Development of recombinant high yielding lines with improved protein content in rice (Oryza sativaL.)

Abstract

Rice has the lowest grain protein content (GPC) among cereals. Efforts have been made to improve GPC through the modified bulk-pedigree method of selection. A total of 1780 F8 recombinant lines were derived in the year 2013 from five different cross combinations involving two high-GPC landraces, namely ARC10075 and ARC10063, three high-yielding parents, namely Swarna, Naveen and IR64, and one parent, namely Sharbati, known for superior grain quality with high micronutrient content. Near-infrared spectroscopy was used to facilitate high-throughput selection for GPC. Significant selection differential, response to selection and non-significant differences between the predicted and observed response to selection for GPC and protein yield indicated the effectiveness of this selection process. This resulted in lines with high GPC, protein yield and desirable levels of amylose content. Further, based on high mean and stability for GPC and protein yield over the environments in the wet seasons of 2013, 2014 and the dry season of 2014, 12 elite lines were identified. Higher accumulation of glutelin fraction and non-significant change in prolamin/glutelin ratio in the grain suggested safe guarding of the nutritional value of rice grain protein of most of these identified lines. Since rice is the staple food of millions, the output of breeding for high GPC could have a significant role in alleviating protein malnutrition, especially in the developing world.

Mapping genetic determinants for grain physicochemical and nutritional traits in brown and pigmented rice using genome-wide association analysis

“…Similar kind of reports for AC, GT and GC were available in public domain(Ponce et al 2018;Yang et al 2018).In general, the anti-waxy gene increases protein content and decreases the amylose content of rice grains(Li et al 2009). A negative association of grain protein and amylose content has been observed in previous studies(Yang et al 2004;Chattopadhyay et al 2018). In the present association analysis, we could not detect markers in association with Wx locus.…”

Mapping genetic determinants for grain physicochemical and nutritional traits in unpolished rice using genome-wide association analysis

“…Zhang et al (2020) detected a major QTL over environments for GC (qGC10.1) using a RIL population derived from a cross between PA64s and 93-11suitable for ne mapping further.Associated loci for GPC and grain qualityIn general, the anti-waxy gene increases protein content and decreases the amylose content of rice grains(Li et al 2009). A negative association of grain protein and amylose content has been observed in previous studies(Yang et al 2004;Chattopadhyay et al 2018). In the present association analysis, we could not detect markers in association with Wx locus.…”

OsASN1 Overexpression in Rice Increases Grain Protein Content and Yield under Nitrogen-Limiting Conditions

“…In addition, although the protein content of rice grains is relatively low compared with other cereal crops ( Mahender et al. 2016) , the protein quality of rice grains is superior due to a better balanced amino acid profile and the presence in high amounts of highly nutritive and digestible glutelins ( Chattopadhyay et al. 2018) .…”