Development of real-time RT–PCR for evaluation of JEV clearance during purification of HPV type 16 L1 virus-like particles

Functional Modulation of Dendritic Cells and Macrophages by Japanese Encephalitis Virus through MyD88 Adaptor Molecule-Dependent and -Independent Pathways

“…The defined peptides of chicken OVA, OVA 257-264 (SIINFEKL) and OVA 323-339 (ISQAVHAAHAEINEAGR), and immunodominant peptide (glycoprotein B (gB) 498 -505 , SSIEFARL) of HSV-1 gB were chemically synthesized at Peptron. JEV-specific primers for the detection of viral RNA (JEV 10,564 -10,583 forward, 5Ј-CCC TCA GAA CCG TCT CGG AA-3Ј and JEV 10,886 reverse, 5Ј-CTA TTC CCA GGT GTC AAT ATG CTG T-3Ј) (35) were synthesized at Bioneer.…”

Construction of an infectious molecular clone of Japanese encephalitis virus genotype V and its derivative subgenomic replicon capable of expressing a foreign gene

“…Then, 2 l of the RNA solution was subjected to reverse transcription in 10 l volume with the Prime Script TM RT reagent Kit (TaKaRa Bio Inc., Shiga, Japan) according to the manufacturer's instruction. The 2 l of the obtained cDNA was amplified by PCR with primers #13 and #14 (Table 1) by using SYBR ® Premix Ex Taq TM (TaKaRa Bio Inc.) according to the manufacturer's instruction (Jeong et al, 2003). PCR amplification was measured in real time on a Thermal Cycler Dice TM Real time system (TaKaRa Bio Inc.), and the crossing point (CP) values were determined.…”

Multifront Assault on Antigen Presentation by Japanese Encephalitis Virus Subverts CD8+ T Cell Responses

“…cDNA prepared from 2 mg RNA was used in a reaction with specific primers (JEV-F 10564-10583 : 59-CCC TCA GAA CCG TCT CGG AA-39 and JEV-R 10862-10886 : 59-CTA TTC CCA GGT GTC AAT ATG CTG T-39) (30). The reactions were denatured at 95˚C for 10 min and then subjected to 50 cycles of 95˚C for 30 s, 58˚C for 30 s, and 72˚C for 30 s. After the reaction cycle was completed, the temperature was increased from 65˚C to 95˚C at a rate of 1˚C/min, and the fluorescence was measured every 15 s to construct a melting curve.…”