Development of salt-tolerance interface for an high performance liquid chromatography/inductively coupled plasma mass spectrometry system and its application to accurate quantification of DNA samples

Separation and quantification of RNA molecules using size‐exclusion chromatography hyphenated with inductively coupled plasma‐mass spectrometry

“…We have reported the coupling techniques of LC with ICP‐MS, where we employed RP chromatography only for the analysis of mono‐nucleotides was performed . As an application for DNA quantification, we have also reported ion‐exchange chromatography hyphenated with ICP‐MS, though we could not apply this method to the RNA analysis, since the ionic strength of RNA was higher than that of DNA . Traditionally, microchip gel electrophoresis or CGE using a modified capillary filled with agarose or polyacrylamide is applied to the separation of RNA fragments.…”

Recent developments in the characterization of nucleic acids by liquid chromatography, capillary electrophoresis, ion mobility, and mass spectrometry (2010–2020)

“…A weak anion‐exchange monolithic column coupled to ICP‐MS was utilized for the separation and quantitation of DNA fragments and oligodeoxythymidilic acids (dT12‐18) based on detection of phosphorus. For this study, a capillary‐attached micronebulizer was designed to interface the LC system to the ICP‐mass spectrometer and allow lower sample consumption and salt tolerance [123]. Anion exchange was also used in mixed mode in conjunction with reversed phase chromatography via a surface‐bonded N‐11‐undecenyl‐3‐aminoquinuclidine stationary phase based on 5 μm thiol‐silica for the separation of synthetic oligonucleotides with minor sequence variations.…”

Arsenic speciation and its DNA fractionation in the rice plant Oryza sativa

“…One possible explanation is that this is because of DNA being such a large molecular species with many negative charges present, arising from the presence of phosphate groups in its backbone. 30 These may become bound very strongly with the stationary phase of the column and not easily be eluted. Therefore the sum of As species in the chromatographic separation from the injected DNA extracts using HPLC-ICP-MS was lower than the total As measured from the extracts of DNA measured using ICP-MS. As a result one can hypothesise that these As 'species' which can be detected on the chromatogram are (i) only relatively weakly associated with the already well washed DNA molecules (because they can easily be removed from the DNA itself using only competitive sulfate ions) and (ii) that some of the inorganic As V made freely available during the extraction procedure may have become associated with the DNA despite intensive washing.…”