Development of RapidHIT® ID using NGMSElect™ Express chemistry for the processing of reference samples within the UK criminal justice system

Validation of the Applied Biosystems RapidHIT ID instrument and ACE GlobalFiler Express sample cartridge

“…Similar results (i.e., detection of primer flare peaks, dye blobs, artifacts, “anomaly peaks”, etc.) have been documented in previous studies [ 14 , 22 , 42 ]. For this study, these artifacts did not affect the ability to accurately genotype the DNA positive samples but were more readily visible in the negative control samples.…”

“…The other is the Applied Biosystems RapidHIT ID System (Thermo Fisher Scientific, Waltham, MA, USA), which was formerly a product of IntegenX (Pleasanton, CA, USA) [ 42 – 46 ]. Previous changes/updates to the RapidHIT ID System instrumentation are the use of different PCR STR typing kits [ 22 – 24 ] and a single sample analysis design [ 14 , 22 ]. In the past few years, the RapidHIT ID instrument has been modified again compared with the previous versions.…”

Recent trends and developments in forensic DNA extraction

“…To cater to low throughput needs in a decentralized environments such as police stations or border crossing stations (Salceda et al, 2017), Thermo Fisher also launched the Applied Biosystems RapidHIT ID System that generates STR profiles from one reference sample at a time within 90 min, with either the ACE or EXT microfluidic cartridge, both of which are single‐use (Buscaino et al, 2018; Salceda et al, 2017). The RapidHIT ID with the ACE cartridge has been validated to process reference buccal samples (Amick & Swiger, 2019; Salceda et al, 2017; Shackleton, Gray, Ives, Malsom, & Vanhinsbergh, 2019; Wiley et al, 2017). DNA extraction is performed in the swab chamber of the cartridge (Buscaino et al, 2018; Salceda et al, 2017; Wiley et al, 2017).…”

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

“…ACE and RapidINTEL utilize the GlobalFiler set of 21 autosomal STRs, one Y-STR, one Y-chromosome InDel, and amelogenin. Publication Instrument Cartridge/Kit Test Performed and Success Rates Reported Amick & Swiger 2019 [ 56 ] RapidHIT ID ACE and EXT Performed SWGDAM internal validation studies including known and database-type samples, reproducibility, precision, sensitivity, stochastic effects, mixtures, contamination assessment, and concordance studies Carney et al, 2019 [ 57 ] ANDE 6C A-Chip Conducted SWGDAM developmental validation (across 6 labs, 2045 swabs, 13 instruments): species specificity, limit of detection, stability, inhibitors, reproducibility, reference material, mixtures, precision, concordance, signal strength, peak height ratio, stutter, non-template addition, resolution, and contamination assessment; first-pass success rate (1338 samples with 20 CODIS core loci) = 92% ; successfully interpreted >2000 samples with over 99.99% concordant alleles; data package led to receiving NDIS approval in June 2018 Shackleton et al, 2019 [ 58 ] RapidHIT ID NGM SElect Express Described development studies that included process optimization, sensitivity, repeatability, contamination checks, inhibition, swab age, concordance, and overall performance; success rate (124 samples) = 84.5% gave a full profile Shackleton et al, 2019 [ 59 ] RapidHIT 200 NGM SElect Express Performed some protocol adjustments that enhanced slightly the sensitivity with mock crime scene samples (dilutions of blood and cell line DNA) Yang et al, 2019 [ 53 ] MiDAS PowerPlex ESI 16 Plus Described protocols for analysis of reference samples with a fully automated integrated microfluidic system (MiDAS), which is not commercially available Romsos et al, 2020 [ 48 ] ANDE 6C, RapidHIT ID, RapidHIT 200 A-Chip, ACE Reported results from the July 2018 rapid DNA maturity assessment with multiple instruments organized by NIST; the average success rate for obtaining the 20 CODIS core loci was 85% (n=240) Manzella & Moreno 2020 [ …”