Development of short tandem repeat (STR) and derived cleaved amplified polymorphic (dCAPS) markers for distinguishing species and varieties of the genus Panax in Vietnam

Dinh, Xuan Tu; Mai, Xuan Cuong; Nguyen, Minh Ly

doi:10.1007/s10722-023-01557-0

Cited by 2 publications

(2 citation statements)

References 25 publications

(16 reference statements)

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“…If we have to study DNA of an individual, STR are termed to be the best forensic tool for the comparable analysis because of the variable nature of its region showing polymorphism. STR are classified on the basis of the length of the major repeated units as mono, di, tri, tetra, penta, and hexanucleotide repeats, and each STR has multiple alleles found to be present separately on variable chromosomes (Dinh et al, 2023). These positions of the STR, which are defined by the number of repeats as described, help in knowing the genetic makeup of a particular species.…”

Section: Str As Forensic Toolmentioning

confidence: 99%

Essence of Short Tandem Repeat (STR) as Potential Markers for DNA Profiling Paving a Way for Forensic Investigations- An Update

Loveleen,

Varruchi,

Rupal

et al. 2023

Intern. J. Zool. Invest.

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As we know that wildlife is one of the most important treasures of the planet earth, as it makes our nature more and more powerful in one way or another, and helps us build ownselves. But with increasing needs of humans and multiple necessities, it is very much getting affected one way or the other and also being constantly under threat of degradation. Do we have appropriate and foolproof measures to preserve the deterioration stature of wildlife? The answer is still negative. Thus, highly essential steps are needed to preserve them from being poached and to protect them. Poaching these days is getting to newer heights because of the need for fetching more powers. Such species are being targeted and it is becoming very difficult to catch the culprits and to identify them even at a smaller scale. To do such forensic identification for wildlife, one has to come forward with newer research and ideas so as to minimise the rate of degradation for each species, making each species important and full of quality, and to be saved. Thus, like a human DNA database, a database for wildlife should be prepared on the basis of species identification, and this can be done by making the Short Tandem Repeats (STR) marker, the main component of the database and registering the species. With all this data, each species' DNA is required to be saved with all its morphological and non-morphological characters, including phenotypic and genotypic characters as well.

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Section: Str As Forensic Toolmentioning

confidence: 99%

Essence of Short Tandem Repeat (STR) as Potential Markers for DNA Profiling Paving a Way for Forensic Investigations- An Update

Loveleen,

Varruchi,

Rupal

et al. 2023

Intern. J. Zool. Invest.

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“…Using phylogenetic analysis, we attempted to determine the evolutionary position of HSVM by including various closely related species. Further, we identified intraspecific cp variations in HSVM, leading to the development of a specialized derived cleaved amplified polymorphic sequence (dCAPS) marker that can be used to distinguish between cultivars [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Insights into the Extremely Dwarf Hibiscus syriacus var. micranthus: Complete Chloroplast Genome Analysis and Development of a Novel dCAPS Marker

Kwon,

Shin

2024

CIMB

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This study explored the chloroplast (cp) genomes of three Hibiscus syriacus (HS) specimens endemic to Korea possessing unique ornamental and conservation values: the dwarf H. syriacus var. micranthus (HSVM), renowned for its small stature and breeding potential; HS ‘Tamra’, a cultivar from Korea’s southernmost islands, noteworthy for its distinctive beauty; and HS Natural Monument no. 521 (N.M.521), a specimen of significant lifespan and height. Given the scarcity of evolutionary studies on these specimens, we assembled and analyzed their cp genomes. We successfully assembled genomes spanning 160,000 to 160,100 bp and identified intraspecific variants. Among these, a unique ATA 3-mer insertion in the trnL-UAA region was identified in HSVM, highlighting its value as a genetic resource. Leveraging this finding, we developed a novel InDel dCAPS marker, which was validated across 43 cultivars, enhancing our ability to distinguish HSVM and its derivatives from other HS cultivars. Phylogenetic analysis involving 23 Malvaceae species revealed that HSVM forms a clade with woody Hibiscus species, closely associating with N.M.520, which may suggest a shared ancestry or parallel evolutionary paths. This investigation advances our understanding of the genetic diversity in Korean HS and offers robust tools for accurate cultivar identification, aiding conservation and breeding efforts.

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Development of short tandem repeat (STR) and derived cleaved amplified polymorphic (dCAPS) markers for distinguishing species and varieties of the genus Panax in Vietnam

Cited by 2 publications

References 25 publications

Essence of Short Tandem Repeat (STR) as Potential Markers for DNA Profiling Paving a Way for Forensic Investigations- An Update

Essence of Short Tandem Repeat (STR) as Potential Markers for DNA Profiling Paving a Way for Forensic Investigations- An Update

Genetic Insights into the Extremely Dwarf Hibiscus syriacus var. micranthus: Complete Chloroplast Genome Analysis and Development of a Novel dCAPS Marker

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