Development of Simultaneous Derivative Spectrophotometric and HPLC Methods for Determination of 17-Beta-Estradiol and Drospirenone in Combined Dosage Form

Abstract

Simple, rapid spectrophotometric, and reverse-phase high performance liquid chromatographic methods were developed for the concurrent analysis of 17-beta-estradiol (ESR) and drospirenone (DRS). The spectrophotometric method was based on the determination of first derivative spectra and determined ESR and DRS using the zero-crossing technique at 208 and 282 nm, respectively, in methanol. The linear range was 0.5–32.0 µg·mL−1 for DRS and 0.5–8.0 µg·mL−1 for EST. The limit of detection (LOD) values were 0.14 µg·mL−1 and 0.10 µg·mL−1 and limit of quantification (LOQ) values were 0.42 µg·mL−1 and 0.29 µg·mL−1 for ESR and DRS, respectively. The chromatographic method was based on the separation of both analytes on a C18 column with a mobile phase containing acetonitrile and water (70 : 30, v/v). Detection was performed with a UV-photodiode array detector at 279 nm. The linear range was 0.08–2.5 µg·mL−1 for DRS and 0.23–7.5 µg·mL−1 for EST. LOD values were 0.05 µg·mL−1 and 0.02 µg·mL−1 and LOQ values were 0.15 µg·mL−1 and 0.05 µg·mL−1 for ESR and DRS, respectively. These recommended methods have been applied for the simultaneous determination of ESR and DRS in their tablets.

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

“…A comparison has been done between some of analytical variables obtained from the proposed methods for determination of estradiol and progesterone with other previous published methods [11][12][13] and the results summarized in…”

“…In recent years, analytical methods ultraviolet spectrophotometric method 6,11 , derivative spectrophotometry DS [12][13][14] , HPLC 12,15 , IGC/MS stands for isotope dilution-gas chromatography/mass spectrometry 16 , LCTMS 17 , and GC-MS/MS 4,18 for estradiol and progesterone levels in human serum or plasma samples, as well as a pharmaceutical formulation, have been created. This study aims to develop new and very simple techniques for resolving overlap spectra in their mixtures and pharmaceutical formulations without the need for separation.…”