Development of rGO encapsulated polymeric beads as drug delivery system for improved loading and controlled release of doxycycline drug

Singh, Geetanjali; Nenavathu, Bhavani Prasad

doi:10.1080/03639045.2020.1724137

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…rGO is widely used as a drug or gene delivery system and its use in breast cancer therapy has been suggested [20]. Several studies have shown cytotoxic effects of GO on normal, cancerous and bacterial cells, although toxicological information on rGO materials remains limited [11,13,21].…”

Section: Discussionmentioning

confidence: 99%

The Preliminary Study on the Proapoptotic Effect of Reduced Graphene Oxide in Breast Cancer Cell Lines

Krętowski

Jabłońska-Trypuć

Cechowska-Pasko

2021

IJMS

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Breast cancer is the most common cancer diagnosed in women, however traditional therapies have several side effects. This has led to an urgent need to explore novel drug approaches to treatment strategies such as graphene-based nanomaterials such as reduced graphene oxide (rGO). It was noticed as a potential drug due to its target selectivity, easy functionalisation, chemisensitisation, and high drug-loading capacity. rGO is widely used in many fields, including biological and biomedical, due to its unique physicochemical properties. However, the possible mechanisms of rGO toxicity remain unclear. In this paper, we present findings on the cytotoxic and antiproliferative effects of rGO and its ability to induce oxidative stress and apoptosis of breast cancer cell lines. We indicate that rGO induced time- and dose-dependent cytotoxicity in MDA-MB-231 and ZR-75-1 cell lines, but not in T-47D, MCF-7, Hs 578T cell lines. In rGO-treated MDA-MB-231 and ZR-75-1 cell lines, we noticed increased induction of apoptosis and necrosis. In addition, rGO has been found to cause oxidative stress, reduce proliferation, and induce structural changes in breast cancer cells. Taken together, these studies provide new insight into the mechanism of oxidative stress and apoptosis in breast cancer cells.

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Section: Discussionmentioning

confidence: 99%

The Preliminary Study on the Proapoptotic Effect of Reduced Graphene Oxide in Breast Cancer Cell Lines

Krętowski

Jabłońska-Trypuć

Cechowska-Pasko

2021

IJMS

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“…Delivery devices based on polymeric materials have been used for DOX release in several previous studies. In these studies, it was observed that most of DOX release from the polymer matrix occurred within the first 24–96 h (Anderson et al., 2015; Pal et al., 2019; Singh & Nenavathu, 2020; Tormos et al., 2015). For example, in one study an initial burst release of nearly 50% DOX from the drug‐loaded COL hydrogels was seen within the first 24 h (Pal et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial calcium phosphate cement with human periodontal ligament stem cell‐microbeads to enhance bone regeneration and combat infection

Qiu

Huang

Jin

et al. 2021

J Tissue Eng Regen Med

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Infectious bone defects remain a significant challenge in orthopedics and dentistry. Calcium phosphate cement (CPC) have attracted significant interest in use as local drug delivery system, which with great potential to control release of antibiotics for the treatment of infectious bone defects. Within the current study, a novel antibacterial scaffold of chitosan‐reinforced calcium phosphate cement delivering doxycycline hyclate (CPCC + DOX) was developed. Furthermore, the capacity of CPCC + DOX scaffolds for bone regeneration was enhanced by the human periodontal ligament stem cells (hPDLSCs) encapsulated in alginate beads. CPCC + DOX scaffolds were fabricated to contain different concentrations of DOX. Flexural strength of CPCC + DOX ranged from 5.56 ± 0.70 to 6.2 ± 0.72 MPa, which exceeded the reported strength of cancellous bone. Scaffolds exhibited continual DOX release, reaching 80% at 21 days. Scaffold with 5 mg/ml DOX (CPCC + DOX5mg) had a strong antibacterial effect, with a 4‐log colony forming unit reduction against S. aureus and P. gingivalis. The proliferation and osteogenic differentiation of hPDLSCs encapsulated in alginate hydrogel microbeads were investigated in culture with CPCC + DOX scaffolds. CPCC + DOX5mg had no negative effect on proliferation of hPDLSCs. Alkaline phosphatase activity, mineral synthesis, and osteogenic gene expressions for CPCC + DOX5mg group were much higher than control group. DOX did not compromise the osteogenic induction. In summary, the novel CPCC + DOX scaffold exhibited excellent mechanical properties and strong antibacterial activity, while supporting the proliferation and osteogenic differentiation of hPDLSCs. The CPCC + DOX + hPDLSCs construct is promising to enhance bone regeneration and combat bone infections in dental, craniofacial, and orthopedic applications.

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“…Research on rGO has demonstrated its unique role in DDS. 115–120 In one study, rGO was used as a carrier to load drugs through non-covalent bonding with folic acid and doxorubicin (DOX) to achieve targeting effects and inhibit cancer cell growth. 116 However, this DDS has limitations, including the system’s low targeting ability and inability to accurately detect DOX release.…”

Section: Biomedicine Application Of Gfnsmentioning

confidence: 99%

Potential Biomedical Limitations of Graphene Nanomaterials

Ban¹,

Hou²,

Shen³

et al. 2023

IJN

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Graphene-family nanomaterials (GFNs) possess mechanical stiffness, optical properties, and biocompatibility making them promising materials for biomedical applications. However, to realize the potential of graphene in biomedicine, it must overcome several challenges that arise when it enters the body’s circulatory system. Current research focuses on the development of tumor-targeting devices using graphene, but GFNs accumulated in different tissues and cells through different pathways, which can cause toxic reactions leading to cell apoptosis and body dysfunction when the accumulated amount exceeds a certain limit. In addition, as a foreign substance, graphene can induce complex inflammatory reactions with immune cells and inflammatory factors, potentially enhancing or impairing the body’s immune function. This review discusses the biomedical applications of graphene, the effects of graphene materials on human immune function, and the biotoxicity of graphene materials.

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Development of rGO encapsulated polymeric beads as drug delivery system for improved loading and controlled release of doxycycline drug

Cited by 10 publications

References 29 publications

The Preliminary Study on the Proapoptotic Effect of Reduced Graphene Oxide in Breast Cancer Cell Lines

The Preliminary Study on the Proapoptotic Effect of Reduced Graphene Oxide in Breast Cancer Cell Lines

Antibacterial calcium phosphate cement with human periodontal ligament stem cell‐microbeads to enhance bone regeneration and combat infection

Potential Biomedical Limitations of Graphene Nanomaterials

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