Development of recombinant matrix metalloproteinase-3 based sandwich ELISA for sero-diagnosis of canine mammary carcinomas

Pandey, Mamta; Kumar, B. V. Sunil; Singh, Sanjeev; Verma, Ramneek

doi:10.1080/15321819.2016.1211145

Cited by 8 publications

(6 citation statements)

References 18 publications

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“…To date, more than 2000 microsatellite markers have been reported, and many of them have been assigned to linkage groups and specific chromosomes [4]. Some studies have reported a few biomarkers for the diagnosis and prognosis of CMT [19,20]. However, there is a lack of rapid, sensitive and specific biomarkers, which are required for the diagnosis of carcinogenesis in earlier stages.…”

Section: Discussionmentioning

confidence: 99%

Microsatellite Instability and MMR Genes Abnormalities in Canine Mammary Gland Tumors

et al. 2020

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Early diagnosis of mammary gland tumors is a challenging task in animals, especially in unspayed dogs. Hence, this study investigated the role of microsatellite instability (MSI), MMR gene mRNA transcript levels and SNPs of MMR genes in canine mammary gland tumors (CMT). A total of 77 microsatellite (MS) markers in 23 primary CMT were selected from four breeds of dogs. The results revealed that 11 out of 77 MS markers were unstable and showed MSI in all the tumors (at least at one locus), while the other markers were stable. Compared to the other markers, the ABC9TETRA, MEPIA, 9A5, SCNA11 and FJL25 markers showed higher frequencies of instability. All CMT demonstrated MSI, with eight tumors presenting MSI-H. The RT-qPCR results revealed significant upregulation of the mRNA levels of cMSH3, cMLH1, and cPMSI, but downregulation of cMSH2 compared to the levels in the control group. Moreover, single nucleotide polymorphisms (SNPs) were observed in the cMSH2 gene in four exons, i.e., 2, 6, 15, and 16. In conclusion, MSI, overexpression of MMR genes and SNPs in the MMR gene are associated with CMT and could be served as diagnostic biomarkers for CMT in the future.

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Section: Discussionmentioning

confidence: 99%

Microsatellite Instability and MMR Genes Abnormalities in Canine Mammary Gland Tumors

et al. 2020

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“…Hence, need of the hour is to add a few more specific CMT biomarkers to the existing panel for accurate diagnosis and prognosis of the disease. In the last few years, our laboratory has put considerable efforts in search of markers for non-invasive diagnosis and prognosis of CMT (Sunil Kumar et al 2013Pandey et al 2015Pandey et al , 2017Pandey et al , 2018Kaur et al 2016). Advanced research in cancer biology has shown the importance of Hsps as biomarkers of carcinogenesis and their potential in prognosis of some human cancer types (Salda and Romanucci 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, booster doses were given on 7th, 14th, 21st, and 28th days after priming with recombinant proteins mixed with Freund's incomplete adjuvant (Santacruz, USA). Test bleeding was done from tail veins of mice on 29th day to check the titre of the antibodies (Pandey et al 2017). Final bleeding was done by cardiac puncture on 30th day, and sera were harvested and stored at − 20 °C until further use.…”

Section: Production Of Hyperimmune Sera Against Canine Rhsp27 In Micementioning

confidence: 99%

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

et al. 2019

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In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimmune serum against the protein in mice. Using the hyperimmune serum, an indirect ELISA was developed to estimate circulating levels of Hsp27 in dogs with various types of mammary neoplasia and to compare their levels with those of tumor-free apparently healthy dogs. The developed assay had a high specificity (83.3%) and sensitivity (96.6%) for a cut-off value of 0.45 with respect to histopathological examination in discriminating healthy dogs from those bearing mammary tumors. Serum Hsp27 levels were found to be significantly elevated in tumor subjects (20.33 ± 1.23 ng/ml) as compared to healthy controls (6.56 ± 0.51 ng/ml) and the increase in the Hsp27 levels was irrespective of mammary tumor histotypes. However, dogs with grade-II tumors had higher Hsp27 levels as compared to grade-I types. Therefore, Hsp27 can be exploited as one of the 'neoplastic signatures' of canine mammary tumors.

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“…Focusing on CMN, according to the consulted literature, over-expression of MMP3 has been described in serum [80] and tissue [69] samples from bitches with mammary carcinoma. The present results confirm that MMP3 plays a role in CMN development since it is over-expressed in both benign and malignant mammary tumours.…”

Section: Discussionmentioning

confidence: 99%

Programmed Death-Ligand (PD-L1), Epidermal Growth Factor (EGF), Relaxin, and Matrix Metalloproteinase-3 (MMP3): Potential Biomarkers of Malignancy in Canine Mammary Neoplasia

Galadima,

Teles,

Pastor

et al. 2024

IJMS

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Gene expression has been suggested as a putative tool for prognosis and diagnosis in canine mammary neoplasia (CMNs). In the present study, 58 formalin-fixed paraffin-embedded (FFPE) paraffined canine mammary neoplasias from 27 different bitches were included. Thirty-seven tumours were classified as benign, whereas thirty-one were classified as different types of canine carcinoma. In addition, mammary samples from three healthy bitches were also included. The gene expression for vascular endothelial growth factor-α (VEGFα), CD20, progesterone receptor (PGR), hyaluronidase-1 (HYAL-1), programmed death-ligand 1 (PD-L1), epidermal growth factor (EGF), relaxin (RLN2), and matrix metalloproteinase-3 (MMP3) was assessed through RT-qPCR. All the assessed genes yielded a higher expression in neoplastic mammary tissue than in healthy tissue. All the evaluated genes were overexpressed in neoplastic mammary tissue, suggesting a role in the process of tumorigenesis. Moreover, PD-L1, EGF, relaxin, and MMP3 were significantly overexpressed in malignant CMNs compared to benign CMNs, suggesting they may be useful as malignancy biomarkers.

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Development of recombinant matrix metalloproteinase-3 based sandwich ELISA for sero-diagnosis of canine mammary carcinomas

Cited by 8 publications

References 18 publications

Microsatellite Instability and MMR Genes Abnormalities in Canine Mammary Gland Tumors

Microsatellite Instability and MMR Genes Abnormalities in Canine Mammary Gland Tumors

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

Programmed Death-Ligand (PD-L1), Epidermal Growth Factor (EGF), Relaxin, and Matrix Metalloproteinase-3 (MMP3): Potential Biomarkers of Malignancy in Canine Mammary Neoplasia

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