Development of separation materials using controlled/living radical polymerization

Wang, Huaisong; Dong, Xinyong; Yang, Meixian

doi:10.1016/j.trac.2011.07.012

Cited by 35 publications

(21 citation statements)

References 73 publications

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“…Development of separation materials via controlled living radical polymerization was reviewed by Wang et al. . Materials of various functional coatings were introduced .…”

Section: Introductionmentioning

confidence: 99%

C₁₈‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

Ali

Cheong

2014

J of Separation Science

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Ground porous silica monolith particles with an average particle size of 2.34 μm and large pores (363 Å) exhibiting excellent chromatographic performance have been synthesized on a relatively large scale by a sophisticated sol-gel procedure. The particle size distribution was rather broad, and the d(0.1)/d(0.9) ratio was 0.14. The resultant silica monolith particles were chemically modified with chlorodimethyloctadecylsilane and end-capped with a mixture of hexamethyldisilazane and chlorotrimethylsilane. Very good separation efficiency (185,000/m) and chromatographic resolution were achieved when the C18 -bound phase was evaluated for a test mixture of five benzene derivatives after packing in a stainless-steel column (1.0 mm × 150 mm). The optimized elution conditions were found to be 70:30 v/v acetonitrile/water with 0.1% trifluoroacetic acid at a flow rate of 25 μL/min. The column was also evaluated for fast analysis at a flow rate of 100 μL/min, and all the five analytes were eluted within 3.5 min with reasonable efficiency (ca. 60,000/m) and resolution. The strategy of using particles with reduced particle size and large pores (363 Å) combined with C18 modification in addition to partial-monolithic architecture has resulted in a useful stationary phase (C18 -bound silica monolith particles) of low production cost showing excellent chromatographic performance.

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“…Development of separation materials via controlled living radical polymerization was reviewed by Wang et al. . Materials of various functional coatings were introduced .…”

Section: Introductionmentioning

confidence: 99%

C₁₈‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

Ali

Cheong

2014

J of Separation Science

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“…It is difficult to control the propagation of individual polymer chains from initiation to termination in the process of free radical polymerization, resulting in the formation of microgels containing polymer chains with a heterogeneous distribution and a heterogeneous network structure. Controlled/living radical polymerization (CRP) has exhibited great potential in polymer synthesis and modification, owning to production of the polymers with the desired molecular weight and monodispersity . Compared with conventional free radical polymerization, ATRP would facilitate the formation of the 3D bicontinuous skeleton macroporous structure of polymeric monolith, attributing to the step‐growth of polymer chains .…”

Section: Basic Strategy For Designing Monolithic Materialsmentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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“…CRP has been used in molecular imprinting research. Its advantages have been demonstrated by different published results [14,15]. Reversible addition-fragmentation chain-transfer (RAFT) polymerization is a type of CRP with merits of wide application and mild reaction conditions [16].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of tetracycline-imprinted polymer microspheres by reversible addition-fragmentation chain-transfer precipitation polymerization using polyethylene glycol as a coporogen

Liu

Han

et al. 2014

J. Sep. Science

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Tetracycline (TC)-imprinted microspheres have been synthesized by reversible addition-fragmentation chain-transfer precipitation polymerization using PEG as a coporogen. In the synthesis, methacrylic acid and ethylene dimethacrylate were used as the functional monomer and cross-linker, respectively. 2,2'-Azobisisobutyronitrile was the initiator, and cumyl dithiobenzoate was the chain-transfer reagent. Although monodispersed microspheres were obtained using acetonitrile as porogen, the particles cannot be used in the column extraction because of the high backpressure. To increase the porosity of the material, PEG was introduced as a coporogen. The influence of the molecular weight and concentration of PEG on the morphology, binding affinity, and porosity of the molecularly imprinted polymers (MIPs) have been studied. The results demonstrated that PEG as a macroporogen increased the porosity of the polymers. Meanwhile, the column backpressure was reduced using the MIPs with higher porosity. The binding affinity of the MIPs was increased when a low concentration of PEG was employed, while it was decreased when the ratio of PEG 12,000/monomers was >0.8%. Under the optimized conditions, TC-imprinted microspheres with good selectivity and size uniformity have been obtained, which facilitates its application in the column extraction for TC determinations.

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Development of separation materials using controlled/living radical polymerization

Cited by 35 publications

References 73 publications

C₁₈‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

C₁₈‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Synthesis of tetracycline-imprinted polymer microspheres by reversible addition-fragmentation chain-transfer precipitation polymerization using polyethylene glycol as a coporogen

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Development of separation materials using controlled/living radical polymerization

Cited by 35 publications

References 73 publications

C18‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

C18‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Synthesis of tetracycline-imprinted polymer microspheres by reversible addition-fragmentation chain-transfer precipitation polymerization using polyethylene glycol as a coporogen

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Resources

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C₁₈‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

C₁₈‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance