2021
DOI: 10.1016/j.jviromet.2021.114145
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Development of singleplex and multiplex real-time (Taqman®) RT-PCR assays for the detection of viruses associated with fig mosaic disease

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Cited by 8 publications
(4 citation statements)
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“…[ 15 ], who found FMV, FLMaV-1, FLMaV-2, and FMMaV in fig leaf samples, and Ref. [ 30 ], who found FMV, FLMaV-1, FLMaV-2, FMMaV, FFKaV, FCV-1, and FLV-1 in fig leaf samples during a preliminary survey conducted on a small number in some Egyptian governorates. In comparison with our findings, all 137 tested trees from all cultivars were infected by at least one virus, with FMV and FLMaV-2 being the most prevalent viruses with incidence rates of 49% and 21.8% in all tested plants, respectively, followed by FLMaV-1 with an incidence rate of 10.9% in all tested plants.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…[ 15 ], who found FMV, FLMaV-1, FLMaV-2, and FMMaV in fig leaf samples, and Ref. [ 30 ], who found FMV, FLMaV-1, FLMaV-2, FMMaV, FFKaV, FCV-1, and FLV-1 in fig leaf samples during a preliminary survey conducted on a small number in some Egyptian governorates. In comparison with our findings, all 137 tested trees from all cultivars were infected by at least one virus, with FMV and FLMaV-2 being the most prevalent viruses with incidence rates of 49% and 21.8% in all tested plants, respectively, followed by FLMaV-1 with an incidence rate of 10.9% in all tested plants.…”
Section: Discussionmentioning
confidence: 99%
“…Various molecular approaches are currently highly effective in detecting viruses or viral particles in plants [ 23 , 28 , 29 ]. RT-PCR was therefore an accurate, rapid, and dependable technology used by several authors to detect FMD viruses [ 2 , 10 , 13 , 15 , 16 , 19 , 30 ]. In recent years, the use of nucleotide fragment sequence analysis and comparison with different fig virus isolates from around the world has significantly aided in the detection of fig mosaic disease viruses and understanding the ecology of the disease risk, emergence, and dynamics of fig mosaic disease [ 10 , 19 ].…”
Section: Introductionmentioning
confidence: 99%
“…In real-time PCR, controls must be prepared from the RM for each gene doping test [ 25 ], either by diluting high-concentration RMs for each test or by preparing diluted controls for long-term storage. The former method is generally used for quantitative operations requiring strict dilutions because a high concentration is more stable than a low concentration [ 1 ]. The latter method is easier to perform, but the stability of PTCs with low copy numbers is a concern.…”
Section: Discussionmentioning
confidence: 99%
“…Multiplex TaqMan real-time PCR has been applied widely for pathogen detection in humans [14,[24][25][26][27] and animals [10,[28][29][30][31][32][33][34][35]. Recently, this method has also been used to detect viruses in sweet potatoes, pome fruit trees, stone fruit trees, and fig trees [36][37][38][39][40]. However, this method has not been used for the simultaneous detection of LSV, LMoV, CMV, SYSV, and PlAMV in lily plants.…”
Section: Introductionmentioning
confidence: 99%