Development of SCAR Markers Linked to L3 gene in Capsicum

Abstract

Random amplified polymorphic DNA (RAPD) markers linked to the L 3 locus were developed by applying the bulked segregant analysis method to two doubled haploid (DH) populations in Capsicum. The codominant RAPD markers, E18 272 and E18 286 , were converted into sequence-characterized amplified region (SCAR) markers by molecular cloning and nucleotide sequencing. A PCR analysis using DH (n = 176) and backcross (n = 190) populations revealed that all the SCAR markers, PMFR11 269 , PMFR11 283 and PMFR21 200 , co-segregated with the original RAPD markers, and were mapped at a distance of 4.0 cM from the L 3 locus. Furthermore, after confirmation of their validity in 18 accessions of Capsicum spp. using the codominant SCAR marker pair, PMFR11 269 and PMFR11 283 , it was suggested that the SCAR markers developed here could become effective in marker-assisted selection programs for the introduction of the L 3 gene derived from PI159236 (C. chinense) into sweet pepper for breeding purposes.

QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

“…'K9-11' harbors the L 3 gene, which is resistant to pepper mild mottle virus (PMMoV) derived from PI 159236. This non-pungent bell-shaped sweet pepper is susceptible to P. capsici (Yanokuchi et al 1993, Sugita et al 2004. 'AC2258' is highly resistant to P. capsici and is pungent (Smith et al 1967, Yamakawa et al 1979, Palloix et al 1990).…”

“…In the Capsicum species, several linkage maps have been reported (Prince et al 1993, Lefebvre et al 1995, Livingstone et al 1999, Kang et al 2001. Thus, DNA markers linked to various agronomic characters were developed (Caranta et al 1999, Moury et al 2000, Blum et al 2002, Matsunaga et al 2003, Sugita et al 2004. These DNA markers are now available for MAS for some traits.…”

Rapid Construction of a Linkage Map using High-efficiency Genome Scanning/AFLP and RAPD, Based on an Intraspecific, Doubled-haploid Population of Capsicum annuum

“…'K9-11' harbors the L 3 gene derived from PI159236, and is a bell-like sweet pepper (Yanokuchi et al 1993, Sugita et al 2004. It is susceptible to phytophthora blight and has no pungency, and its immature fruit color is dark green.…”

“…An RFLP marker TG36 using a tomato probe, was mapped at 4.0 cM from L 3 with an interspecific DH population by Lefebvre et al (1995). The SCAR markers, PMFR11 269 , PMFR11 283 and PMFR21 200 were mapped at 4.0 cM from L 3 with an intraspecific DH population by Sugita et al (2004) using a bulked segregant analysis method. A SCAR marker, WA31-1500S, was mapped at 1.5 cM from L 4 with an intraspecific F 2 population by Matsunaga et al (2003).…”

“…These markers include disease resistance to pepper mild mottle virus (PMMoV; L 4 ) (Matsunaga et al 2003), cucumber mosaic virus (Ben et al 2001a), tomato spotted wilt virus (Moury et al 2000) and potato virus Y (Caranta et al 1999), fruit shape (Ben et al 2001b), pungency (Blum et al 2002) and mature fruit color (Popovsky andParan 2000, Huh et al 2001). Moreover, we have developed SCAR markers linked to PMMoV resistance (L 3 ) (Sugita et al 2004).…”