Development of simple and efficient in Planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens

Supartana, Putu; Shimizu, Tsutomu; Nogawa, Masahiro; Shioiri, Hidenari; Nakajima, Tadashi; Haramoto, Naoyuki; Nozue, Masayuki; Kojima, Mineo

doi:10.1263/jbb.102.162

Cited by 85 publications

(46 citation statements)

References 23 publications

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“…Transformation of these species is usually based on tissue culture technique that is time and money consuming (Supartana et al 2006). Establishing new method will increase the opportunity to transform these species.…”

Section: Resultsmentioning

confidence: 99%

“…Establishing new method will increase the opportunity to transform these species. In the present study, soaked seeds of wheat and barley were inoculated with small needle to introduce Agrobacterium harbouring pBI121CB or pBI121NB to shoot apices from which many organs and primordia will emerge (Supartana et al 2006). Co-cultivation was done for 5 days to increase the chance and efficiency of transformation (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Wheat and barley were transformed according to Supartana et al 2006. Each seed was pierced with small needle to facilitate the introduction of the Agrobacterium cells to the shoot apex.…”

Section: Wheat and Barley Co-cultivationmentioning

confidence: 99%

“…Agrobacterium-mediated transformation of these species mostly depends on tissue culture technique that is time and money consuming. Moreover, somatic mutation or somaclonal variation may occur during in vitro culture and some plants obstinate to regeneration (Supartana et al 2006). Additionally, transformation of wheat is restricted to narrow range of genotypes (Jones 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, transformation of wheat is restricted to narrow range of genotypes (Jones 2005). Supartana et al (2006) have reported new method for transforming Triticum aestivum L. (Var Shiranekomugi) by inoculating Agrobacterium into apical meristem of soaked seeds, from which various organs and leaf primordia will be evolved. In the present study, Egyptian wheat and barley varieties were transformed using this method to evaluate its efficiency in transforming different genotypes of wheat and different species where this method has not been reported for barley transformation.…”

Section: Introductionmentioning

confidence: 99%

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Novel recombinant binary vectors harbouring Basta (bar) gene as a plant selectable marker for genetic transformation of plants

Nada

2016

Physiol Mol Biol Plants

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Genetic transformation is one of the most widely used technique in crop improvement. However, most of the binary vectors used in this technique, especially cloning based, contain antibiotic genes as selection marker that raise serious consumer and environmental concerns; moreover, they could be transferred to non-target hosts with deleterious effects. Therefore, the goal of this study was reconstruction of the widely used pBI121 binary vector by substituting the harmful antibiotic selection marker gene with a less-harmful selection marker, Basta (herbicide resistance gene). The generated vectors were designated as pBI121NB and pBI121CB, in which Basta gene was expressed under the control of Nos or CaMV 35S promoter, respectively. The successful integration of the new inserts into both the vectors was confirmed by PCR, restriction digestion and sequencing. Both these vectors were used in transforming Arabidopsis, Egyptian wheat and barley varieties using LBA4404 and GV3101 Agrobacterium strains. The surfactant Tween-20 resulted in an efficient transformation and the number of Arabidopsis transformants was about 6-9 %. Soaked seeds of wheat and barley were transformed with Agrobacterium to introduce the bacteria to the growing shoot apices. The percentage of transgenic lines was around 16-17 and 14-15 % for wheat and barley, respectively. The quantitative studies presented in this work showed that both LBA4404 and GV3101 strains were suitable for transforming Egyptian wheat and barley.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Wheat and barley were transformed according to Supartana et al 2006. Each seed was pierced with small needle to facilitate the introduction of the Agrobacterium cells to the shoot apex.…”

Section: Wheat and Barley Co-cultivationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel recombinant binary vectors harbouring Basta (bar) gene as a plant selectable marker for genetic transformation of plants

Nada

2016

Physiol Mol Biol Plants

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Plant Cell Culture – Present and Future

Dunwell

2010

Plant Cell Culture

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Plant cell culture has a long history of development and exploitation that has been reviewed many times [1]. There are also several monographs and textbooks that provide detailed discussion on specific techniques and their application [2, 3]. By necessity therefore, the present review is limited in scope and, for the most part, will focus on recent publications. The various sections below each concentrate on a specific aspect of the technology; these are followed by a summary section relating to commercialization. MicropropagationMicropropagation in vitro is well established as a method to propagate [4], preserve and transport germplasm of many species including horticultural [5-7], medicinal [8, 9] and woody [10,11] plants. It also reduces the risk of moving pathogens and insects with the germplasm owing to the inherent pathogen detection capabilities of aseptic cultures. Since this technology is usually limited to the multiplication of pre-existing meristems and does not involve the regeneration of plants from single cells or tissue, it will not be discussed in detail here.

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