Development of resistance against blackleg disease in Brassica oleracea var. botrytis through in silico methods

Srivastava, Mugdha; Akhoon, Bashir Akhlaq; Gupta, Shishir; Gupta, Shailendra Kumar

doi:10.1016/j.fgb.2010.06.014

Cited by 10 publications

(5 citation statements)

References 54 publications

(43 reference statements)

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“…Simulations involving the search for conformations with the substrates on the surface of Pb ICL were limited to only a region surrounding the binding pocket of the protein, which was defined as involving the same residues of the template provided by [49] . Amino acids within the Pb ICL binding site (ASP24-GLN55 and ILE227-THR236) were 100% identical to the template An ICL.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Paracoccidioides lutzii Pb01 Isocitrate Lyase by the Natural Compound Argentilactone and Its Semi-Synthetic Derivatives

et al. 2014

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The dimorphic fungus Paracoccidioides spp. is responsible for paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America, causing serious public health problems. Adequate treatment of mycotic infections is difficult, since fungi are eukaryotic organisms with a structure and metabolism similar to those of eukaryotic hosts. In this way, specific fungus targets have become important to search of new antifungal compound. The role of the glyoxylate cycle and its enzymes in microbial virulence has been reported in many fungal pathogens, including Paracoccidioides spp. Here, we show the action of argentilactone and its semi-synthetic derivative reduced argentilactone on recombinant and native isocitrate lyase from Paracoccidioides lutzii Pb01 (PbICL) in the presence of different carbon sources, acetate and glucose. Additionally, argentilactone and its semi-synthetic derivative reduced argentilactone exhibited relevant inhibitory activity against P. lutzii Pb01 yeast cells and dose-dependently influenced the transition from the mycelium to yeast phase. The other oxygenated derivatives tested, epoxy argentilactone and diol argentilactone-, did not show inhibitory action on the fungus. The results were supported by in silico experiments.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Paracoccidioides lutzii Pb01 Isocitrate Lyase by the Natural Compound Argentilactone and Its Semi-Synthetic Derivatives

et al. 2014

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“…In Arabidopsis , approximately 10,000 orthologs have been detected in at least one reference species (i.e., in yeast, Drosophila , or humans) (Morsy et al 2008 ). Structure modeling and docking approaches have also been used to develop a peptide which can competitively inhibit the crucial glyoxylate pathway in fungi (Srivastava et al 2010 ).…”

Section: Different Plant-fungus Studiesmentioning

confidence: 99%

Application of Biotechnology and Bioinformatics Tools in Plant–Fungus Interactions

Srivastava¹,

Malviya

Dandekar³

2015

Plant Biology and Biotechnology

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Fungi have been recognized to be a major cause of disease in immunocompromised hosts: moreover, the loss of food and fodder crops through fungi has been unmatched since the last decade. Fungi colonize the plant cell and organs by modulating the host defense response. A number of different methods have been recently used to understand host-fungus interactions. With the advent of HiSeq approaches, more fungal genomes and transcriptomes are now sequenced, and their bioinformatics analyses have enriched and assisted our knowledge of the interplay between plant and fungi. The present chapter reviews the current biotechnological and bioinformatics approaches for the study of plant-fungus interactions.

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“…Protein-protein superposition, brings ligand into the target active site with an orientation similar to the one found in the original ligand-receptor crystal structure [39]. The adenine binding site of curcin was predicted by superposition of curcin with the ricin-adenine complex (PDB id: 2P8N).…”

Section: Active Site Predictionmentioning

confidence: 99%

Structure prediction and binding sites analysis of curcin protein of Jatropha curcas using computational approaches

et al. 2011

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Ribosome inactivating proteins (RIPs) are defense proteins in a number of higher-plant species that are directly targeted toward herbivores. Jatropha curcas is one of the biodiesel plants having RIPs. The Jatropha seed meal, after extraction of oil, is rich in curcin, a highly toxic RIP similar to ricin, which makes it unsuitable for animal feed. Although the toxicity of curcin is well documented in the literature, the detailed toxic properties and the 3D structure of curcin has not been determined by X-ray crystallography, NMR spectroscopy or any in silico techniques to date. In this pursuit, the structure of curcin was modeled by a composite approach of 3D structure prediction using threading and ab initio modeling. Assessment of model quality was assessed by methods which include Ramachandran plot analysis and Qmean score estimation. Further, we applied the protein-ligand docking approach to identify the r-RNA binding residue of curcin. The present work provides the first structural insight into the binding mode of r-RNA adenine to the curcin protein and forms the basis for designing future inhibitors of curcin. Cloning of a future peptide inhibitor within J. curcas can produce non-toxic varieties of J. curcas, which would make the seed-cake suitable as animal feed without curcin detoxification.

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Development of resistance against blackleg disease in Brassica oleracea var. botrytis through in silico methods

Cited by 10 publications

References 54 publications

Inhibition of Paracoccidioides lutzii Pb01 Isocitrate Lyase by the Natural Compound Argentilactone and Its Semi-Synthetic Derivatives

Inhibition of Paracoccidioides lutzii Pb01 Isocitrate Lyase by the Natural Compound Argentilactone and Its Semi-Synthetic Derivatives

Application of Biotechnology and Bioinformatics Tools in Plant–Fungus Interactions

Structure prediction and binding sites analysis of curcin protein of Jatropha curcas using computational approaches

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