Development of rice as potential carriers for probiotic <i>Lactobacillus amylovorus</i>

Chumphon, Thapakorn; Sriprasertsak, Permpong; Promsai, Saran

doi:10.1111/ijfs.13079

Cited by 14 publications

(11 citation statements)

References 17 publications

(13 reference statements)

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“…The gut microbiota have physiological functions associated with immune system and maintenance of intestinal homeostasis. A recent study showed that LP rice had a high survival rate of probiotic bacteria compared with other Thai rice cultivars [26] and black rice also had prebiotic activity in regulating the intestinal environment [27]. Thus, the current authors are now evaluating the effects of LP extract on the changing of microbiota in guts of IBD in animal models.…”

Section: Discussionmentioning

confidence: 99%

Dark Purple Luem Pua rice extract reduces disease severity in rat models of inflammatory bowel disease

Thipart¹,

Phunikhom²,

Noenplab³

et al. 2020

Trop. J. Pharm Res

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Purpose: To investigate the effects of an aqueous extract of unpolished dark purple glutinous Thai rice, variety Luem pua (LP), in two rat models of inflammatory bowel disease (IBD). Methods: Polyphenolic compounds content were determined by HPLC methods and antioxidant activities by DPPH and FRAP assays of the LP extract. The effect of the LP extract at 5 g/kg/day were evaluated in two rat models of IBD that included acetic acid and indomethacin induced IBD. On each day of treatment, changes of body weight, stool consistency and stool blood were scored and expressed as disease activity index (DAI). At the end of the experiments, the animals were euthanized. Colon length and spleen weight were determined, and the degree of inflammation of the colon was scored. Results: Rats in both models of IBD (acetic acid- and indomethacin-induced IBD), exhibited significant increases in DAI, macroscopic inflammation scores and spleen weights, while the lengths of colon were decreased. Pretreatment with LP extract attenuated the disease severity in both models as seen by the reverse of all observed altered parameters. Conclusion: These data suggest that LP extract might be beneficial in preventing and/or treating IBD. Keywords: Inflammatory bowel disease (IBD), Dark purple glutinous rice Var. Luem Pua, Acetic acid, Indomethacin

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Section: Discussionmentioning

confidence: 99%

Dark Purple Luem Pua rice extract reduces disease severity in rat models of inflammatory bowel disease

Thipart¹,

Phunikhom²,

Noenplab³

et al. 2020

Trop. J. Pharm Res

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“…It would be interesting to test the affinity of this same strain collection to other food components in future work, in order to dispose of more comparison points to our study and to get a better understanding of the importance of adhesion to β-lactoglobulin compared to adhesion to other food components. Currently, the rare existing studies discussing bacterial adhesion to food components other than β-lactoglobulin concern up to four strains at most at a time (De Bellis et al, 2010; Chumphon et al, 2016; Tarazanova et al, 2017, 2018a,b; Utratna et al, 2017), therefore failing to provide an overview of adhesion to food components amongst wide bacterial groups such as the LAB group.…”

Section: Discussionmentioning

confidence: 99%

“…However, the impact and technological interest of adhesive interactions is yet poorly documented and largely remains to be investigated (Hickey et al, 2015). Adhesive interactions between bacterial surface components and dairy components have only been studied for very few wild type strains, namely LGG (Guerin et al, 2016), Lactobacillus amylovorus (Chumphon et al, 2016), and Lactobacillus paracasei (De Bellis et al, 2010). This article goes one step forward in that direction by applying the high-throughput screening method recently developed by Gomand et al (2018) to a collection of 73 LAB strains (for which genome sequence is available) in order to characterize their potential adhesive behavior toward the major dairy protein β-lactoglobulin, to which the adhesive behavior of the model strain LGG is already well-known (Burgain et al, 2013b, 2014b, 2015; Guerin et al, 2016, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Adhesive Interactions Between Lactic Acid Bacteria and β-Lactoglobulin: Specificity and Impact on Bacterial Location in Whey Protein Isolate

et al. 2019

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In the last decade, there has been an increasing interest in the potential health effects associated with the consumption of lactic acid bacteria (LAB) in foods. Some of these bacteria such as Lactobacillus rhamnosus GG (LGG) are known to adhere to milk components, which may impact their distribution and protection within dairy matrices and therefore is likely to modulate the efficiency of their delivery. However, the adhesive behavior of most LAB, as well as its effect on food structuration and on the final bacterial distribution within the food matrix remain very poorly studied. Using a recently developed high-throughput approach, we have screened a collection of 73 LAB strains for their adhesive behavior toward the major whey protein β-lactoglobulin. Adhesion was then studied by genomics in relation to common bacterial surface characteristics such as pili and adhesion-related domain containing proteins. Representative adhesive and non-adhesive strains have been studied in further depth through biophysical measurement using atomic force microscopy (AFM) and a relation with bacterial distribution in whey protein isolate (WPI) solution has been established. AFM measurements have revealed that bacterial adhesion to β-lactoglobulin is highly specific and cannot be predicted accurately using only genomic information. Non-adhesive strains were found to remain homogeneously distributed in solution whereas adhesive strains gathered in flocs. These findings show that several LAB strains are able to adhere to β-lactoglobulin, whereas this had only been previously observed on LGG. We also show that these adhesive interactions present similar characteristics and are likely to impact bacterial location and distribution in dairy matrices containing β-lactoglobulin. This may help with designing more efficient dairy food matrices for optimized LAB delivery.

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“…Most probiotic products are developed using Lactobacillus, Bifidobacterium, Bacillus such as Enterococcus and Weisella. At present, there is one strain of yeast (Saccharomyces boulardii) used as a probiotic (Chumphon et al, 2016;Azad et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Rice (Oryza sativa) is a principal food for more than one-half the world's population (Chumphon et al, 2016). In particular, many varieties of Thai-pigmented rice have been developed in many countries including Thailand where they are recognised for their nourishing phytochemical content, such as cultivar glutinous Luem Pua, Black Jasmine and Riceberry rice (Thipart et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Innovative production of multistrain synbiotic product using Thai‐pigmented rice and rice bran oil

Chumphon

Pangjit

Promsai

2021

Int J of Food Sci Tech

Self Cite

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This study found that the isolated probiotics Lactobacillus paracasei KUKPS6201, L. acidophilus KUKPS6107, L. reuteri KUKPS6103, L. rhamnosus KUKPS6007, L. salivarius KUKPS6202, Bacillus coagulans KPSTF02 and Saccharomyces boulardii KUKPS6005 had high potential for probiotic properties. All strains had antibacterial activity and high antioxidant activity of 1.654 AE 0.017 mg Trolox mL −1 probiotic extract. The selected strains could survive in a simulated gastrointestinal tract under anaerobic conditions and showed no haemolytic activity. Furthermore, the probiotic strains were strongly autoaggregated and also showed co-aggregated ability with pathogenic bacteria. The probiotic microorganisms demonstrated high ability to adhere to Thai-pigmented rice grains. The results of analysis of these probiotics showed that Riceberry rice bran oil was an excellent prebiotic. A synbiotic product containing Thaipigmented rice grains (cultivar Riceberry, Luem Pua and Black Jasmine) and rice bran oil was produced. After 8 weeks of storage, the viability of the probiotics in terms of multistrains was 7.36 AE 0.04 log CFU g -1 (85.78% survival rate). Microbiological safety testing indicated that the amounts of contaminants were acceptable. This study provided the first scientific report on the feasibility of applying Thai-pigment rice, rice bran oil and mixed-culture probiotics as a novel functional synbiotic product.

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Development of rice as potential carriers for probiotic Lactobacillus amylovorus

Cited by 14 publications

References 17 publications

Dark Purple Luem Pua rice extract reduces disease severity in rat models of inflammatory bowel disease

Dark Purple Luem Pua rice extract reduces disease severity in rat models of inflammatory bowel disease

Adhesive Interactions Between Lactic Acid Bacteria and β-Lactoglobulin: Specificity and Impact on Bacterial Location in Whey Protein Isolate

Innovative production of multistrain synbiotic product using Thai‐pigmented rice and rice bran oil

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