Development of SCAR markers to distinguish male-sterile and normal cytoplasm in bunching onion (<i>Allium fistulosum</i>L.)

Gao, Limin; Chen, Y. Q.; Huo, Yumeng; Ding, Fan; Yang, Yayun; Kong, Shu; Chen, W.; Xiong, Wei

doi:10.1080/14620316.2015.11513153

Cited by 6 publications

(4 citation statements)

References 1 publication

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“…Gao et al. () developed SCAR markers to distinguish male‐sterile cytoplasm from normal cytoplasm in bunching onion. In soybean, Zhang et al.…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al (2012) used mitochondrial SCAR and SSR markers to distinguish cytoplasmic male-sterile lines from their isogenic maintainer lines in cotton. Gao et al (2015) developed SCAR markers to distinguish male-sterile cytoplasm from normal cytoplasm in bunching onion. In soybean, Zhang et al (2013) used a SCAR marker to distinguish CMS lines from maintainer lines, and Lin et al (2014) developed some markers from chloroplast genomes to classify soybean cytoplasm sources.…”

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confidence: 99%

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Development of a cytoplasmic male‐sterile line NJCMS4A for hybrid soybean production

et al. 2017

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Cytoplasmic male‐sterile (CMS) lines are being used to produce hybrid seeds. Thus far, four CMS sources in soybean [Glycine max (L.) Merr.] have been reported in China. However, they are not sufficient or efficient in meeting the requirements of commercial soybean hybrid seed production. In this study, 33 varieties were tested for CMS using 45 crosses among 37 landraces and 17 annual wild soybean accessions (Glycine soja Sieb. et Zucc.). The cross of N23661 × N23658 showed partial to complete male sterility in backcross generations, while the corresponding reciprocal cross showed normal male fertility. Thus, the cytoplasm of N23661 is male‐sterile, the continuously backcrossed line is a male‐sterile line (designated NJCMS4A), and N23658 is its maintainer (designated NJCM4B). The male fertility of NJCMS4A was restored by another accession, Nansheng9403. Accordingly, NJCMS4A along with its maintainer and restorer composes a complete set of three lines for producing hybrid soybean. Using mitochondrial markers and sequence analyses, NJCMS4A is a CMS line with its cytoplasm not identical to the four previously reported CMS sources in soybean.

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“…Gao et al. () developed SCAR markers to distinguish male‐sterile cytoplasm from normal cytoplasm in bunching onion. In soybean, Zhang et al.…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Development of a cytoplasmic male‐sterile line NJCMS4A for hybrid soybean production

et al. 2017

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“…Mitochondria are one of the most plant cell organelles and have been suggested to play a very important role in plant defenses (Gleason et al, 2011;Laluk et al, 2011;Ambrosio et al, 2013;Qamar et al, 2015;Fuchs et al, 2016). Furthermore, mitochondrial DNA (mtDNA) is also widely used as molecular markers in the fields of systematic evolution, cytoplasmic male sterility and so on (Ji et al, 2014;Gao et al, 2015;Shu et al, 2016). However, to date, there have been no reports about the presence of plant disease resistance or susceptibility-related SCAR markers in mitochondrial genome yet.…”

Section: Cloning and Sequencing Of Srap Productsmentioning

confidence: 99%

Development of a Mitochondrial SCAR Marker Related to Susceptibility of Banana (Musa AAA Cavendish) to Fusarium oxysporum f. sp. Cubense Race 4

Fang¹,

Lan²,

Lv³

et al. 2018

Not Bot Horti Agrobo

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The use of resistant cultivars is an effective method for the control of banana (Musa spp.) Fusarium wilt caused by race 4 of Fusarium oxysporum f. sp. cubense (Foc4). However, selection of disease-resistant cultivars requires large-scale field evaluations and is time-consuming. Development of early, reliable, and reproducible selection strategies can speed up this process. Sequence characterized amplified region (SCAR) markers have been widely employed in the resistant breeding of many crops. However, to date, there have been no reports about the presence of plant disease resistance-related SCAR markers in mitochondrial genome yet, which also plays a very important role in plant defenses. In the present study, a sequence-related amplified polymorphism (SRAP) marker, a specific fragment of 829 bp, was identified. This fragment could be amplified from Foc4-susceptible but not from the resistant cultivars. It was located in banana mitochondrial genome and mapped near the putative cytochrome c biogenesis ccmB-like mitochondrial protein. This fragment was then successfully converted into a SCAR marker, namely Mito-Foc-S001, which was found to be able to discriminate the resistance from susceptibility to Fusarium wilt disease of bananas with the discriminatory power of the new mark being 96.88%. Thus, this marker can be used in banana (Musa AAA Cavendish) breeding for Fusarium wilt disease resistance.

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“…Additionally, intraspecific crosses and subsequently backcrossing schemes will incorporate well known disease resistance from other species such as A. cepa [10,11]. Finally, the incorporation of cytoplasmic male sterility (CMS) using marker assisted selection [27] will accelerate the hybrid evaluation and trait introgression for new varieties.…”

Section: Genotype × Environment Interaction (G × E)mentioning

confidence: 99%

Agronomic Evaluation of Bunching Onion in the Colombian Cundiboyacense High Plateau

Galeano-Mendoza

Cubillos

Varón

et al. 2018

International Journal of Agronomy

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Bunching onion (Allium fistulosum L.) is a strategic crop for Colombia due to its economic relevance within fresh and processed food markets, and therefore, there is a demand for high yielding genotypes adapted to specific regions. For this reason, after carrying out a clonal selection process including 62 genotypes, ten of these, including a regional control, were evaluated for six different traits in Boyacá (Colombia) during 2012 and 2013. These traits were grouped into agronomic, yield, and processing categories. In general, these showed significant differences ( ≤ 0.01) for genotypes, location, and genotypes × location interaction. Compared with the regional control and based on the multienvironmental analysis the genotypes Clone 30 and Clone 38 were the most promising new cultivars identified in this study. These two clones showed comparative advantages on earliness and yield, and they moreover showed some level of resistance to downy mildew and root rot, the most limiting diseases for Boyacá's bunch onion farmers. Therefore, Clone 30 and Clone 38 were registered as new bunching onion cultivars for the Cundiboyacense High Plateau region under the names Corpoica Aquitania-1 and Corpoica Tota-1, respectively. Finally, further approaches and initiatives on bunching onion breeding are discussed.

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Development of SCAR markers to distinguish male-sterile and normal cytoplasm in bunching onion (Allium fistulosumL.)

Cited by 6 publications

References 1 publication

Development of a cytoplasmic male‐sterile line NJCMS4A for hybrid soybean production

Development of a cytoplasmic male‐sterile line NJCMS4A for hybrid soybean production

Development of a Mitochondrial SCAR Marker Related to Susceptibility of Banana (Musa AAA Cavendish) to Fusarium oxysporum f. sp. Cubense Race 4

Agronomic Evaluation of Bunching Onion in the Colombian Cundiboyacense High Plateau

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