Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Abstract

The goal of this research is to develop a single-walled carbon nanotube-(SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference (Δ ) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor ( < 0.05). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log CFU/mL.

The Advances in Biomedical Applications of Carbon Nanotubes

“…Aside from DNA-detection, CNT-based sensing systems have been also actively studied towards other possible biomedical applications, including but not limited to, detection of glucose [56], pesticides [57], cholesterol [58], reactive oxygen species [59], lactate [60], cancer [61], tuberculosis [55], nucleic acids [62], malaria [63], Staphylococcus aureus [64], and anticancer drugs [65].…”

Recent Advances in Nanomaterial-Based Aptasensors in Medical Diagnosis and Therapy

“…CNT can be visualized as a graphite layer rolled into cylinder of a few nanometers diameter. Compared to other nanomaterials, CNTs possess a unique combi-nation of optical (near infrared luminescence), electrical (high mobility, high conductivity, higher electron transfer kinetics) and chemical (extremely high surface area 1300 m 2 /g, ability to be functionalized) properties which make CNTs suitable for various biomedical applications [90,91], A recent study investigated the binding specificity of Staphylococcus aureus by CNTs hybridized with 1-pyrenebutanoic acid succinimidyl ester (PBASE) where a moderate LOD of 4 log CFU/mL was achieved [92]. Immobilization of biomolecules on the surface of CNTs sometimes requires chemical modification, which may interfere with the electrical properties of CNTs [92,93].…”

A Review of Carbon Nanotubes Field Effect-Based Biosensors

“…In their SWCNT-biosensor developed for the detection of Staphylococcus aureus, Choi et al (2017) found that the optimum concentration of assembled SWCNTs was 0.1mg/mL to reduce the contact resistance with the gold electrodes fabricated on a silicon wafer [88]. Yet the resistance reported was in the excess of kilo-ohms.…”

“…High resistance has the effect of limiting the on-current, and hence resulting in reduced device performance in terms of limit of detection and sensitivity as aforementioned. This contact resistance may range from kiloohms to the excess of mega-ohms [14], [31], [73], [86], [88].…”