Development of silk fibroin‑sodium alginate scaffold loaded silk fibroin nanoparticles for hemostasis and cell adhesion

Shen, Ying; Wang, Xinyu; Li, Binbin; Guo, Yajin; Dong, Kuo

doi:10.1016/j.ijbiomac.2022.05.064

Cited by 20 publications

(14 citation statements)

References 54 publications

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“…It has been reported that shifting of these bands is expected in the treated samples. In this study, the measured values for amide bonds of fibroin were obtained at 1621, 1519 and 1233 cm -1 in accordance of the literature (Bhattacharjee et al, 2013;Hofmann et al, 2014;Shen et al, 2022;Xie et al, 2022). In addition, it was determined that these values showed a shift according to the applied scaffolding production methods and composite composition (Xie et al, 2022).…”

Section: A B C D Figure 2 Ftir Spectrum Of Na-alginate and Ca-alginatesupporting

confidence: 78%

“…The results showed that the use of methanol has resulted in the conformation to β-sheet structure (Zhang & Mo, 2011;Shen et al, 2022;Xie et al, 2022).…”

Section: A B C D Figure 2 Ftir Spectrum Of Na-alginate and Ca-alginatementioning

confidence: 99%

“…Instead, silk II structured materials containing anti-parallel β-layers are preferred. Alcohols like that ethanol and methanol can be used for crystallization of the silk fibroin molecules (Bhattacharjee et al, 2013;Hofmann et al, 2014;Shen et al, 2022). It is thought that the crystallization mechanism is primarily in the form of the interaction of the polar alcohol, which can form hydrogen bonds, with the water in the environment, and as a result, hydrophobic amino acids such as alanine and glycine cluster to form the planar β-layer structure.…”

Section: A B C D Figure 2 Ftir Spectrum Of Na-alginate and Ca-alginatementioning

confidence: 99%

“…Although direct mixing was not applied to the polymers it was determined that fibroin interacted with Ca-Alginate and thus its layered structure was disrupted and its porosity increased (Xie et al, 2022). Shen et al (2022), in their study in which they examined the effects of sodium alginate nanoparticle uses on silk fibroinsodium alginate scaffolds, SEM images of fibroin-alginate scaffolds prepared by lyophilization are similar to our samples in terms of porosity (Shen et al, 2022). The literature supports that the layered areas in the CaAlg/Fb composite structure originate from fibroin.…”

Section: Evaluation Of Surface Morphologymentioning

confidence: 99%

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Production and Characterization of Bilayer Tissue Scaffolds Prepared with Different Alginate-Salts and Fibroin

ÇELİK

MOHAMED

Emin

2022

Gazi University Journal of Science Part A: Engineering and Innovation

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The presented study aimed to design and characterize bilayer Alginate/Fibroin scaffolds to provide faster and higher quality treatment of skin tissue losses with tissue engineering approach. In this context, it was tried to form the dermis and epidermis layers with alginate salts (sodium and calcium) and fibroin with a biomimetic approach, and it was aimed to determine the most suitable alginate salt-fibroin composite scaffold by trying different production methods. The optimum design was determined by macroscopic measurement and dimensional analysis of the scaffolds produced by four different methods and their chemical structures were controlled with FTIR. Among the produced scaffolds, calcium alginate/fibroin (CaAlg/Fb) scaffolds were determined to have the most suitable morphological and chemical structure. With further characterization, the pore distribution and size were examined by SEM analysis and it was determined that surface pore diameters vary from 30 µm to 300 µm which are suitable for cell settlement. The thermal stability of the structure was determined by thermal gravimetry, and the degradation rate was calculated from the thermograms. According to the TG analysis, decomposition of the CaAlg/Fb scaffolds occurs much faster with temperature than homo-biopolymeric (CaAlg and Fb) structures. As a result, it was found that bilayer CaAlg/Fb scaffolds were capable of forming full-thickness dermal and/or also osteochondral wound dressings both morphologically and structurally. It is recommended to perform the tissue forming ability of this scaffold structure by performing advanced biological analyzes.

show abstract

Section: A B C D Figure 2 Ftir Spectrum Of Na-alginate and Ca-alginatesupporting

confidence: 78%

“…The results showed that the use of methanol has resulted in the conformation to β-sheet structure (Zhang & Mo, 2011;Shen et al, 2022;Xie et al, 2022).…”

Section: A B C D Figure 2 Ftir Spectrum Of Na-alginate and Ca-alginatementioning

confidence: 99%

Section: A B C D Figure 2 Ftir Spectrum Of Na-alginate and Ca-alginatementioning

confidence: 99%

Section: Evaluation Of Surface Morphologymentioning

confidence: 99%

See 2 more Smart Citations

Production and Characterization of Bilayer Tissue Scaffolds Prepared with Different Alginate-Salts and Fibroin

ÇELİK

MOHAMED

Emin

2022

Gazi University Journal of Science Part A: Engineering and Innovation

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“…The high specific surface area and hierarchically porous structure of composite sponges could vastly improve the contact area with blood, accelerate hemostasis and absorb wound exudate, as well as promote trauma healing ( Yan et al, 2022 ). High liquid absorption capacity can effectively absorb tissue exudate and achieve rapid hemostasis ( Shen et al, 2022 ). As depicted in Figure 3D , the fluid absorption rate of the AC sponge was higher than that of the AG sponge, but not significantly different from that of the ACQ-1 and ACQ-2 sponges.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial quaternary ammonium chitosan/carboxymethyl starch/alginate sponges with enhanced hemostatic property for the prevention of dry socket

Deng

Wang

Zhang

et al. 2023

Front. Bioeng. Biotechnol.

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Tooth extraction commonly leads to postoperative wound bleeding, bacterial infection, and even the occurrence of dry socket. Therefore, developing a biomedical material with favorable antibacterial and excellent hemostatic properties to prevent the post-extraction dry socket is necessary. Herein, quaternary ammonium chitosan/ carboxymethyl starch/alginate (ACQ) sponges are developed via Ca2+ cross-linking, electrostatic interaction, and lyophilization methods. The results show that the bio-multifunctional sponges exhibit interconnected porous structures with significant fluid absorption rates and suitable water vapor transmission rates. In vitro cellular and hemolysis experiments indicate that the developed sponges have acceptable biocompatibility. Notably, the constructed sponges effectively inhibit the growth of E. coli, S. aureus, and C. albicans, as well as achieve rapid hemostasis in the mouse liver injury and mini-pig tooth extraction models by absorbing blood and promoting red blood cell adhesion. Thus, the created bio-multifunctional sponges show tremendous promise as a hemostatic material for wound management after tooth extraction.

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Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

Rahman,

Dip,

Nur

et al. 2024

Macro Materials & Eng

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Silk fibroin (SF), a natural protein derived from silkworms, has emerged as a promising biomaterial due to its biocompatibility, biodegradability, degradation rate, and tunable mechanical properties. This review delves into the intrinsic attributes of SF that make it an attractive candidate for scaffold development in tissue engineering and regenerative medicine. The distinctiveness of this comprehensive review resides in its detailed exploration of recent advancements in the fabrication techniques of SF‐based fibrous scaffolds, namely electrospinning, freeze‐drying, and 3D printing. An in‐depth analysis of these fabrication techniques is conducted to illustrate their versatility in customizing essential scaffold characteristics, such as porosity, fiber diameter, and mechanical strength. The article meticulously discusses process parameters, advantages, and challenges of each fabrication technique, highlighting the innovative advancements made in the respective field. Furthermore, the review goes beyond fabrication techniques to provide an overview of the latest biomedical applications and research endeavors utilizing SF‐derived scaffolds. From nerve regeneration and wound healing to drug delivery, bone regeneration, and vascular tissue engineering, the diverse applications underscore the versatility of SF in adopting various biomedical challenges. Finally, the article emphasizes the need for standardized characterization techniques, scalable manufacturing processes, and long‐term in vivo studies.

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Development of silk fibroin‑sodium alginate scaffold loaded silk fibroin nanoparticles for hemostasis and cell adhesion

Cited by 20 publications

References 54 publications

Production and Characterization of Bilayer Tissue Scaffolds Prepared with Different Alginate-Salts and Fibroin

Production and Characterization of Bilayer Tissue Scaffolds Prepared with Different Alginate-Salts and Fibroin

Antibacterial quaternary ammonium chitosan/carboxymethyl starch/alginate sponges with enhanced hemostatic property for the prevention of dry socket

Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

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