Development of rolled scaffold for high-density adherent cell culture

YekrangSafakar, Ashkan; Hamel, Katie; Mehrnezhad, Ali; Jung, Jangwook P.; Park, Kidong

doi:10.1007/s10544-019-0459-9

Cited by 9 publications

(10 citation statements)

References 51 publications

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“…We compared harvested cell counts to the final OCR total value just before harvesting cells to identify the correlation between CHO‐K1 cell count and oxygen consumption in the RS bioreactor. The data presented in Figure 3c was collected from multiple cell expansions in UV‐RS bioreactors in our previous study (YekrangSafakar et al, 2020) and Mesh‐RS bioreactors in this article. The average cell‐specific oxygen uptake rate (OUR CHO‐K1 ) was 39.2 ± 3.5 amol/cell/s, which is similar to the reported values of 55 amol/cell/s (Gray et al, 1996), and 47–66.8 amol/cell/s (Goudar et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Rolled scaffold (RS), developed in our earlier study (YekrangSafakar et al, 2020), is a platform for the high-density adherent culture of mammalian cells. The RS is fabricated by rolling polymer film around a center rod to make spiraled layers in a cylindrical form, as shown in Figure 1a.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, the RS structure was redesigned for enhanced manufacturability, and a dialysis culture based on the RS bioreactor was demonstrated. In our earlier publication (YekrangSafakar et al, 2020), the first generation of RS, termed as UV-RS in this report, was fabricated through manual UV-imprinting, making it difficult to manufacture largescale bioreactors. To overcome this issue, we developed Mesh-RS and Fiber-RS, both of which used a feed spacer to control the gap between each layer, as shown in Figure 2a.…”

Section: Introductionmentioning

confidence: 99%

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High‐density adherent culture of CHO cells using rolled scaffold bioreactor

YekrangSafakar

Mehrnezhad

et al. 2022

Biotech & Bioengineering

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Rapid expansion of biopharmaceutical market calls for more efficient and reliable platforms to culture mammalian cells on a large scale. Stirred-tank bioreactors have been widely used for large-scale cell culture. However, it requires months of trials and errors to optimize culture conditions for each cell line. In this article, we extend our earlier studies on rolled scaffold (RS) bioreactors for high-density adherent cell culture and report two new implementations of RSs with greatly enhanced massmanufacturability, termed as Mesh-RS and Fiber-RS. CHO-K1 cells were successfully expanded in Mesh-RS and Fiber-RS bioreactors with an average growth rate of 1.09 ± 0.04 1/day and 0.95 ± 0.07 1/day, which were higher than those reported in similar studies. Fiber-RS bioreactor exhibited a very high cell density of 72.8 × 10 6 cells/ml. Besides, a dialyzer was integrated into the RS bioreactor to remove cellular waste and to replenish nutrients without disturbing the cells. By collecting the dialyzed media separately, the dialysis efficiency was significantly improved. In conclusion, the developed RS bioreactor has a strong potential to provide a highly reliable and easily scalable platform for large-scale cell culture in the biopharmaceutical industry.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High‐density adherent culture of CHO cells using rolled scaffold bioreactor

YekrangSafakar

Mehrnezhad

et al. 2022

Biotech & Bioengineering

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Section: Methodsmentioning

confidence: 99%

“…To prevent evaporation, each well was covered with parafilm and the entire plate was wrapped with parafilm. A polymer optical fiber (POF-L2.5-2SMA, PreSens, Regensburg, Germany) optically senses the concentration of O2 through the bottom of a well and sends signals to an O2 sensor (OXY-1 SMA, PreSens, Regensburg, Germany) 29 for measurement. Swelling ratio and in vitro degradation of lignin composites Swelling ratios of lignin composites were determined by the following equation: [Ws-Wd]/Wd, where Ws and Wd represent the weight after swelling in PBS for 24 h and the weight after lyophilization, respectively.…”

Section: Quantification Of O2 Release From Lignin Compositesmentioning

confidence: 99%

Engineering Antioxidant and Oxygen-Releasing Lignin Composites to Promote Wound Healing

Balaji

Short

Farouk

et al. 2022

Preprint

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The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignosulfonates to confer antioxidation to tissue microenvironments and to deliver oxygen to accelerate vascularization and healing responses without causing inflammatory responses. The results from fibrosis array shows that thiolated lignosulfonate in methacrylated gelatin can effectively attenuate fibrotic responses of human dermal fibroblasts. Elemental analysis of oxygen releasing nanoparticles shows the positive incorporation of calcium peroxide. Composites including nanoparticles of lignosulfonate and calcium peroxide release around 0.05% oxygen per day at least for 7 days. Stiffness can be precisely modulated to avoid adverse inflammatory responses. Injection of lignin composites with oxygen generation nanoparticles enhanced the formation of blood vessels and promoted infiltration of alpha-smooth muscle actin+ fibroblasts over 7 days. At 30 days after surgery, the lignin composite with oxygen generating nanoparticles remodels the collagen architecture resembling to the reticular pattern of normal collagen and leave minimal scars. Thus, our study shows the potential of functionalized lignosulfonate for wound healing applications requiring balanced antioxidation and controlled release of oxygen.

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Magnetically Actuated GelMA‐Based Scaffolds as a Strategy to Generate Complex Bioprinted Tissues

Ergene,

Liman,

Yilgor

et al. 2024

Adv Materials Technologies

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The 3D bioprinting of complex structures has attracted particular attention in recent years and has been explored in several fields, including dentistry, pharmaceutical technology, medical devices, and tissue/organ engineering. However, it still possesses major challenges, such as decreased cell viability due to the prolongation of the printing time, along with difficulties in preserving the print shape. The 4D bioprinting approach, which is based on controlled shape transformation upon stimulation after 3D bioprinting, is a promising innovative method to overcome these difficulties. Herein, the generation of skeletal muscle tissue‐like complex structures is demonstrated by 3D bioprinting of GelMA‐based C2C12 mouse myoblast‐laden bio‐ink on a polymeric magnetic actuator that enables on‐demand shape transformation (i.e., rolling motion) under a magnetic field. Bioprinted scaffolds are used in both unrolled (open as control) and rolled forms. The results indicate that C2C12s remain viable upon controlled shape transformation, and functional myotube formation is initiated by the 7th day within bioprinted platforms. Moreover, when the rolled and open groups are compared regarding MyoD1 staining intensity, the rolled one enhanced MyoD1 expression. These results provide a promising methodology for generating complex structures with a simple magnetic actuation procedure for the bioprinting of tissue‐engineered constructs with enhanced cell viability and functionality.

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Development of rolled scaffold for high-density adherent cell culture

Cited by 9 publications

References 51 publications

High‐density adherent culture of CHO cells using rolled scaffold bioreactor

High‐density adherent culture of CHO cells using rolled scaffold bioreactor

Engineering Antioxidant and Oxygen-Releasing Lignin Composites to Promote Wound Healing

Magnetically Actuated GelMA‐Based Scaffolds as a Strategy to Generate Complex Bioprinted Tissues

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