Development of screening techniques to evaluate response of jute (Corchorus olitorius) to Macrophomina phaseolina

Mandal, Kunal; Kumar, Amrendra; Ghosh, D. C.

doi:10.1007/s13313-021-00798-w

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…The pathogenicity of these isolates was established by inoculating 70‐ to 90‐day‐old jute (cv. JRO‐524) plants under field conditions using the stem inoculation methods described by Mandal et al (2021). In brief, at the mid‐length of a selected plant, the bark epidermis was wounded with a sterile scalpel.…”

Section: Methodsmentioning

confidence: 99%

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Stem rot of jute (Corchorus spp.): New insight on its causal organisms

2022

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Stem rot disease is a major threat to the successful cultivation of jute (Corchorus spp.). Although Macrophomina phaseolina is the causal agent attributed to this disease, in the present study we documented the involvement of several pathogens. Disease samples were collected from West Bengal, a major jute‐growing state in India and six different types of symptoms were observed under field conditions. Isolated pathogens (n = 387) produced four types of colonies resembling growth patterns of Colletotrichum, Diaporthe, Lasiodiplodia and Macrophomina. Based on pigmentation on the reverse side of plates, Diaporthe‐ and Lasiodiplodia‐type colonies formed two subgroups each. However, no correlation could be established between types of disease symptoms and the associated pathogens. While Colletotrichum was prevalent in the Coochbehar district, the spatial distribution of the other pathogens was random. Internal transcribed spacer (ITS) of rDNA and translation elongation factor 1‐α (TEF) genes from two representative isolates from each of six morphogroups were amplified and sequenced. Phylogenetic trees were generated based on these sequences, which suggested that seven fungal pathogens belonging to four genera (Colletotrichum, Diaporthe, Lasiodiplodia and Macrophomina) were involved with stem lesions of jute. Three recommended fungicides (carbendazim, hexaconazole and tebuconazole) were tested against the selected fungal pathogens and all were found effective in suppressing their growth in vitro.

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Section: Methodsmentioning

confidence: 99%

“…The pathogenicity of these isolates was established by inoculating 70-to 90-day-old jute (cv. JRO-524) plants under field conditions using the stem inoculation methods described by Mandal et al (2021).…”

Section: Isolation and Preservation Of Pathogensmentioning

confidence: 99%

Stem rot of jute (Corchorus spp.): New insight on its causal organisms

2022

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“…Environmental factors, like cloudy weather, abundant rainfall, high atmospheric humidity and low soil temperature (<30°C), favour infection. The pathogen remains dormant in soil amidst crop debris (Mandal et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of F2 Mapping Population for Stem Rot Tolerance in Tossa Jute (Corchorus olitorius)

Meena,

Bhandari,

Mangal

et al. 2024

IJBSM

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Present investigation was undertaken at ICAR-CRIJAF, Barrackpore, West Bengal, India during summer season (Apr–July) of 2020 to identify the jute breeding lines resistant to stem rot which is one of the major problems in jute production. The identified resistant and susceptible jute accessions (RS-6 and OIJ-272, respectively) were crossed to develop a mapping population for the disease. The resultant F2 population comprising 125 plants was screened for resistance to stem rot disease caused by Macrophomina phaseolina following artificial inoculation. The progress of infection was measured as the length of the infected stem portion (i.e., lesion length in cm) after 7, 14 and 21 days of inoculation (time replication). After stem inoculation investigation indicated that in the F2 population of the cross OIJ-272×RS-6, only 14 plants (PL-100, PL-40, PL-99, PL-33, PL-89, PL-119, PL-1, PL-43, PL-118, PL-121, PL-6, PL-11, PL-55 and PL-86) were found moderately resistant (lesion length 2.0–3.0 cm). In contrast, 10 plants (PL-4, PL-31, PL-19, PL-27, PL-3, PL-50, PL-18, PL-26, PL-47, PL-51) were found highly susceptible (lesion length >5.0 cm). The remaining 101 plants exhibited moderate susceptible symptoms. While the parent OIJ-272 exhibited high susceptibility to stem rot with an average lesion length of 5.53 cm, the parent RS-6 exhibited moderate resistant reaction with an average lesion length of 2.30 cm. Because of good resistance under artificial stem inoculation of this fungi, these 14 F2 plants of the cross can be further exploited for a resistance breeding programme against this deadly disease.

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