Development of retroviral vectors for insertional mutagenesis in medaka haploid cells

Lin, Fan; Liu, Qizhi; Yuan, Yusheng; Hong, Yunhan

doi:10.1016/j.gene.2015.07.059

Cited by 3 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, medaka has unique advantages over zebrafish: medaka can survive in a broad temperature range, and they have a well-known mechanism of sex differentiation (which should be considered in the study of adipogenesis) [ 48 ]. Finally, recent studies have reported that various techniques such as genetics, transgene, and genome editing by CRISPR/Cas system can be applied to medaka [ 49 – 52 ]. Therefore, further in vivo studies of adipogenesis in medaka by using these techniques will yield important insights into manipulating quality and quantity of adipose tissues in farmed fish and livestock animals.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a quantitative in vivo method for estimating adipose tissue volumes and the effects of dietary soy sauce oil on adipogenesis in medaka, Oryzias latipes

et al. 2018

View full text Add to dashboard Cite

Adipose tissue, which is conserved in higher eukaryotes, plays central roles in controlling the body’s energy balance, including excess energy storage and energy expenditure during starvation. In adipogenesis, intranuclear receptor, peroxisome proliferator–activated receptor gamma (PPARγ) is a key molecule, and PPARγ agonists can promote adipogenesis. Many studies on the in vitro screening of PPARγ agonists with compounds derived from various materials have been reported; however, in vivo assays for quick examination of these feeding effects have not been established. In this study, we developed a technique using a lipophilic fluorescent reagent, Nile red to quantitatively estimate the adipose tissue volumes by using Japanese rice fish, medaka (Oryzias latipes) and studied effects of dietary soy sauce oil (SSO), which is a discarded by-product from Japanese traditional food and is known to have PPARγ-agonistic activity, on adipogenesis. We found that SSO feeding increased the adipose tissue volumes, and the expression levels of adipogenesis-related genes increased in these medaka larvae. These results suggest that SSO feeding increases the adipose tissue volumes through adipogenesis promotion by PPARγ-agonistic activity in medaka, and medaka is a powerful model for studying adipogenesis. Furthermore, our study also demonstrates the availability of SSO as a dietary additive for farmed fish.

show abstract

Section: Discussionmentioning

confidence: 99%

Establishment of a quantitative in vivo method for estimating adipose tissue volumes and the effects of dietary soy sauce oil on adipogenesis in medaka, Oryzias latipes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Subsequently, the viral DNA was loaded for ddPCR analysis, and viral gene copy number was quantified by taking orf072 as the target gene. Meanwhile, the β-actin gene in host was quantified as a reference as previously described 37 . In brief, ddPCR assay mixtures (25 μl/reaction) containing 12.5 μl 2X EvaGreen Super Mix, 40–60 ng Hind III digested genomic DNA, and 100 nM primers ( Table S2 ) were loaded onto droplet generator (Bio-Rad) for droplets generation.…”

Section: Methodsmentioning

confidence: 99%

Singapore grouper iridovirus protein VP088 is essential for viral infectivity

Yuan

Yunzhi

Liu

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Viral infection is a great challenge in healthcare and agriculture. The Singapore grouper iridovirus (SGIV) is highly infectious to numerous marine fishes and increasingly threatens mariculture and wildlife conservation. SGIV intervention is not available because little is known about key players and their precise roles in SGVI infection. Here we report the precise role of VP088 as a key player in SGIV infection. VP088 was verified as an envelope protein encoded by late gene orf088. We show that SGIV could be neutralized with an antibody against VP088. Depletion or deletion of VP088 significantly suppresses SGIV infection without altering viral gene expression and host responses. By precisely quantifying the genome copy numbers of host cells and virions, we reveal that VP088 deletion dramatically reduces SGIV infectivity through inhibiting virus entry without altering viral pathogenicity, genome stability and replication and progeny virus release. These results pinpoint that VP088 is a key player in SGIV entry and represents an ideal target for SGIV intervention.

show abstract

Identification of Inhibitory Compounds Against Singapore Grouper Iridovirus Infection by Cell Viability-Based Screening Assay and Droplet Digital PCR

Yuan

Liu

et al. 2017

Mar Biotechnol

View full text Add to dashboard Cite

Singapore grouper iridovirus (SGIV) is one of the major causative agents of fish diseases and has caused significant economic losses in the aquaculture industry. There is currently no commercial vaccine or effective antiviral treatment against SGIV infection. Annually, an increasing number of small molecule compounds from various sources have been produced, and many are proved to be potential inhibitors against viruses. Here, a high-throughput in vitro cell viability-based screening assay was developed to identify antiviral compounds against SGIV using the luminescent-based CellTiter-Glo reagent in cultured grouper spleen cells by quantificational measurement of the cytopathic effects induced by SGIV infection. This assay was utilized to screen for potential SGIV inhibitors from five customized compounds which had been reported to be capable of inhibiting other viruses and 30 compounds isolated from various marine organisms, and three of them [ribavirin, harringtonine, and 2-hydroxytetradecanoic acid (2-HOM)] were identified to be effective on inhibiting SGIV infection, which was further confirmed with droplet digital PCR (ddPCR). In addition, the ddPCR results revealed that ribavirin and 2-HOM inhibited SGIV replication and entry in a dose-dependent manner, and harringtonine could reduce SGIV replication rather than entry at the working concentration without significant toxicity. These findings provided an easy and reliable cell viability-based screening assay to identify compounds with anti-SGIV effect and a way of studying the anti-SGIV mechanism of compounds.

show abstract

Development of retroviral vectors for insertional mutagenesis in medaka haploid cells

Cited by 3 publications

References 31 publications

Establishment of a quantitative in vivo method for estimating adipose tissue volumes and the effects of dietary soy sauce oil on adipogenesis in medaka, Oryzias latipes

Establishment of a quantitative in vivo method for estimating adipose tissue volumes and the effects of dietary soy sauce oil on adipogenesis in medaka, Oryzias latipes

Singapore grouper iridovirus protein VP088 is essential for viral infectivity

Identification of Inhibitory Compounds Against Singapore Grouper Iridovirus Infection by Cell Viability-Based Screening Assay and Droplet Digital PCR

Contact Info

Product

Resources

About