Development of rhamnose-rich hydrogels based on sulfated xylorhamno-uronic acid toward wound healing applications

Chen, Xifang; Yue, Zhilian; Winberg, Pia C.; Dinoro, Jeremy; Hayes, Peter C.; Beirne, Stephen; Wallace, Gordon G.

doi:10.1039/c9bm00480g

Cited by 25 publications

(27 citation statements)

References 38 publications

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“…The data extraction of the included studies is presented in Table 1 . The studies were classified depending on the study design, whereas; twelve studies were in vitro [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], two studies were in vivo [ 31 , 32 ], and four studies conducted both [ 33 , 34 , 35 , 36 ]. The characteristics and outcomes of both in vitro and in vivo studies are presented in Table 2 and Table 3 , respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Overall, the majority of in vitro studies used fibroblastic skin cells. Human dermal fibroblasts (HDFs) were commonly used [ 20 , 21 , 22 , 24 , 27 , 28 , 31 , 34 , 35 , 36 ], followed by T3T mouse fibroblasts [ 19 , 23 , 25 , 29 , 30 ], and L929 mouse fibroblasts [ 33 , 35 ]. However, human epidermal keratinocytes (HEKs) were also used in four studies [ 22 , 31 , 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…Various crosslinking methods were used, and only six studies [ 25 , 29 , 30 , 31 , 33 , 34 ] reported that no crosslinking agent was applied. The following techniques were used: (1) chemical crosslinking by Ca +2 [ 23 , 26 , 27 ], CaCl 2 [ 28 , 32 ], 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) [ 24 ], N-hydroxysuccinimide-1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC-NHS) [ 19 , 21 , 28 , 36 ], nebulized sodium bicarbonate (NaHCO 3 ) [ 22 ], 1,4-butanediol diglycidyl ether (BDDE) [ 27 ]; and (2) physical crosslinking by either UV light [ 20 , 23 , 35 ] or cooling [ 26 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…Even though significant changes in proliferation rate were not evident in three studies [ 19 , 22 , 35 ], they reported high cell viability. Seven studies reported good cell viability [ 20 , 22 , 24 , 25 , 29 , 30 , 33 ], five reported a minimum of 85.07–98% cell viabilities [ 22 , 25 , 26 , 29 , 34 ], and one reported some dead cells indicating low cell viability [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…GelMA is also known to have good biocompatibility, and of promoting cell to cell interaction and cell migration. Furthermore, the advantageous mechanical stability of GelMA after UV crosslinking was used to induce a high shape fidelity of natural-based bioinks, such as cellulose nanofibrils [ 23 ] and silk sericin [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

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Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Smandri

Nordin

et al. 2020

Polymers

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Three-dimensional bioprinting has rapidly paralleled many biomedical applications and assisted in advancing the printing of complex human organs for a better therapeutic practice. The objective of this systematic review is to highlight evidence from the existing studies and evaluate the effectiveness of using natural-based bioinks in skin regeneration and wound healing. A comprehensive search of all relevant original articles was performed based on prespecified eligibility criteria. The search was carried out using PubMed, Web of Science, Scopus, Medline Ovid, and ScienceDirect. Eighteen articles fulfilled the inclusion and exclusion criteria. The animal studies included a total of 151 animals with wound defects. A variety of natural bioinks and skin living cells were implanted in vitro to give insight into the technique through different assessments and findings. Collagen and gelatin hydrogels were most commonly used as bioinks. The follow-up period ranged between one day and six weeks. The majority of animal studies reported that full wound closure was achieved after 2–4 weeks. The results of both in vitro cell culture and in vivo animal studies showed the positive impact of natural bioinks in promoting wound healing. Future research should be focused more on direct the bioprinting of skin wound treatments on animal models to open doors for human clinical trials.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Smandri

Nordin

et al. 2020

Polymers

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3D Bioprinting Constructs to Facilitate Skin Regeneration

Daikuara

Chen

Yue

et al. 2021

Adv Funct Materials

Self Cite

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In the last decade, the advent of 3D printing for tissue engineering and regenerative medicine has engendered great interest for those involved in skin repair and regeneration. 3D bioprinting allows spatial distribution of skin cells into predefined custom‐made structures to produce living skin mimics on the bench for grafting or drug testing. The key aspect of 3D bioprinting lies in the formulation of printable bioinks serving as matrix mimics to house skin cells, alongside an appropriate combination of cells. In this review, bioink formulations, cell combinations, as well as manufacturing methods exploited to develop 3D bioprinted constructs for skin regeneration are summarized. Issues to do with the selection of suitable materials and cells to ensure the functionality of the resulting skin constructs and fabrication of skin appendages are also addressed.

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Manufacturing Ulvan Biopolymer for Wound Dressings

Foroughi,

Ruhparwar,

Aloko

et al. 2023

Macro Materials & Eng

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Although recent advancements in wound healing strategies have led to successful treatment for numerous wounds, the incidence of wound infections remain elevated, and is anticipated to rise as the effectiveness of antibiotics wanes. Biofabrication is a promising technique for producing innovative tissue structures that mimic the natural placement of biologically important substances, biocompatibility, mechanical properties, and repeatability. In this report, a novel method for generating three‐dimensional (3D) biomaterials for wound healing applications, utilizing an ulvan hydrogel is presented. Wet‐spun ulvan fibers and 3D printed hydrogel structure are fabricated through wet‐spinning and an additive manufacturing process. The results show that the viscosity of the ulvan solution is 110 Pa·s on day one and over 180 Pa·s on day four (gelation) which is critical for fiber spinning and additive manufacturing. The mechanical properties of ulvan fiber shows a 211% increase in elongation at break and a 350% increase in Young's modulus compared to wet‐spun alginate fibers. The biocompatibility evaluation demonstrates slightly superior cell viability levels for ulvan fibers when compared to commonly used biomaterials like alginate and chitosan for wound dressing applications. These results underscore the potential of ulvan fibers as a promising solution for wound healing, owing to their inherent biocompatibility.

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Development of rhamnose-rich hydrogels based on sulfated xylorhamno-uronic acid toward wound healing applications

Abstract: 3D printing of sulphated, rhamnose-rich hydrogels, using a seaweed extract from a controlled source of Australian ulvacean macroalgae, for wound healing.

Cited by 25 publications

References 38 publications

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

3D Bioprinting Constructs to Facilitate Skin Regeneration

Manufacturing Ulvan Biopolymer for Wound Dressings

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