Development of Real-Time PCR Assays for Detection and Quantification ofBacillus cereusGroup Species: Differentiation ofB. weihenstephanensisand RhizoidB. pseudomycoidesIsolates from Milk

Oliwa-Stasiak, K.; Kolaj-Robin, Olga; Adley, Catherine C.

doi:10.1128/aem.01581-10

Cited by 21 publications

(10 citation statements)

References 28 publications

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“…MAA is the only acute phase protein produced in response to bacterial infection in the udder and is therefore an immediate and direct indicates of infection [93]. PCR (polymerase chain reaction) is used for bacteria identification and differentiation [94,95]. Gas chromatography/mass spectrometry can be used for analysis for metabolomics characterization of milk [96,97].…”

Section: Industry 40 In the Dairy Industrymentioning

confidence: 99%

The Dairy Industry: Process, Monitoring, Standards, and Quality

Burke¹,

Zacharski²,

Southern³

et al. 2018

Descriptive Food Science

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Sampling and analysis occur along the milk processing train: from collection at farm level, to intake at the diary plant, the processing steps, and the end products. Milk has a short shelf life; however, products such as milk powders have allowed a global industry to be developed. Quality control tests are vital to support activities for hygiene and food standards to meet regulatory and customer demands. Multiples of chemical and microbiological contamination tests are undertaken. Hazard analysis testing strategies are necessary, but some tests may be redundant; it is therefore vital to identify product optimization quality control strategies. The time taken to undergo testing and turnaround time are rarely measured. The dairy industry is a traditional industry with a low margin commodity. Industry 4.0 vision for dairy manufacturing is to introduce the aspects of operational excellence and implementation of information and communications technologies. The dairy industries' reply to Industry 4.0 is represented predominantly by proactive maintenance and optimization of production and logistical chains, such as robotic milking machines and processing and packaging line automation reinforced by sensors for rapid chemical and microbial analysis with improved and real-time data management. This chapter reviews the processing trains with suggestions for improved optimization.

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Section: Industry 40 In the Dairy Industrymentioning

confidence: 99%

The Dairy Industry: Process, Monitoring, Standards, and Quality

Burke¹,

Zacharski²,

Southern³

et al. 2018

Descriptive Food Science

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“…Other primers and probes targeting the chromosome have been designed for specific detection and quantification of the Bcg as well as differentiation among Bcg species (43,44). However, our BLAST similarity searches on WGS databases in NCBI revealed that these Bcg primers missed a certain proportion of Bcg strains whose genome sequences have been published recently (22 April 2015) (data not shown).…”

Section: Discussionmentioning

confidence: 90%

Chromosome-Directed PCR-Based Detection and Quantification of Bacillus cereus Group Members with Focus on B. thuringiensis Serovar israelensis Active against Nematoceran Larvae

Schneider

Hendriksen

Melin

et al. 2015

Appl Environ Microbiol

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e Bacillus thuringiensis serovar israelensis is a wide-spread soil bacterium affiliated with the B. cereus group (Bcg) and is widely used in biocontrol products applied against mosquito and black fly larvae. For monitoring and quantification of applied B. thuringiensis serovar israelensis and its effect on indigenous B. thuringiensis serovar israelensis and Bcg assemblages, efficient and reliable tools are essential. The abundance and properties of B. thuringiensis serovar israelensis strains in the environment traditionally have been investigated with cultivation-dependent techniques, which are hampered by low sensitivity and the morphological similarity between B. cereus and B. thuringiensis. Currently available PCR-based detection and quantification tools target markers located on plasmids. In this study, a new cultivation-independent PCR-based method for efficient and specific quantification of B. thuringiensis serovar israelensis and Bcg is presented, utilizing two sets of PCR primers targeting the bacterial chromosome. Sequence database searches and empirical tests performed on target and nontarget species, as well as on bulk soil DNA samples, demonstrated that this diagnostic tool is specific for B. thuringiensis serovar israelensis and Bcg. The method will be useful for comparisons of Bcg and B. thuringiensis serovar israelensis abundances in the same samples. Moreover, the effect of B. thuringiensis serovar israelensis-based insecticide application on the total Bcg assemblages, including indigenous populations, can be investigated. This type of information is valuable in risk assessment and policy making for use of B. thuringiensis serovar israelensis in the environment. The bacterium Bacillus thuringiensis (Bt) is one of the grand successes in microbial pest control (1). After its first discovery in 1901 and description by Berliner in 1911, the first biocontrol product based on Bt, Sporein, for control of the European corn borer (Ostrinia nubilalis), was commercially available already in 1938 (1). The crystalline, proteinaceous ␦-endotoxins of Bt are formed during sporulation, and their lethal effect is manifested only after larval ingestion of spores or free crystals (2, 3). Bt is a member of the Bacillus cereus group (Bcg), which consists of the Gram-positive rod-shaped spore-forming bacterial species B. cereus, Bt, B. anthracis, B. mycoides, B. pseudomycoides, and B. weihenstephanensis (4,5). However, a multidatatype phylogenetic analysis of Bcg strains isolated from various sample types, including environmental, clinical, food, and diary sources, did not reflect current Bcg taxonomy well. This finding indicated that the phylogeny of this bacterial group is more complicated than reflected by the division into the current six species (6).The bacterium B. thuringiensis serovar israelensis produces toxins active against nematoceran larvae and has been widely used for biocontrol of mosquitoes and black flies (3). B. thuringiensis serovar israelensis was described in 1976 (7) and has been shown to have ...

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“…d) were 0.9985 and 0.9992, respectively. These linear relationships indicate that the Ct values are a reliable basis for estimating the gene concentrations (Oliwa‐Stasiak et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Monitoring of the bacterial communities of bamboo shoots (Dendrocalamus latiflorus) during pickling process

Xia

Chunxia

et al. 2017

Int J of Food Sci Tech

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Summary The diversity and dynamics of the dominant bacterial communities arising during the pickling process of bamboo shoots (Dendrocalamus latiflorus) were investigated by nested polymerase chain reaction denaturing gradient gel electrophoresis combined with quantitative real‐time polymerase chain reaction. Results showed only several kinds of halophilic bacteria during early sampling time (0−3 days). After pickling for 7 days, Lactococcus lactis significantly increased (P < 0.05, quantities were 6.39 × 105 copies μL−1) and became the first dominant bacterium. After pickling for 14 days, Weissella sp. bands appeared and quickly became dominant on the 21st day (4.07 × 106 copies μL−1). As maturation progressed, Lc. lactis decreased in intensity whereas Weissella sp. increased in intensity. Finally, the quantities of Weissella sp. (2.50 × 107 copies μL−1) became higher than those of Lc. lactis (1.43 × 107 copies μL−1) after pickling for 56 days. The pickling process was initiated by Lc. lactis, followed by Lc. lactis and Weissella sp., and was finally succeeded by Weissella sp.

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Development of Real-Time PCR Assays for Detection and Quantification ofBacillus cereusGroup Species: Differentiation ofB. weihenstephanensisand RhizoidB. pseudomycoidesIsolates from Milk

Cited by 21 publications

References 28 publications

The Dairy Industry: Process, Monitoring, Standards, and Quality

The Dairy Industry: Process, Monitoring, Standards, and Quality

Chromosome-Directed PCR-Based Detection and Quantification of Bacillus cereus Group Members with Focus on B. thuringiensis Serovar israelensis Active against Nematoceran Larvae

Monitoring of the bacterial communities of bamboo shoots (Dendrocalamus latiflorus) during pickling process

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