular matrix-based scaffolds can serve as a native niche to bioengineering artificial organs. Therefore, we established a human decellularized ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated process, as an optimized protocol.MethodsThe human ovary samples were decellularized with 1% SLES for 48 h followed by DNase I in PBS for 24 h, and then thoroughly rinsed in PBS to remove the cell remnants and chemical reagents. Efficient cell removal was confirmed by DNA content analysis, hematoxylin and eosin, and Hoechst staining. Preservation assessment of the extracellular matrix structures was performed by immunohistochemistry, histological staining, and scanning electron microscopy. An MTT test was done to assess the in vitro scaffold’s cytocompatibility, and finally in vivo studies were performed to evaluate the biocompatibility, bioactivity, and secretion functions of the ovarian grafts made of primary ovarian cells (POCs) on the decellularized scaffolds.ResultsEvidence provided by SEM, histochemical, and immunohistochemical analyses showed that the ovarian extracellular matrix was preserved after decellularization. Moreover, MTT test indicated the suitable cytocompatibility of the scaffolds. The in vivo assessment showed that the POCs kept their viability and bioactivity, and reconstructed the primordial or primary follicle-like structures within the scaffolds after transplantation. Immunostaining characterized somatic cells that were capable of expressing steroid hormone receptors; also, as a marker of granulosa cell, inhibin-α immunostaining demonstrated these cells within the grafts. Additionally, hormone assessment showed that serum estradiol and progesterone levels were significantly higher in ovariectomized rats with ovarian cells-seeded grafts than those with or without decellularized scaffold grafts.ConclusionsA human ovary-specific scaffold based on a SLES-decellularized protocol as a biomimicry of the natural ovarian niche can be an ideal scaffold used to reconstruct the ovary.Electronic supplementary materialThe online version of this article (10.1186/s13287-018-0971-5) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries

et al. 2018

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“…Additionally, studies assessing the neo‐endothelialization of the HUV upon decellularization indicated mixed results, with some groups indicating a low cell adhesion and viability on the lumenal surface thus an inefficient endothelialization (Mangold et al, 2015). Low endothelialization has also been observed on other scaffold types, with EC not able to endure arterial flow conditions (Lin et al, 2018; Porzionato et al, 2017). Groups have thus focused on modifying the lumenal surface for increased EC adhesion.…”

Section: Discussionmentioning

confidence: 96%

“…EC were then seeded into the lumen and the construct was cultured for 10 more days leading to (Mangold et al, 2015). Low endothelialization has also been observed on other scaffold types, with EC not able to endure arterial flow conditions (Lin et al, 2018;Porzionato et al, 2017). Groups have thus focused on modifying the lumenal surface for increased EC adhesion.…”

Section: Discussionmentioning

confidence: 99%

“…One drawback, however, has been their difficult recellularization with, in the case of vascular development, the restricted migration of the SMC within the vascular wall due to the subcellular pore geometries that require active digestion/remodeling processes. Indeed, when implanted as cell‐free decellularized grafts, the infiltration of host cells (for instance, SMC) is typically limited to the anastomotic sites; moreover, re‐endothelialization is partial, particularly when under arterial flow (Lin, Hsia, Ma, Lee, & Lu, 2018; Porzionato et al, 2017). This was a main issue as a vascular medial layer replete with physiologically dense SMC is critical in maintaining long‐term mechanical graft integrity and conferring hemodynamic adaptability.…”

Section: Introductionmentioning

confidence: 99%

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Flow with variable pulse frequencies accelerates vascular recellularization and remodeling of a human bioscaffold

Walle

McFetridge

2020

J Biomedical Materials Res

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Despite significant advances in vascular tissue engineering, the ideal graft has not yet been developed and autologous vessels remain the gold standard substitutes for small diameter bypass procedures. Here, we explore the use of a flow field with variable pulse frequencies over the regeneration of an ex vivo-derived human scaffold as vascular graft. Briefly, human umbilical veins were decellularized and used as scaffold for cellular repopulation with human smooth muscle cells (SMC) and endothelial cells (EC). Over graft development, the variable flow, which mimics the real-time cardiac output of an individual performing daily activities (e.g., resting vs. exercising), was implemented and compared to the commonly used constant pulse frequency. Results

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“…Moreover, these tissue scaffolds should be able to resist physiological blood pressure, as they possess similar structure and mechanical properties as native vessels (Simsa et al, 2018, Lin et al, 2018. Studies showed good endothelialization and smooth muscle cell migration in the media for venous grafts, and partial recellularization of the media and adventitia for arterial grafts (Porzionato et al, 2017).…”

Section: Decellularized Matricesmentioning

confidence: 99%

Tissue engineered vascular grafts

2018

Biomater Tissue Eng Bull

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With the continuous increase in the prevalence of cardiovascular diseases and the limited efficiency of conventional treatments, including diet and lifestyle modifications, pharmaceutical administration, and surgical interventions, there is an equally increased need for vascular grafts for the replacement of damaged blood vessels. Currently, autologous grafts represent the gold standard. However, due to the limited availability, intensive work has been performed in the field of vascular tissue engineering. Although there are many possibilities for obtaining synthetic vascular grafts, there is still no ideal solution for vascular replacements. In this paper, the main material categories and fabrication techniques are reviewed.

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Development of Small-Diameter Vascular Grafts Through Decellularization of Human Blood Vessels

Cited by 13 publications

References 0 publications

Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries

Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries

Flow with variable pulse frequencies accelerates vascular recellularization and remodeling of a human bioscaffold

Tissue engineered vascular grafts

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