Development of Recombinant Nucleocapsid Protein-based Enzyme-linked Immunosorbent Assay for Serological Detection of Winter Dysentery Disease

Singasa, Kanokwan; Songserm, Taweesak; Lertwatcharasarakul, Preeda; Jala, Siriluk; Phattanakunanan, Sakuna; Arunvipas, P.

doi:10.4172/2325-9590.1000249

Cited by 1 publication

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was lower than the herd seroprevalence found in the present study. The individual seroprevalence found in the present study was higher than in a previous study conducted in the western and central regions of Thailand that reported 88% (204/231) of cattle were positive to BCoV based on a commercial antibody ELISA test [2]. Because all herds were positive to BCoV antibodies, factors associated with the antibody detection at the herd level could not be determined.…”

Section: Discussioncontrasting

confidence: 92%

“…The sample size was calculated to determine the proportion of BCoV seropositive cattle, assuming an individual prevalence of 88%, based on a previous study in this area [2], with 95% confidence intervals, and 5% precision [15]. The sample size required was 163 heads.…”

Section: Sample Size Determinationmentioning

confidence: 99%

“…Bovine coronavirus (BCoV) belongs to the beta-coronavirus genus clade A that is closely related to a coronavirus known as bovine-like coronavirus (BCoV-like) originating from captive wild ruminants [1]. Outbreaks of BCoV are considered relatively contagious and have occurred around the world [2,3]. BCoV is transmitted by the fecal-oral route, aerosols, and respiratory droplets [4], and infects the respiratory and gastrointestinal tracts of cattle of all ages.…”

Section: Introductionmentioning

confidence: 99%

“…The diagnosis of BCoV can be conducted using viral culture, Available at www.veterinaryworld.org/Vol.14/August-2021/8.pdf antigen-captured enzyme-linked immunosorbent assay (ELISA), and hemagglutination assay [5]. According to reported sensitivity and specificity values, ELISA is the most suitable method to detect BCoV antibodies in cattle [2]. Important factors associated with the spread of BCoV within dairy herds are newly purchased animals and herd size.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the disease status should be determined. A study in Thailand, focusing on developing a recombinant nucleocapsid protein ELISA (rN protein) for the detection of BCoV antibodies in dairy cattle, reported that 88% of cattle in the western and central regions of Thailand were positive [2]. However, factors associated with the serological status were not identified.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Seroprevalence of bovine coronavirus and factors associated with the serological status in dairy cattle in the western region of Thailand

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background and Aim: Bovine coronavirus (BCoV) is a pathogen affecting the productivities of dairy cattle worldwide. The present study aimed to determine the seroprevalence and factors associated with BCoV serological status using a commercial indirect enzyme-linked immunosorbent assay (ELISA). Materials and Methods: A cross-sectional study was conducted in the western region of Thailand. Blood samples were collected from 30 dairy herds. In total, 617 blood serum samples were tested using a commercial indirect ELISA for BCoV-specific immunoglobulin G antibodies. A questionnaire was used to collect data on the factors which have been identified as risk factors for BCoV antibody detection. The age and history of diarrhea of each animal were recorded. Fisher's exact test was performed to univariately assess the association between BCoV serological status and possible risk factors. Variables with Fisher's exact test p<0.10 were then evaluated using multivariate logistic regression to identify factors associated with BCoV serological status. The Bonferroni adjustment was used for multiple comparisons of significant variables in the final multivariate logistic regression model. Results: No herd was free from antibodies to BCoV. The individual seroprevalence of BCoV was 97.89% (604/617). The prevalence within herds was in the range of 45.45-100%. Cattle >3 years of age were more likely to be seropositive to BCoV compared to cattle <1 year of age (p=0.003), with the odds ratio being 81.96. Disinfecting diarrhea stools were a protective factor for being BCoV seropositive, with odds ratios of 0.08 and 0.06 compared to doing nothing (p=0.008) and to clean with water (p=0.002), respectively. Conclusion: BCoV seropositive dairy cattle were distributed throughout the western region of Thailand. The probability of being seropositive for BCoV increased with increasing animal age. Cleaning the contaminated stool with appropriate disinfectants should be recommended to farmers to minimize the spread of the virus.

show abstract

Section: Discussioncontrasting

confidence: 92%

Section: Sample Size Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations