Development of Retinal Transplants

Sharma, Rajesh Kumar

doi:10.1034/j.1600-0420.1998.760229.x

Cited by 2 publications

(2 citation statements)

References 324 publications

(340 reference statements)

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“…Transplants survive at various transplantation sites, and they develop all the retinal layers and most of the cell types seen in the normal retina (Bergströ m 1984; Bergström et al 1994;Ehinger et al 1991Ehinger et al , 1992Seiler & Turner 1988). Many of the essential proteins and neurotransmitters have also been found in the transplants (previous reviews: del Cerro et al 1997;Ehinger et al 1996;Sharma et al 1995;Sharma 1998;, 1999.…”

Section: Transplantation Experimentsmentioning

confidence: 99%

Tapetoretinal degenerations: Experiences, experiments and expectations

Ehinger

2000

Acta Ophthalmologica Scandinavica

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ABSTRACT. Tapetoretinal degenerations are a common cause for vision problems, but have until recently not been amenable to rational treatment. With rapidly increasing insights into basic neurobiology and pathobiology this has now begun to change. From having been a relatively small group of largely unknown yet fairly prevalent disorders, they are rapidly forming a large set of well defined diseases, and it is easy to predict that our knowledge about them will continue to increase for many years to come. Vitamin A (15 000 IU daily) is currently the only rational treatment available. However, in experimental animals, therapy strategies are now actively being developed along several different lines. Apoptotic photoreceptor cell death can be delayed with different drugs, and at least one of them, diltiazem, is approved for human use in cardiovascular diseases. It remains to be seen if it has any clinically significant effect in human tapetoretinal degenerations. Other strategies aim at counteracting the production of harmful protein variants, acting either on DNA or mRNA levels. Transgenes can also be used to induce the production of important but missing metabolic components. Finally, cells or retina sheets can be transplanted, either to replace failing cells or as a source for missing trophic factors. Neither of these strategies has yet been transferred to humans, but trials are under way. With the high increase in the flow of new information on tapetoretinal disorders, much more precise diagnoses and much improved treatments are soon to be expected, augmenting considerably the possibilities for ophthalmologists to help patients with such diseases. It is not likely that there will be a single treatment for all the many varieties. Instead, we are most likely going to see pharmacological treatments for some of them, DNA transfers for some, and transplantations for others.

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Section: Transplantation Experimentsmentioning

confidence: 99%

Tapetoretinal degenerations: Experiences, experiments and expectations

Ehinger

2000

Acta Ophthalmologica Scandinavica

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“…The potential of stem cells to differentiate into a wide variety of neurons has raised the possibility that loss of other retinal neuron types, such as the RGCs, may also be amenable to similar therapies. Although the RGC cells do not survive embryonic retinal cell transplants [2][3][4], the use of embryonic stem cells [5] or embryonic RGCs [6] as donor tissue could possibly achieve this objective. Other possibilities include neural stem cells/progenitor cells that have been identified in different regions of the brain, including the retina [7].…”

Section: Introductionmentioning

confidence: 99%

CNS Targets Support and Sustain Differentiation of Cultured Neuronal and Retinal Progenitor Cells

2010

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Superior colliculus (SC) is the target of retinal neurons, allowing them to form connections. Cultured stem cells/progenitors can potentially be used as donor tissue to reconstruct degenerated retina including perhaps replacing lost ganglion cells in glaucoma. In which case, it will be essential for these cells to integrate with the central nervous system targets. Here, we have investigated if the mid-brain region containing superior colliculus (SC) provides a permissive environment for the survival and differentiation of neural progenitors, including retinal progenitor cells propagated in cultures. Neural (NPCs) and retinal progenitor cells (RPCs) from green fluorescent protein (GFP) transgenic mice were cultured. Passage two through four neural and retinal progenitor cells were subsequently cocultured with the SC organotypic slices and maintained in culture for 17 and eight days respectively. Differentiation of the neurons was studied by immunocytochemistry for retinotypic neuronal markers. Retinal progenitor cells cocultured with SC slices were able to differentiate into various neuronal morphologies. Some cocultured progenitor cells differentiated into neurons as suggested by class III β tubulin immunoreactivity. In addition, specific retinotypic neuronal differentiation of RPC was detected by immunoreactivity for calbindin and PKC. SC provides a permissive environment that supports survival and differentiation of the progenitor cells.

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Development of Retinal Transplants

Cited by 2 publications

References 324 publications

Tapetoretinal degenerations: Experiences, experiments and expectations

Tapetoretinal degenerations: Experiences, experiments and expectations

CNS Targets Support and Sustain Differentiation of Cultured Neuronal and Retinal Progenitor Cells

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