Development of respiration in yeast grown anaerobically on different carbon sources

Abstract

This paper describes the aerobic behaviour of yeast, previously grown anaerobically on a number of different carbon sources. The results show that yeast grown anaerobically on galactose retains its respiratory activity whereas the yeast grown similarly on glucose loses it. METHODS Maintenance and growth of yeast. Saccharomyces cere-Vi8siae strain no. 77 of the National Collection of Yeast Cultures (Brewing Industry Research Foundation, Nutfield, Surrey) was used in the present work. The organism was maintained aerobically on agar slopes containing inorganic salts, 2-25% (w/v) of Difco malt extract, 0.05% of Difco yeast extract and 0 5% of sucrose. The yeast was grown for 48 hr. at 300 and subcultured monthly. Growth of anaerobic cels8. The medium for the anaerobic bulk growth of the yeast contained (per 1.): glucose, 20 g.;

The influence of conditions of growth on the endogenous metabolism ofSaccharomyces cerevisiae: Effect on respiratory activity

“…Glucose in the growth medium at levels in excess of 0.05% (w/v) represses the synthesis of the majority of respiratory enzymes as well as those of the citric cycle so that it is only used as a biosynthetic pathway for the supply of essential intermediates (Polakis, Bartley and Meek, 1964). In addition, anaerobic growth on a glucose carbon source, results in the lack of an integrated respiratory chain (Criddle and Schatz, 1969), but Tustanoff and Bartley (1964a) have shown that yeast cells grown anaerobically on gal.actose in a medium supplemented with wheat-germ oil, Tween 80 and ergosterol, retain their ability to respire although the activity of cytochrome c oxidase in these cells was only one-third of that of aerobically grown cells. The repressive effect of glucose has been interpreted (Tustanoff and Bartley, 1964b) as a selective and direct effect on lipoprotein synthesis.…”

Microbial Water Relations. Effects of Solute Concentration on the Respiratory Activity of Sugar-tolerant and Non-tolerant Yeasts

“…There is nothing novel in the observation that growth in a relatively high glucose concentration represses respiratory activity and mitochondria1 formation in Saccharomyces cerevisiae (Slonimski, 1953 ;Ephrussi, Slonimski, Yotsuyanagi & Tavlitzki, 1956;Yotsuyanagi, 1962;Tustanoff & Bartley, 1964;Utter, Duel1 & Bernofsky, 1968). The comparison with the related species, S. rouxii, is of interest for several reasons, one being the common assumption that this type of catabolite repression is characteristic of the genus Saccharomyces.…”

DNA Content of Individual Mitochondrial Nucleoids Varies Depending on the Culture Conditions of the Yeast Saccharomyces cerevisiae

“…The anaerobic culture was performed as previously described (Shiiba et al 1997). The anaerobic (AN) medium used was basically the same medium as that used by Tustanoff and Bartley (1964), and consisted of 10.0 g yeast extract, 5.0 g casamino acids (Difco, vitamin-free), 9.0 g KH 2 PO 4 , 6.0 g (NH 4 ) 2 SO 4 , 0.5 g MgSO 4 7H 2 O, 0.3 g CaCl 2 , 5.83 g sodium lactate (60% w/w), 0.02 g ergosterol, 5.0 ml Tween 80, 0.6 mg wheat germ oil and 50 g glucose per liter (pH 5.3).…”